Abstract
Web spiders assemble spidroin monomers into silk fibres of unrivalled tensile strength at remarkably high spinning speeds of up to 1 m s−1. The spidroin N-terminal domain contains a charge-driven, pH-sensitive relay that controls self-association by an elusive mechanism. The underlying kinetics have not yet been reported. Here we engineer a fluorescence switch into the isolated N-terminal domain from spidroin 1 of the major ampullate gland of the nursery web spider E. australis that monitors dimerization. We observe ultrafast association that is surprisingly insensitive to salt, contrasting the classical screening effects in accelerated, charged protein interfaces. To gain deeper mechanistic insight, we mutate each of the protonatable residue side chains and probe their contributions. Two vicinal aspartic acids are critically involved in an unusual process of accelerated protein association that is protected from screening by electrolytes, potentially facilitating the rapid synthesis of silk fibres by web spiders.
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Introduction
Orb-web-weaving spiders use seven specialized glands to spin silk threads tailored for various tasks including prey capture, reproduction and shelter1. Dragline silk from the major ampullate gland forms the main structural web fibre and spider’s lifeline. This thread is exceptionally tough and supersedes mechanical properties of man-made material2,3,4. As such, dragline silk is a focus of biotechnology and biomimetic material sciences. To mimic or reproduce spider silk, it is crucial to understand the structure–function relationship of the constituent proteins, the way they are processed in the spider’s spinneret and the interactions they form in fibres. Spider silk fibres are polymers made of protein monomers, so-called spidroins. The bulk of a spidroin sequence consists of repetitive peptide motifs of simple amino-acid composition. This central segment is flanked by non-repetitive, globular carboxy- and amino-terminal domains3,5. Spidroins have a molecular weight of about 300 kDa1 and the non-repetitive terminal domains cover only ~10% of the sequence area but have critical roles during silk synthesis5. At the beginning of the spinning process, a highly concentrated solution of spidroins is stored in the ampulla of the spinning gland. During silk extrusion, spidroins pass through a tapering duct where they undergo controlled phase and structural transitions before they leave the exit spigot as solid fibre1. On their journey through the duct, proteins experience a series of mechanical and chemical stimuli. Shear forces align spidroins mechanically and changes of solution composition induce structural transitions1,4. The molecular basis of these transitions is only partly understood. The central sequence segment is largely disordered in the gland but forms regular and non-regular secondary structure in solid silk fibres1,3. NMR structural studies show that the carboxy-terminal domain forms a covalent, parallel homodimer that is stabilized by cross-domain disulphides, thus connecting two spidroins covalently6. The covalent linkage leads to dimeric strings of spidroins in solution that are terminated by the amino-terminal domains (NTDs) on opposite ends. The NTDs contain a pH-sensitive relay7 that triggers anti-parallel homodimerization on solution acidification at physiological pH. A change of pH from 7 to 6 is induced by proton pumps located at the epithelial cells in the distal part of the spinning duct, just before spidroins leave the exit spigot as silk fibre1,8. pH-triggered NTD dimerization leads to polymerization of spidroins during their passage through the acidified assembly zone, forming supermolecules in solid silk threads7,9,10 (Fig. 1). The molecular details of this process are not yet established.
Here we study the kinetics of association of the isolated NTD from spidroin 1 of the major ampullate gland of the nursery web spider Euprosthenops australis by means of fluorescence experiments. We engineer an extrinsic fluorescence switch that signals assembly into the NTD and emulate a change of solution pH, similar to as it happens in the spinning duct, through rapid-mixing experiments. We measure association and dissociation rate constants and their dependence on solution ionic strength (IS). We further mutate all protonatable residue side chains of the NTD to probe their contributions to assembly. Two vicinal aspartic acids emerge as critical in a pH-triggered process of accelerated protein association that is surprisingly insensitive to IS. We discuss potential implications of our findings in the rapid synthesis of silk fibres by web spiders.
