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Detection of slow rotational motions of proteins by steady-state phosphorescence anisotropy

Abstract

WE have used the anisotropy of the phosphorescence from intrinsic chromophores of proteins to detect slow rotational motions of the macromolecules in highly viscous solutions. This method has potential for monitoring the mobility of proteins in structures such as membranes and viruses.

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STRAMBINI, G., GALLEY, W. Detection of slow rotational motions of proteins by steady-state phosphorescence anisotropy. Nature 260, 554–556 (1976). https://doi.org/10.1038/260554a0

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