Article
|
Open Access
Featured
-
-
Article
| Open Access
Wheat Pm55 alleles exhibit distinct interactions with an inhibitor to cause different powdery mildew resistance
Powdery mildew threatens worldwide wheat production. Here, the authors report the cloning of two powdery mildew resistant Pm55 alleles and show that they exhibit distinct interactions with the inhibitor SuPm55 to cause different resistance.
- Chuntian Lu
- , Jie Du
- & Ruiqi Zhang
-
Article
| Open Access
Sequencing trait-associated mutations to clone wheat rust-resistance gene YrNAM
Wheat stripe (yellow) rust is a devastating disease posing a threat to global production. Here, the authors report the cloning of a wheat stripe rust resistance gene encoding a protein with a NAM domain and a ZnF-BED domain using a strategy called sequencing trait-associated mutations (STAM).
- Fei Ni
- , Yanyan Zheng
- & Jiajie Wu
-
Article
| Open Access
Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar
Modifying plant lignin pathway to enhance saccharification efficiency is often associated with growth penalty. Here, the authors show that overexpression of Pag-miR408 in poplar leads to enhanced saccharification efficiency and growth in both laboratory and field conditions, and laccase genes are the targets of Pag-miR408.
- Yayu Guo
- , Shufang Wang
- & Jinxing Lin
-
Article
| Open Access
POWR1 is a domestication gene pleiotropically regulating seed quality and yield in soybean
In soybean production, level of seed protein frequently shows a negative correlation with seed oil content and yield. Here, the authors report a CCT gene pleiotropically regulates these traits and the selection of larger seeds determining allele leads to higher oil content but lower protein content in soybean cultivars.
- Wolfgang Goettel
- , Hengyou Zhang
- & Yong-qiang Charles An
-
Article
| Open Access
RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis
Unlike insects and mice, CRISPR/Cas9-based gene drives have not been achieved in plants. Here, the authors demonstrate homozygous F1 Arabidopsis plants can be obtained through zygotic conversion using CRISPR/Cas9-mediated homology-directed repair.
- Tao Zhang
- , Michael Mudgett
- & Yunde Zhao
-
Article
| Open Access
A Bacillus thuringiensis Cry protein controls soybean cyst nematode in transgenic soybean plants
Genetic resistance to soybean cyst nematode (SCN) is beginning to decline. Here, the authors report that soybean lines expressing the Bacillus thuringiensis protein Cy14Ab have significant levels of protection from SCN in both greenhouse and field trials.
- Theodore W. Kahn
- , Nicholas B. Duck
- & Julia Daum
-
Article
| Open Access
A serine/threonine protein kinase encoding gene KERNEL NUMBER PER ROW6 regulates maize grain yield
Selection of kernel number per ear has improved maize yield, but the genetic base is unclear. Here, the authors reveal that a serine/threonine protein kinase KNR6 is a positive regulator of the trait and show in vitro evidences that KNR6 may function through phosphorylating an Arf GTPase-activating protein.
- Haitao Jia
- , Manfei Li
- & Zuxin Zhang
-
Article
| Open Access
The indica nitrate reductase gene OsNR2 allele enhances rice yield potential and nitrogen use efficiency
Indica rice has higher nitrate assimilation and nitrogen use efficiency (NUE) than japonica rice, but the mechanism is unclear. Here, the authors reveal that the difference is partly due to allelic variation of a nitrate reductase encoding gene and this indica allele can increase yield potential and NUE.
- Zhenyu Gao
- , Yufeng Wang
- & Qian Qian
-
Article
| Open Access
Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems
Few approaches for targeted manipulation of the epigenome are available in plants. Here, the authors adapt the dCas9-SunTag system to engineer targeted gene activation and site-specific manipulation of DNA methylation in Arabidopsis.
- Ashot Papikian
- , Wanlu Liu
- & Steven E. Jacobsen
-
Article
| Open Access
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
Protocols for crop genome editing would ideally be quick, efficient and specific while avoiding integration of transgenes into the genome of edited plants. Here, Lianget al. show that CRISPR/Cas9 ribonucleoproteins can be used to generate genome edited wheat plants in as little as nine weeks.
- Zhen Liang
- , Kunling Chen
- & Caixia Gao
-
Article
| Open Access
Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA
Plant genome editing typically relies upon transgenic intermediates, which is a concern given the current regulatory requirements concerning GMOs. Here, Zhang et al. describe a method to edit wheat genomes by transiently expressing CRISPR/Cas9 DNA or RNA, and are able to generate mutant plants with no detectable transgenes.
- Yi Zhang
- , Zhen Liang
- & Caixia Gao
-
Article
| Open Access
A transgenic approach for controlling Lygus in cotton
Plant-feeding insects of the Lygus genus have emerged as a major pest effecting cotton crops in the USA. Here the authors optimize the insecticidal activity of a Bacillus thuringiensis crystal protein and produce transgenic plants that are resistant to feeding damage by Lygusspecies.
- Anilkumar Gowda
- , Timothy J. Rydel
- & James A. Baum
-
Article
| Open Access
Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase
The efficiency of cellulosic biofuel production from woody biomass is limited by the presence of lignin that impedes efficient processing. Here the authors show that transgenic modification of aspen to depress lignin polymerization can increase ethanol yield without affecting tree growth.
- Yuanheng Cai
- , Kewei Zhang
- & Chang-Jun Liu
-
Article |
Production of α-L-iduronidase in maize for the potential treatment of a human lysosomal storage disease
The lysosomal storage disease mucopolysaccharidosis I is treated with recombinant α-L-iduronidase but production of the enzyme is expensive. In this study, α-L-iduronidase is compartmentalized within the endosperm of maize via a unique mRNA strategy yielding the active, correctly glycosylated protein.
- Xu He
- , Thomas Haselhorst
- & Allison R. Kermode
A pair of atypical NLR-encoding genes confers Asian soybean rust resistance in soybean