Results
Design of a fluorescence reporter for NTD assembly
The NTD of spidroin 1 from the major ampullate gland of the nursery web spider E. australis is a 14-kDa five-helix bundle that forms a homodimer7. The anti-parallel symmetry of the association interface brings the side chain of glutamine at position 50 (Q50), which flanks the interface, within 1-nm distance to itself on the opposing subunit (Fig. 2a). Organic fluorophores at such short distance form non-covalent dimers with substantially altered absorption and fluorescence emission characteristics11. We mutated Q50 to cysteine (Q50C) and chemically modified the mutant at the cysteine side chain with the thiol-reactive, red-absorbing oxazine fluorophore AttoOxa11 (F). Figure 2b shows absorption and fluorescence emission spectra of AttoOxa11-modified NTD (NTDF) recorded in buffered solutions where the NTD is monomeric and dimeric, that is, in 50 mM phosphate pH 7.0 and in 20 mM 2-(N-morpholino)ethanesulphonic acid (MES) pH 6.0, respectively. At pH 7.0, NTDF shows the common absorption/emission characteristics of AttoOxa11. At pH 6.0, however, the absorptivity at the maximum wavelength of ~660 nm is substantially reduced with respect to the lower-wavelength absorption band at ~610 nm. These spectral changes are accompanied by a dramatic loss of fluorescence emission (Fig. 2b) characteristic of dimeric fluorophores in π-stacking H-type interaction geometry11,12. The dramatically changed spectral properties of H-type fluorophore dimers is explained by a delocalized electronic excitation, that is, a molecular exciton11. We conclude that AttoOxa11 at position Q50C yields a sensitive and specific probe for NTD dimerization.
Kinetics of NTD association and dissociation
Dimerization of wild-type NTD can be followed by intrinsic fluorescence. A single tryptophan (Trp) side chain at the N terminus of the NTD is buried in the monomer but changes conformation to take a more solvent-exposed position in the dimer13. As a consequence, intrinsic Trp fluorescence emission is quenched on NTD dimerization7,10,13. We applied stopped-flow fluorescence spectroscopy to measure kinetics of NTD assembly using both intrinsic and extrinsic probes. We measured association kinetics of wild-type NTD by monitoring Trp and of NTDF by monitoring AttoOxa11 fluorescence. Samples were prepared in 10 mM MES pH 7.0 with the IS adjusted to 150 mM and rapidly mixed into 50 mM MES pH 6.0 passing through an optical stopped-flow cell. The experiment emulated conditions in the spinneret of web spiders where proton pumps in the distal part of the spinning duct initiate a change of solution pH from 7 to 6 (refs 1, 8) (Fig. 1). We observe kinetic transients of fluorescence quenching resulting from NTD association, which fitted well to exponential decay functions (Fig. 3a). Figure 3b,c shows the observed rate constants plotted versus sample concentration. All kinetic transients of association of NTD and NTDF together with data fits are provided as Supplementary Figs S1 and S2. Observed rate constants increased linearly with sample concentration, as expected for a bimolecular event (Fig. 3b,c). The slopes of linear fits to the data yield association rate constants, ka, of 2.6±0.5 × 108 M−1 s−1 for NTD and of 3.0±0.2 × 108 M−1 s−1 for NTDF. The good agreement of rate constants measured from intrinsic Trp and extrinsic AttoOxa11 fluorescence shows that extrinsic modification does not perturb NTD association.
The NTD dissociation rate constant, kd, cannot be obtained under the physiologically relevant conditions at pH 6 from intrinsic Trp fluorescence. However, the engineered extrinsic probe provides a unique tool to measure kd at pH 6 using chasing experiments. We prepared dimeric NTDF in pH 6.0-buffered solution and chased one subunit off the dimer by rapidly mixing-in excess of non-modified NTD using the stopped-flow technique. Since the monomer/dimer equilibrium is dynamic and since under these conditions ka is much larger than kd, every dissociation event of dimeric NTDF results in rapid reassociation with non-modified NTD, present at excess concentration, until the equilibrium is reached and all fluorescence-quenched NTDF/NTDF dimers exchanged to fluorescent NTDF/NTD assemblies. The resulting fluorescence increase signals NTDF dissociation. Measured kinetic transients of dissociation fit well to exponential rise functions (Fig. 3d). To further test whether the observed kinetics truly reflected dissociation, we measured transients at various sample concentrations. The kd determined for 25, 50 and 100 nM NTDF in 50 mM MES pH 6.0 was 0.3±0.1, 0.33±0.05 and 0.26±0.05 s−1, respectively, values which are within error. Thus, as expected, obtained values of kd are insensitive to sample concentration.
