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JNK activation is not required for Fas-mediated apoptosis

Oncogene volume 18, pages 3737–3741 (1999)Cite this article

Abstract

Fas ligation in the presence of cycloheximide induced Jun N-terminal kinase 1 (JNK1) and JNK2 phosphorylation, caspase activation and cell death in the IL-3-dependent cell line BAF3. Fas-mediated apoptosis was prevented by expression of dominant negative FADD but not inhibited by IL-3. To investigate the role of JNK activation in this process, we examined cells over-expressing a JNK-specific phosphatase M3/6. M3/6 prevented Fas stimulation of JNK, but did not affect Fas-mediated caspase activation or cell death, demonstrating that JNK activation is not required for these processes.

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Acknowledgements

This work was supported by the Cancer Research Campaign and by a Wellcome Trust Travelling Fellowship to Walter Low.

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Authors and Affiliations

  1. Department of Immunology, University College London, Windeyer Building, 46 Cleveland Street, London, W1P 6DB, UK

    Walter Low & Mary Collins

  2. Section of Gene Function and Regulation, Chester Beatty Laboratories, Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, UK

    Anna Smith & Alan Ashworth

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Low, W., Smith, A., Ashworth, A. et al. JNK activation is not required for Fas-mediated apoptosis. Oncogene 18, 3737–3741 (1999). https://doi.org/10.1038/sj.onc.1202702

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