Nature Methods - Application Notes
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Noninvasive, in vivo quantification of asthma severity using fluorescence molecular tomography
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VisEn Medical has developed FMT™ 2500, a quantitative fluorescence molecular tomographic system useful for rapid, noninvasive measurement of disease biology in living animals. This imaging technology, paired with one of VisEn's protease-activatable agents, ProSense® 680, has enabled the quantification of disease-related protease activity in the mouse ovalbumin-induced model of asthma.
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Sartorius Stedim
SuperSpinner D 1000: a disposable bioreactor for efficient lab-scale cultivation of animal cells
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Sufficient oxygen supply is essential for proper function of all energy-consuming processes during the cell cycle. Oxygen limitation results in slow growth and production kinetics. Effective aeration during cell cultivation is one requirement to achieve high cell densities and high product concentrations. The SuperSpinner D 1000 is a disposable bioreactor that ensures optimal oxygen supply and high productivity during the cultivation of animal cells in a scale of 1 liter.
Carestream Molecular Imaging
Refining epifluorescence imaging and analysis with automated multiple-band flat-field correction
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The new KODAK In-Vivo Multispectral Imaging System FX uses an innovative calibration tool, the Kodak Epi Field Flattening Screen, which provides efficient calibration over a wide range of excitation and emission filter combinations. When used with Kodak MI v5.0, software that automates collection, calibration and analysis of multimodal images both in vivo and in vitro, the screen enables accurate fluorescence measurement of samples placed anywhere on the imaging surface, improving quantitative accuracy and overall throughput.
ProFACT Proteomics, Inc.
Molecular Profiling With SeraFILE
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Proteins are the principal active engines of biology. Since proteins are subject to post-translational modifications, protein-protein interactions, and changes in expression patterns, proteomics applications are more comprehensive in scope than genomics. For these reasons, protein science may shed more light on cellular responses to environmental changes and stress conditions than DNA-based applications.
Olympus
Visualizing axon guidance phenotypes induced by RNAi in chicken embryos
The ability to turn off a gene of interest in a temporally and spatially controlled manner is key to the analysis of gene function in developing organisms. However, the induction of temporally controlled loss-of-function phenotype is very difficult. A combination of RNA interference (RNAi) with fluorescence microscopy analysis is proving to be a very good solution to this problem. Here we show that the Olympus SZX stereo and upright BX microscopes, both with fluorescence illumination capabilities, are perfect for use in these situations.
EMD
Comparing the Speed and Product Yield of 7 high fidelity DNA Polymerases
Thermococcus kodakaraensis (strain KOD1), previously thought to be a Pyrococcus sp., is a hyperthermophilic archaea isolated from a solfataric hot spring on Kodakara Island, Japan (Atomi 2004). In preliminary studies to characterize the KOD1 DNA polymerase, researchers found that the enzyme had fidelity comparable to Pfu DNA polymerase, but with an extension rate (referred to as speed) 5 times higher and processivity 10 to 15 times higher than the Pfu enzyme (Takagi 1997). When amplifying DNA for cloning, high fidelity DNA polymerases, such as KOD polymerase, are recommended.
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