The influence of IS on kinetics
The monomer/dimer equilibrium of the NTD at pH 6 is dependent on IS9. Steady-state fluorescence emission intensities show that at low IS NTDF is dimeric but at IS>200 mM NTDF is essentially monomeric (Supplementary Fig. S3). We measured ka and kd of NTDF in pH 6.0-buffered solutions of increasing IS. The results are shown in Fig. 4. Kinetic transients together with fits to the data are provided as Supplementary Figs S4 and S5. ka is virtually insensitive to salt. By contrast, kd increases substantially with increasing IS. In fluorescence transients of NTDF dissociation at low IS, that is, at IS<100 mM, a second kinetic phase emerges that fits to an exponential rise time, which is two- to fourfold slower than that of the main kinetic phase (Supplementary Fig. S5). This additional phase may originate from the presence of low levels of NTD oligomers. Clustering fluorophores in oligomeric NTDF can undergo resonance energy transfer (homo-Förster resonance energy transfer ) leading to quenching of fluorescence and may explain the occurrence of an additional kinetic phase at low IS. In support of this interpretation, higher-order NTD oligomers have previously been observed in light-scattering experiments carried out at pH 6 (ref. 7). However, their presence is controversial as they have not been detected in other experimental studies10,13.
From the rate constants ka and kd we calculated the equilibrium dissociation constant, Kd, which is found to be in the nanomolar range and strongly dependent on IS. Kd provides access to the free energy of NTDF dimerization, ΔG. All thermodynamic parameters are summarized in Supplementary Table S1. To test whether the observed changes of kd and ΔG resulted from charge screening, we applied the Debye–Hückel formalism in the analysis of the data, which describes the activity of ions in solution14. Following this law, a change of ΔG originating from charge screening is proportional to the square root of IS (IS0.5)15,16. We plotted ΔG of NTD assembly versus IS0.5 and found that it changed linearly, in accord with the Debye–Hückel law (Fig. 4, inset). From the linear fit to the data, we extrapolate the dissociation rate constant at zero IS, kd0, to 0.025±0.006 s−1. The extrapolated free energy at zero IS, ΔG0, is 13.8±0.2 kcal mol−1.
The influence of NTD side chain charges on kinetics
The 137-residue sequence of the NTD contains a total of 12 ionizable amino-acid side chains: eight negatively charged aspartic or glutamic acids (D or E), three positively charged arginine or lysine residues (R or K) and one histidine (H). To probe their contribution to assembly, we deleted the side chain charges individually on the background of NTDF using site-directed mutagenesis. Aspartic and glutamic acids were mutated to the isosteric, non-charged asparagines and glutamines. Histidine, lysine and arginine were mutated to alanine. Kinetic transients of association and dissociation of NTDF mutants were measured in 50 mM MES pH 6.0 using stopped-flow fluorescence spectroscopy. Representative transients are shown in Fig. 3a,d. Values of ka and kd were derived in the same way as described above for the non-mutated pseudo wild-type protein. Kinetic transients of all mutants had significant amplitudes, except of D39N and D40N. D39N and D40N had virtually no fluorescence signal change and, thus, no rate constants of association or dissociation could be obtained. The values of ka and kd determined for all other mutants are shown in Fig. 5. All rate constants together with derived thermodynamic data are provided in Supplementary Table S1. ka was between 1–5 × 108 M−1 s−1 and fluctuates around the value determined for the pseudo wild-type NTDF (Fig. 5a). Values of kd vary with position and are significantly elevated (up to 13-fold) for all mutants (Fig. 5b). Mutant E119Q is an exception as it shows a fivefold decrease of kd as compared with the pseudo wild-type protein.
Discussion
The NTD of spidroins evolved to maintain solubility of silk fibre-building blocks in the ampulla of the spinning gland and to assist their transformation into threads on demand5. The sequence composition of the NTD is unusual and its fold is unique with no homologue identified so far7. Notably, only 9% of NTD amino-acid side chains are ionizable, which is a small number compared with the average 29% in proteins17. This is in particular remarkable, as charged side chains on protein surfaces usually provide water solubility. The side chain charges of the NTD are very likely to have additional roles in spidroin assembly because a protonation event at physiological pH triggers NTD self-association in the spinning duct7,9,10. The mechanistic understanding of this process has two aspects: first, the functionality of the pH-sensitive relay that triggers association and, second, the actual association process itself.
