Featured
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Article
| Open Access
StayGold variants for molecular fusion and membrane-targeting applications
Monomeric and tandem dimer derivatives of the bright and photostable green fluorescent protein StayGold offer versatile tools for tagging target proteins and membranes in extended live-cell imaging.
- Ryoko Ando
- , Satoshi Shimozono
- & Atsushi Miyawaki
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Research Briefing |
Capturing detailed cellular landscapes by montage cryo-electron tomography
To capture expansive, seamless fields of view from frozen hydrated specimens by cryo-electron tomography, we developed methods for the collection and processing of montage data. This approach enables rapid acquisition of contiguous regions of specimens using a montaged tilt series collection scheme.
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Brief Communication |
An optical design enabling lightweight and large field-of-view head-mounted microscopes
Two miniature microscopes with innovative light paths are described and applied to imaging of juvenile zebra finches and mice.
- Joseph R. Scherrer
- , Galen F. Lynch
- & Michale S. Fee
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Article |
Cell region fingerprints enable highly precise single-cell tracking and lineage reconstruction
TracX improves the accuracy of single-cell tracking by using a fingerprinting approach to measure the similarity between cells in two consecutive images. The approach is applicable across modalities and enables biological discovery.
- Andreas P. Cuny
- , Aaron Ponti
- & Jörg Stelling
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Brief Communication
| Open Access
Micro-Meta App: an interactive tool for collecting microscopy metadata based on community specifications
Micro-Meta App is an intuitive, highly interoperable, open-source software tool designed to facilitate the extraction and collection of relevant microscopy metadata as specified by recent community guidelines.
- Alessandro Rigano
- , Shannon Ehmsen
- & Caterina Strambio-De-Castillia
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Article |
Universal autofocus for quantitative volumetric microscopy of whole mouse brains
RAPID (rapid autofocusing via pupil-split image phase detection) is a sample-agnostic real-time autofocus method for widefield microscopy. RAPID removes most image degradation in large, cleared samples for enhanced quantitative analyses.
- L. Silvestri
- , M. C. Müllenbroich
- & F. S. Pavone
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Research Highlight |
Breaking barriers for intravital imaging
Advances in adaptive optics combined with a twist on light field microscopy enable high-quality imaging.
- Rita Strack
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Brief Communication |
Molecular-scale axial localization by repetitive optical selective exposure
ROSE-Z achieves axial interference through an asymmetrical optical scheme, yielding 2 nm axial localization precision with ~3,000 photons and a single objective, which offers improved multicolor three-dimensional localization microscopy for cellular structures.
- Lusheng Gu
- , Yuanyuan Li
- & Wei Ji
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Article |
Three-dimensional virtual refocusing of fluorescence microscopy images using deep learning
Deep-Z uses deep learning to go from a two-dimensional snapshot to three-dimensional fluorescence images. The method improves imaging speed while reducing light dose, and was shown to be useful for accurate structural and functional imaging of neurons in Caenorhabditis elegans.
- Yichen Wu
- , Yair Rivenson
- & Aydogan Ozcan
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Article |
Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits
Simultaneous two-photon microscopic and one-photon mesoscopic imaging of calcium signals enables correlation of local cellular and brain-wide network activity.
- Daniel Barson
- , Ali S. Hamodi
- & Michael J. Higley
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Article |
Nuclear pores as versatile reference standards for quantitative superresolution microscopy
Cell lines in which Nup96 is endogenously tagged with mEGFP, SNAP-tag, HaloTag or mMaple serve as versatile reference samples, enabling 3D resolution calibration, assessment of labeling efficiency and precise molecular counting.
- Jervis Vermal Thevathasan
- , Maurice Kahnwald
- & Jonas Ries
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Perspective |
Applications, promises, and pitfalls of deep learning for fluorescence image reconstruction
This Perspective highlights recent applications of deep learning in fluorescence microscopy image reconstruction and discusses future directions and limitations of these approaches.
- Chinmay Belthangady
- & Loic A. Royer
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Research Highlight |
A tiny macroscope to look into complex behavior
A macroscope mounted on the head of a freely moving transgenic rat can help shine light on complex behaviors.
- Alfredo Sansone
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Brief Communication |
Optogenetic control with a photocleavable protein, PhoCl
A photocleavable protein (PhoCl) that spontaneously dissociates into two fragments after illumination with violet light expands the toolkit for cellular optogenetics.
- Wei Zhang
- , Alexander W Lohman
- & Robert E Campbell
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Brief Communication |
Cryogenic optical localization provides 3D protein structure data with Angstrom resolution
The positions of fluorophores can be localized in single proteins with Angstrom-scale resolution using Cryogenic Optical Localization in 3D (COLD), a complementary approach to traditional structural biology techniques.
- Siegfried Weisenburger
- , Daniel Boening
- & Vahid Sandoghdar
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Article |
A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein
A bright and photostable far-red fluorescent protein, smURFP, was developed from a cyanobacterial phycobiliprotein. smURFP uniquely binds a highly cell-permeable biliverdin derivative to obtain fluorescence brightness comparable to that of eGFP in cells.
- Erik A Rodriguez
- , Geraldine N Tran
- & Roger Y Tsien
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Brief Communication |
siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data
Single-image fluorescence lifetime imaging microscopy (siFLIM) uses a modulated-FLIM camera to record lifetimes in a single snapshot, allowing for photon-efficient and quantitative lifetime imaging of rapid cellular processes.
- Marcel Raspe
- , Katarzyna M Kedziora
- & Kees Jalink
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Brief Communication |
Expansion microscopy with conventional antibodies and fluorescent proteins
Improved sample preparation methods allow super-resolution imaging by expansion microscopy using endogenous fluorescent protein signal and conventional fluorescently labeled antibodies.
- Tyler J Chozinski
- , Aaron R Halpern
- & Joshua C Vaughan
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Brief Communication |
Ultrahigh-throughput single-molecule spectroscopy and spectrally resolved super-resolution microscopy
Spectrally resolved STORM (stochastic optical reconstruction microscopy) uses wide-field spectral measurements of sparsely activated single-molecule emitters to image cells labeled with fluorophores with highly overlapping emission spectra, opening the door to multiplexed super-resolution imaging.
- Zhengyang Zhang
- , Samuel J Kenny
- & Ke Xu
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Tools in Brief |
A multifocal approach to whole-cell super-resolution imaging
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Correspondence |
A simple image correction method for high-throughput microscopy
- Adam D Coster
- , Chonlarat Wichaidit
- & Lani F Wu
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Research Highlights |
Attention to detail
Computational processing allows sub–diffraction-limit resolution imaging with a standard wide-field microscope.
- Michael Eisenstein
Pretraining a foundation model for generalizable fluorescence microscopy-based image restoration