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PTPIP51 interaction with PTP1B and 14-3-3β in adipose tissue of insulin-resistant mice

International Journal of Obesity volume 35, pages 1385–1394 (2011)Cite this article

Subjects

Abstract

Objective:

We investigated the expression of protein tyrosine phosphatase-interacting protein 51 (PTPIP51) and its interaction with protein tyrosine phosphatase 1B (PTP1B) and 14-3-3β in mice exhibiting insulin resistance and obesity.

Design:

A total of 20 mice were included in the study. Eight control animals were fed a normal standard diet, six animals were fed a high-fat diet and six animals were submitted to a treadmill training parallel to the feeding of a high-fat diet. After 10 weeks, a glucose tolerance test was performed and abdominal adipose tissue samples of the animals were collected.

Results:

PTPIP51 protein was identified in the adipocytes of all samples. PTPIP51 interacted with PTP1B and with 14-3-3β protein. Compared with untrained mice fed a standard diet, the interaction of PTPIP51 with PTP1B was reduced in high-fat diet-fed animals. The highest interaction of PTPIP51 with 14-3-3β was seen in trained animals on high-fat diet, whereas untrained animals on high-fat diet displayed lowest values.

Conclusion:

PTPIP51 is expressed in adipose tissue of humans, rats and mice. Obesity with enhanced insulin resistance resulted in a reduction of PTPIP51 levels in adipocytes and influenced the interactions with PTP1B and 14-3-3β. The interaction of PTPIP51 with PTP1B suggests a regulatory function of PTPIP51 in insulin receptor signal transduction. The interaction of PTPIP51 with 14-3-3β, especially in trained individuals, hints to an involvement of PTPIP51 in the downstream regulation of insulin action.

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Acknowledgements

We are grateful to Ms A Erkel and Ms B Fröhlich (Department of Urology and Paediatric Urology, Giessen, Germany) for their excellent technical assistance.

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Authors and Affiliations

  1. Institute of Anatomy and Cell Biology, Justus-Liebig-University, Giessen, Germany

    M Bobrich, A Brobeil, C Tag & M Wimmer

  2. Department of Sports Medicine, Justus-Liebig-University, Giessen, Germany

    F C Mooren & K Krüger

  3. Department of Urology and Paediatric Urology, Justus-Liebig-University Giessen, Giessen, Germany

    K Steger

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  1. M Bobrich
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Correspondence to M Bobrich.

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Bobrich, M., Brobeil, A., Mooren, F. et al. PTPIP51 interaction with PTP1B and 14-3-3β in adipose tissue of insulin-resistant mice. Int J Obes 35, 1385–1394 (2011). https://doi.org/10.1038/ijo.2010.283

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