NTDs dimerize promoted by a decrease of solution pH from 7 to 6 (refs 7, 9, 10) (Fig. 1). Thus, a protonation event acts on monomeric NTD where it triggers a change of structural and/or physicochemical properties that facilitates subsequent association. The site(s) of protonation in the NTD and the mechanism of the pH relay are not yet established. To lose or take a proton, the pKas of involved side chains must lie within the range of the applied pH change, that is, between 7 and 6. The normal pKa of carboxylic acids in proteins is 3–4 but strongly depends on the local environment17. Proximity of negatively charged carboxylates leads to unfavourable Coulomb repulsion and can elevate their pKas to physiological value, thus alleviating repulsive Coulomb forces through protonation17. Askarieh et al.7 suggested from the crystal structure of the dimer and mutagenesis experiments that a direct interaction between the D40 and E84 side chain carboxylates could generate a pH relay. However, a subsequent solution NMR study showed significant structural differences between monomeric and dimeric forms of the NTD13 (Fig. 6a). From the solution structure it is evident that the interaction between D40 and E84, which is present in the dimer, is absent in the monomer. This seems to exclude the D40/E84 interaction as a pH relay. Here we deleted all 12 side chain charges of the NTD individually and probed their influence on kinetics of dimerization. From our analysis, it emerges that D39N and D40N are the only mutants that abolish dimerization (Fig. 5). Both mutants had virtually no fluorescence signal change in rapid-mixing experiments (Fig. 3a). Mutation E84Q, on the other hand, had only a minor effect. The observed inability of D40N to form dimers is in agreement with results from Askarieh et al.7 as well as fluorescence and mass spectrometry studies7,18. However, the effect of mutating D39 was not examined in these previous studies. Results suggests that D39 and D40 may form a pH relay. The side chains of D39 and D40 are fixed on a helix in close spatial proximity, both in monomeric and dimeric NTD (Fig. 6a). D39 and D40 may be close enough to elevate their pKas to physiological value. A mutual pKa-elevating effect would be abolished on mutation of either one of the two side chains to asparagine and protonation-induced dimerization at pH 6 would be abrogated, as observed in our experiments. However, there is an additional side chain that may have a role. E119Q was the only mutation that significantly stabilizes the dimer. The dissociation rate constant of E119Q is fivefold slower than that of the pseudo wild-type protein and provides a stabilizing free energy of 0.7±0.2 kcal mol−1 (Fig. 5b). It is conceivable that an elevated side chain pKa of E119 leads to protonation at pH 6, thereby removing the charge of the carboxylate. In support of this interpretation, computer simulations predicted a destabilizing penetration of water molecules into the dimerization interface mediated by the E119 carboxylate19. However, experimental evidence for the protonation of specific side chains in the NTD at pH 6 is still incomplete and may eventually come from high-resolution spectroscopic techniques such as NMR17,20.
Other ionizable side chains also contribute to assembly. We find that mutation of R60 and K65 results in substantial destabilization of the dimer by free energy increments of ~2 kcal mol−1 (Fig. 5b). Positively charged R60 and K65 form intersubunit salt bridges with D39 and D40 (Fig. 6b). The stabilizing effect of these salt bridges has recently been addressed by computer simulations21. We also found that mutation of K93 destabilizes the dimer (Fig. 5b). However, this effect is difficult to interpret because the K93 side chain is in a buried position (Fig. 6c) and its mutation to alanine may possibly induce a structural perturbation. H6A and E79Q have values of ka and kd that are not substantially different from those of the pseudo wild-type protein. This corroborates findings from previous experimental studies where both mutants were still able to form dimers7,18.
