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A cooperative nano-CRISPR scaffold potentiates immunotherapy via activation of tumour-intrinsic pyroptosis
Delivery of immune therapy drugs to tumours might be hampered by their limited bioavailability and the difficulty of targeting complex exogenous compounds. Here authors trigger immunologic cell death, via activating tumour-cell-intrinsic pathways via CRISPR-based nanotechnology to enable efficient anti-tumour immune response in mouse models of melanoma.
- Ning Wang
- , Chao Liu
- & Changyang Gong
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Article
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Multi-step screening of DNA/lipid nanoparticles and co-delivery with siRNA to enhance and prolong gene expression
Plasmid DNA offers extended transgene expression duration compared to mRNA technologies. Here, using a multi-step screening platform, the authors report the best performing nanoparticle formulations for liver-targeted plasmid DNA expression in vivo.
- Yining Zhu
- , Ruochen Shen
- & Hai-Quan Mao
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Article
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Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells
Ballistic delivery with micro/nano-particles has been successfully used to transfect plant cells, however, has failed in mammalian cells due to toxic effects. Here, the authors report on a self-assembled nano-ballistic delivery system for the delivery of functional macromolecules and demonstrate efficient transfection of mammalian cells.
- Juan C. Fraire
- , Elnaz Shaabani
- & Kevin Braeckmans
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Article
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An anionic, endosome-escaping polymer to potentiate intracellular delivery of cationic peptides, biomacromolecules, and nanoparticles
Most reagents designed to deliver cargo into cells are cationic and so cannot deliver cationic cargo. Here the authors show that pretreating cells with the anionic polymer poly(propylacrylic acid) facilitates the uptake and endosomal escape of a wide variety of cationic cargo in numerous cell types.
- Brian C. Evans
- , R. Brock Fletcher
- & Craig L. Duvall
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A RNA producing DNA hydrogel as a platform for a high performance RNA interference system
Interfering RNA have a range of therapeutic and research based applications, issues with delivery have made systems that make siRNA in situ of interest. Here, the author report on the creation of a DNA hydrogel with improved stability and transcription efficiency over plasmid DNA.
- Jaejung Song
- , Minhyuk Lee
- & Nokyoung Park
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Controlled gene and drug release from a liposomal delivery platform triggered by X-ray radiation
X-ray radiation has excellent tissue penetration depth, making it a useful trigger for deep tissue cancer therapy. Here, the authors design X-ray triggered drug/gene-loaded liposomes by embedding photosensitizers and gold nanoparticles in the liposome bilayer, and demonstrate their efficacy in cancer and gene therapy.
- Wei Deng
- , Wenjie Chen
- & Ewa M. Goldys
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Article
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Immunogene therapy with fusogenic nanoparticles modulates macrophage response to Staphylococcus aureus
In the context of increasing bacterial antibiotic-resistance, gene therapy that targets the immune system to clear infection is a major goal. Here the authors show a silicon based nanosystem that modulates the macrophage response in an in vivo model of Staphylococcal pneumonia.
- Byungji Kim
- , Hong-Bo Pang
- & Michael J. Sailor
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Metal-organic frameworks for precise inclusion of single-stranded DNA and transfection in immune cells
Non-viral vectors are important for transfection but can be limited in the uptake, protection and release of ssDNA. Here, the authors report on the design of metal-organic-framework vectors with precisely controlled pore geometry and demonstrate the vector in the transfection of immune cells.
- Shuang Peng
- , Binglin Bie
- & Xiang Zhou
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A fluorinated dendrimer achieves excellent gene transfection efficacy at extremely low nitrogen to phosphorus ratios
Polymers represent promising gene vectors due to their high efficiency and low cytotoxicity. Here, the authors show that fluorination increases gene transfection efficacy, while reducing cytotoxicity, and suggest an important role for this strategy in the design of efficient gene vectors.
- Mingming Wang
- , Hongmei Liu
- & Yiyun Cheng
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Article
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High-performance and site-directed in utero electroporation by a triple-electrode probe
In uteroelectroporation allows the labelling of specific populations of neurons in the developing mouse brain. The authors of this paper present a simple modification of this method that increases labelling efficiency and allows, for the first time, transfection of Purkinje cells in the rat cerebellum.
- Marco dal Maschio
- , Diego Ghezzi
- & Laura Cancedda
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A plasmid-based multigene expression system for mammalian cells
Fluorescent proteins are widely used in molecular biology to visualize protein expression and localization. Here, Krizet al. describe an efficient and flexible modular plasmid-based eukaryotic expression cloning strategy for the homogeneous expression of several fluorescent proteins in one cell.
- Andrijana Kriz
- , Katharina Schmid
- & Philipp Berger
High throughput intracellular delivery by viscoelastic mechanoporation