Abstract
THYMUS-DERIVED lymphocytes (T) have been shown to have a major role in cell-mediated immunity against foreign transplants and syngeneic tumours1,2. In vitro, several assays have been developed to measure thymus-dependent cell-mediated immunity, making use of the cytotoxic potential of these effector cells3. Precise estimates of the number of cytotoxic T lymphocytes (CTL), however, are not possible by available methods. We report here a new method to quantify the CTL present in a mixed cell population. The method is based on the ability of CTL to kill specifically adjacent target cells fixed on monolayers and to form zones of lysis or plaques.
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References
Cerottini, J.-C., and Brunner, K. T., Adv. Immun., 18, 67–132 (1974),
Herberman, R. B., et al., Prog. Immun., 3, 285–295 (1974).
Bloom, B. R., and Glade, P. R., In Vitro Methods in Cell-Mediated Immunity (Academic, New York, 1971).
Kedar, E., Ortiz de Landazuri, M., Bonavida, B., and Fahey, J. L., J. Immun. Meth., 5, 97–102 (1974).
Martz, E., J. Immun., 115, 261–267 (1975).
Berke, G., Gabeson, D., and Feldman, Eur. J. Immun., 5, 813–181 (1975).
Wilson, D. B., Blyth, J. L., and Nowell, P. C., J. exp. Med., 128. 1157–1181 (1968).
Nisbet, N. W., Simonsen, M., and Zaleski, M., J. exp. Med., 129, 459–467 (1969).
Binz, H., and Wigzell, H., J. exp. Med., 142, 1218–1230 (1975).
Ford, W. L., Simmonds, S. F., and Atkins, R. C., J. exp. Med., 141, 681–696 (1975).
Bonavida, B., J. Immun., 112, 1308–1321 (1974).
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BONAVIDA, B., IKEJIRI, B. & KEDAR, E. Direct estimation of frequency of cytotoxic T lymphocytes by a modified plaque assay. Nature 263, 769–771 (1976). https://doi.org/10.1038/263769a0
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DOI: https://doi.org/10.1038/263769a0
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