The second question surrounding the mechanism of NTD assembly involves the actual association process itself that follows the protonation event(s). The universal theory of protein–protein association is well established. The shape of most proteins can be approximated as globules and their rates of random collision in aqueous solution is estimated from the Einstein–Smoluchowski equation to ~6 × 109M−1 s−1 at 25 °C (ref. 22). Protein association, however, rarely reaches this speed limit set by diffusion. Protein association has strict geometric constraints where complementary shapes require well-defined orientations before they can assemble. The probability of correct orientation of interfaces during a random collision is estimated to ~1.5 × 10−5 and results in the basal rate constant of protein–protein association of ~105 M−1 s−1 (refs 15, 22, 23). Here we observe an unusually high NTD association rate constant of 3 × 108 M−1 s−1, which is three orders of magnitude faster than the basal value and close to the diffusion speed limit. Favourable intermolecular Coulomb forces originating from opposite charges on protein interfaces can accelerate association to beyond the basal value15. This accelerating effect, however, is commonly screened by electrolytes in solution with numerous examples reported in the literature15,16,24,25,26,27,28. Surprisingly, we find that association of the NTD is insensitive to such screening (Fig. 4). But IS substantially accelerates NTD dissociation (Fig. 4). This behaviour is the exact opposite of what is commonly observed15. Findings are corroborated by results from mutagenesis: deletion of NTD side chain charges have virtually no influence on the rate constant of association but accelerate dissociation (Fig. 5a,b). Previous structural studies analysed the distribution of side chain charges on the surface of the NTD7. The authors found that opposite charges cluster on opposing poles of the association interface, thereby generating a unique macromolecular dipole7. The anti-parallel symmetry of the interface leads to a favourable dipole–dipole interaction (Fig. 6c). This dipole interaction may provide the alignment force for accelerated association and explain its unusual insensitivity to dielectric screening. Early simulations on cytochrome c peroxidase emphasized the importance of dipolar interactions as steering force in proteins29. Gilson et al.30 predicted from calculations of protein electrostatics that for many interactions involving dipoles, solvent screening can essentially be ignored. In line with this argument, Lockhart and Kim31 found experimental evidence that, in contrast to charges, helix dipole interactions in proteins are hardly screened by IS.
Findings may have implications in the synthesis of silk fibres by web spiders. For spiders, the speed of dragline synthesis is vital. The fibre is being used both as web frame and the spider’s lifeline. At a vertical fall and during roping, reeling speed can exceed 1 m s−1 (refs 1, 32). At such high reeling speed and rate of fibre synthesis, the residence time of spidroins in the acidified NTD assembly zone of the duct is only about 1 ms depending on spider size. Spidroin NTDs must therefore associate very quickly to assist silk synthesis. The spinning duct of web spiders contains up to 50% spidroin1. With a spidroin molecular weight of ~300 kDa, the concentration equals ~1 mM. Together with the basal rate constant of protein–protein association of ~105 M−1 s−1 (refs 15, 22, 23), the concentration yields a basal association time of ~10 ms, which is already too slow for rapid fibre synthesis. The association of the NTD in context of an intact spidroin in the spinning gland may even occur significantly slower than estimated from the basal rate constant of protein association. Although the NTD is an autonomously folded, structurally independent domain it takes only ~5% of a spidroin sequence. Its mobility could additionally be slowed by the presence of the extended repetitive domains that undergo structural transitions in the gland. Moreover, the high spidroin concentration leads to unusual viscid solution properties1 that will further slow diffusional motions of the NTDs. These considerations emphasize the requirement of accelerated NTD association beyond the basal value if it should assist the process of silk synthesis at high reeling speed. Acceleration by a common charge-driven process would be perturbed by the presence of physiological electrolytes in the spinning duct34. In the distal part of the duct where the silk fibre is formed, water is recovered from the spidroin solution1,4 leading to even an increase of IS. A macromolecular NTD dipole interaction that resists dielectric screening could accelerate association irrespective of IS, as observed in our experiments, and thus facilitate the synthesis of dragline silk at high reeling speed. Sequence conservation of NTDs from various silk glands and spider species suggests that such process could be a universal feature of silk fibre synthesis by web spiders.
