Abstract
The question of which dendritic cells (DCs) respond to pulmonary antigens and cross-prime CD8+ T cells remains controversial. We show here that influenza-specific CD8+ T cell priming was controlled by different DCs at different times after infection. Whereas early priming was controlled by both CD103+CD11blo and CD103−CD11bhi DCs, CD103−CD11bhi DCs dominated antigen presentation at the peak of infection. Moreover, CD103−CD11bhi DCs captured exogenous antigens in the lungs and directly cross-primed CD8+ T cells in the draining lymph nodes without transferring antigen to CD8α+ DCs. Finally, we show that CD103−CD11bhi DCs were the only DCs to express CD70 after influenza infection and that CD70 expression on CD103−CD11bhi DCs licensed them to expand CD8+ T cell populations responding to both influenza and exogenous ovalbumin.
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Change history
07 May 2010
In the version of this article initially published, the label along the vertical axis of Figure 6h was incorrect. The correct label is “OT-II proliferating cells (×102).” The error has been corrected in the HTML and PDF versions of the article.
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Acknowledgements
We thank L. LaMere and K. Martin for animal husbandry, and the members of the University of Rochester Medical Center Flow Cytometry Core Facility for cell sorting. Supported by the University of Rochester and the National Institutes of Health (AI61511 and HL69409 to T.D.R., and AI06856 to F.E.L.).
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A.B.-T. designed and did the experiments, analyzed the data and wrote the paper; B.L. optimized the DC-subsetting protocol and helped interpret the data; F.E.L. helped supervise the project and edit the paper and T.D.R. supervised the project, helped design the experiments and edited the paper.
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Ballesteros-Tato, A., León, B., Lund, F. et al. Temporal changes in dendritic cell subsets, cross-priming and costimulation via CD70 control CD8+ T cell responses to influenza. Nat Immunol 11, 216–224 (2010). https://doi.org/10.1038/ni.1838
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DOI: https://doi.org/10.1038/ni.1838
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