Abstract
A chemically synthesised duplex DNA fragment containing the sequence of the lac operator was cloned in E. coli using the vehicle pMB9. Clones containing lac-pMB9 hybrid DNA produced β-galactosidase constitutively and the hybrid DNA bound the lac repressor specifically.
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Marians, K., Wu, R., Stawinski, J. et al. Cloned synthetic lac operator DNA is biologically active. Nature 263, 744–748 (1976). https://doi.org/10.1038/263744a0
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DOI: https://doi.org/10.1038/263744a0
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