To capture expansive, seamless fields of view from frozen hydrated specimens by cryo-electron tomography, we developed methods for the collection and processing of montage data. This approach enables rapid acquisition of contiguous regions of specimens using a montaged tilt series collection scheme.
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References
Turk, M. & Baumeister, W. The promise and the challenges of cryo-electron tomography. FEBS Lett. 594, 3243–3261 (2020). A review summarizing the emergence of cryo-ET in structural biology, recent technology developments and future directions.
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Peck, A. et al. Montage electron tomography of vitrified specimens. J. Struct. Biol. 214, 107860 (2022). This paper experimented with montage cryo-ET to obtain a single montage tilt series using very restricted beam size to support an efficient hexagonal tiling pattern, which will minimize radiation damage.
Eisenstein, F. et al. Parallel cryo electron tomography on in situ lamellae. Nat. Methods 20, 131–138 (2023). This paper presents a method for collecting tilt series in parallel via an image shift and geometry-based sample model and iteratively refined tracking and autofocus.
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This is a summary of: Yang, J. E. et al. Correlative montage parallel array cryo-tomography for in situ structural cell biology. Nat. Methods https://doi.org/10.1038/s41592-023-01999-5 (2023).
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Capturing detailed cellular landscapes by montage cryo-electron tomography. Nat Methods 20, 1458–1459 (2023). https://doi.org/10.1038/s41592-023-02000-z
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DOI: https://doi.org/10.1038/s41592-023-02000-z