Methods
Synthesis and mutagenesis and fluorescence modification of the NTD
The complementary DNA of the NTD from spidroin 1 of the major ampullate gland of the nursery web spider E. australis was purchased as synthetic gene, codon optimized for expression in Escherichia coli bacterial cells (GeneArt, Life Technologies). The NTD gene containing an N-terminal His6-tag followed by the thrombin recognition sequence (LVPRGS) for proteolytic cleavage was cloned into a modified pRSETA vector (Invitrogen). Single-point mutants were generated using the QuikChange mutagenesis protocol (Stratagene). NTD and mutants thereof were overexpressed in E. coli C41 (DE3) cells and isolated from clarified cell lysate by nickel nitrilotriacetic acid affinity chromatography, followed by proteolytic removal of the His6-tag using thrombin from bovine plasma (Sigma). The thrombin-digested eluate from affinity chromatography was dialyzed into 20 mM MES pH 6.0, subsequently applied to an anion-exchange chromatography column (Poros HQ resin equilibrated with 20 mM MES pH 6.0) and eluted with a gradient from 0 to 500 mM sodium chloride. Pooled NTD fractions were purified to homogeneity using size exclusion chromatograpy (SEC) on a Superdex 75 column (GE Healthcare) equilibrated with 200 mM ammonium bicarbonate. The pooled fractions from SEC were lyophilized and stored at −20 °C. NTD-Q50C and mutants thereof were modified with the thiol-reactive maleimide derivative of the oxazine fluorophore AttoOxa11 (Atto-Tec). Labelling was carried out using a 10-fold molar excess of fluorophore reacted for 2.5 h at 25 °C in 50 mM 3-(morpholino)propanesulphonic acid, pH 7.5, containing 6 M guanidinium chloride and a 10-fold molar excess of tris(2-carboxyethyl)phosphine (Sigma) to prevent thiol oxidation. Labelled protein was isolated from unreacted dye using SEC.
Steady-state and time-resolved fluorescence experiments
Steady-state absorption and fluorescence emission spectra were recorded on a V-650 spectrophotometer and a FP-6500 spectrofluorimeter (Jasco). Association and dissociation kinetics were measured using stopped-flow fluorescence spectroscopy. Trp fluorescence data of NTD were recorded on an Applied Photophysics (SX 18MV) machine equipped with a xenon-lamp as excitation source. AttoOxa11 fluorescence data of NTDF were recorded on a SFM-2000 machine (BioLogic Instruments) using a diode at 639 nm as excitation source. Samples were filtered through 0.2 μm syringe filters before measurement. For determination of ka of NTD, NTDF and mutants thereof, protein samples were prepared at varying concentration in 10 mM MES pH 7.0 with IS adjusted to 150 mM and rapidly mixed into 50 mM MES buffer at pH 6.0 using a volumetric mixing ratio of 1:10. For measurement of kd of NTDF and mutants thereof, 100 nM samples were prepared in 50 mM MES pH 6.0 and chased with 5 μM unlabelled NTD prepared in the same buffer, using a volumetric mixing ratio of 1:10. IS of buffers was adjusted using potassium chloride. All measurements were done at 25 °C.
Data analysis
Kinetic transients were fitted to exponential decay and rise functions. Values of ka of NTD, NTDF and mutants thereof were determined from the slopes of linear data fits to the concentration dependencies of kobs (five different concentrations each). Values of kd from chasing experiments were determined as average values of observed rate constants from three measurements. The equilibrium dissociation constant, Kd, and the free energy of dimerization, ΔG, was calculated from ka and kd:
R is the gas constant and T the temperature (298 K). The change of free energy on mutation, ΔΔG, was calculated by:
where KdWT and KdM are the Kd values of NTDF (WT) and a single-point mutant (M).
Additional information
How to cite this article: Schwarze, S. et al. The N-terminal domains of spider silk proteins assemble ultrafast and protected from charge screening. Nat. Commun. 4:2815 doi: 10.1038/ncomms3815 (2013).
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Acknowledgements
We thank the Deutsche Forschungsgemeinschaft (DFG) for financial support (Grant number NE 1201/3-1).
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H.N. designed research; S.S., F.U.Z., C.M.J. and H.N. performed research; S.S., C.M.J. and H.N. analysed data; H.N. wrote the paper.
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Schwarze, S., Zwettler, F., Johnson, C. et al. The N-terminal domains of spider silk proteins assemble ultrafast and protected from charge screening. Nat Commun 4, 2815 (2013). https://doi.org/10.1038/ncomms3815
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DOI: https://doi.org/10.1038/ncomms3815
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NMR assignments of a dynamically perturbed and dimerization inhibited N-terminal domain variant of a spider silk protein from E. australis
Biomolecular NMR Assignments (2020)
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The transcriptome of Darwin’s bark spider silk glands predicts proteins contributing to dragline silk toughness
Communications Biology (2019)
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