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Mirror-design of L-oligonucleotide ligands binding to L-arginine

Nature Biotechnology volume 14, pages 1116–1119 (1996)Cite this article

Abstract

The high affinity and selectivity of nucleic acid ligands have clearly demonstrated that RNA can be targeted to a variety of molecules. In practice, however, the use of unmodified aptamers is impeded by the low stability of RNA in biological fluids. Here we describe the mirror-design of a stable 38-mer L-oligoribonucleotide ligand that binds to L-arginine. This L-RNA ligand was also able to bind to a short peptide containing the basic region of the human immunodeficiency virus type-1 Tat-protein. The L-RNA ligand displayed the expected stability in human serum. These findings may contribute to the identification of novel diagnostics and pharmaceuticals.

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Author notes

  1. Jens P. Fürste: fuerste@chemie.fu-berlin.de

Authors and Affiliations

  1. Institut für Biochemie, Freie Universität Berlin, Thielallee 63, D-14195, Berlin, Germany

    Alexis Nolte, Sven Klußmann, Rolf Bald, Volker A. Erdmann & Jens P. Fürste

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  1. Alexis Nolte
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  2. Sven Klußmann
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  3. Rolf Bald
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  4. Volker A. Erdmann
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  5. Jens P. Fürste
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Nolte, A., Klußmann, S., Bald, R. et al. Mirror-design of L-oligonucleotide ligands binding to L-arginine. Nat Biotechnol 14, 1116–1119 (1996). https://doi.org/10.1038/nbt0996-1116

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