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| Open AccessUltrasound-activated piezo-hot carriers trigger tandem catalysis coordinating cuproptosis-like bacterial death against implant infections
Implantation-associated infections often lead to infections. Here, the authors propose a piezo-based nanoreactor to achieve US-excited tandem catalysis, endowing the polyetherketoneketone bone scaffold with on-demand antibacterial and osteogenic capacities.
- Yanli Huang
- , Xufeng Wan
- & Duan Wang
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Article
| Open AccessCultured meat with enriched organoleptic properties by regulating cell differentiation
Achieving organoleptic properties of conventional meat is important for cultured meat production. Here, the authors demonstrate that the texture, flavor, and nutritional content of cultured meat can be significantly enhanced by regulating cell differentiation inside hydrogel scaffolds.
- Milae Lee
- , Sohyeon Park
- & Jinkee Hong
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Article
| Open AccessFour-dimensional hydrogel dressing adaptable to the urethral microenvironment for scarless urethral reconstruction
Urethral repair can be carried out using hydrogels, but the harsh microenvironment hinders the repair. Here, the authors report the development of a 4D hydrogel dressing that can provide an early-vascularised and later-antifibrogenic microenvironment to assist in scarless reconstruction.
- Yujie Hua
- , Kai Wang
- & Kaile Zhang
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Article
| Open AccessTargeted mechanical stimulation via magnetic nanoparticles guides in vitro tissue development
Highly localized mechanical forces that shape in vivo tissue development remain challenging to recapitulate in vitro. Here the authors use magnetically actuated nanoparticles to generate spatially defined forces within organoids, which guide the spatial organization of tissue patterning and growth.
- Abdel Rahman Abdel Fattah
- , Niko Kolaitis
- & Adrian Ranga
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| Open AccessCultured meat platform developed through the structuring of edible microcarrier-derived microtissues with oleogel-based fat substitute
Cultured meat (CM) is a sustainable alternative for meat, providing similar eating experience and nutrition. Here the authors present and fully characterize a CM platform from cellularized edible microcarriers and oleogel-based fat substitute.
- Feng-Chun Yen
- , Jovana Glusac
- & Marcelle Machluf
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Article
| Open AccessEngraftment of allogeneic iPS cell-derived cartilage organoid in a primate model of articular cartilage defect
Allogeneic iPSC-derived cartilage organoids survive and integrate with surrounding native cartilage without immune reactions in a primate model of chondral defects in the knee joints, being remodeled and functioning as articular cartilage.
- Kengo Abe
- , Akihiro Yamashita
- & Noriyuki Tsumaki
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| Open AccessKidney epithelial cells are active mechano-biological fluid pumps
How mechanical forces drive fluid transport in the kidney remains unclear. Here, the authors use a microfluidic platform to show that kidney epithelial cells generate hydraulic pressure gradients across the epithelium, and that the fluid flux is from apical to basal for normal cells, and inverted in autosomal dominant polycystic kidney disease cells.
- Mohammad Ikbal Choudhury
- , Yizeng Li
- & Sean X. Sun
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Article
| Open AccessMeasuring mechanical anisotropy of the cornea with Brillouin microscopy
Here, Brillouin optical microscopy noninvasively visualizes microscale anisotropy of the porcine cornea owing to its lamellar fiber structure and quantifies the longitudinal moduli of the bulk tissue. Anisotropy is also detected in angle-resolved measurement of the human cornea in vivo.
- Amira M. Eltony
- , Peng Shao
- & Seok-Hyun Yun
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Perspective
| Open AccessA bioengineering perspective on modelling the intestinal epithelial physiology in vitro
Maria Antfolk and Kim Jensen discuss how to model intestinal epithelial cell function in the dish and how various physiologically important environmental conditions, for example, extracellular matrix, pressure and flow, can be modelled and how this is applicable to clinical work.
- Maria Antfolk
- & Kim B. Jensen
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Article
| Open AccessIntermolecular channels direct crystal orientation in mineralized collagen
Mineralized collagen is the building block of bone but how the collagen directs hydroxyapatite formation remains unclear. Here, the authors demonstrate cylindrical pores in collagen and how the anisotropic growth of hydroxyapatite directs the orientation of crystal growth in mineralized collagen.
- YiFei Xu
- , Fabio Nudelman
- & Nico Sommerdijk
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| Open AccessCharacterization of a pluripotent stem cell-derived matrix with powerful osteoregenerative capabilities
Production of a safe and manufacturable material to mimic anabolic bone for tissue engineering has been hard to achieve to date. Here the authors use a mesenchymal stem cell line generated from induced pluripotent stem cells to produce osteogenic cell-matrix, displaying significant healing properties in mice.
- Eoin P. McNeill
- , Suzanne Zeitouni
- & Carl A. Gregory
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Article
| Open AccessControlled packing and single-droplet resolution of 3D-printed functional synthetic tissues
Precise patterning of lipid-stabilised aqueous droplets is a key challenge in building synthetic tissue designs. Here, the authors show how the interactions between pairs of droplets direct the packing of droplets within 3D-printed networks, enabling the formation of synthetic tissues with high-resolution features.
- Alessandro Alcinesio
- , Oliver J. Meacock
- & Hagan Bayley
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Article
| Open AccessProgrammed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays
To execute higher-order functions, cells self-assemble into spatially coded tissue configurations. Here the authors magnetically assembly giant unilamellar vesicles into three dimensional tissue-mimic structures with collective osmotic stability.
- Qingchuan Li
- , Shubin Li
- & Xiaojun Han
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Article
| Open AccessExtracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture
Organoid cultures have been developed from multiple tissues, opening new possibilities for regenerative medicine. Here the authors demonstrate the derivation of GMP-compliant hydrogels from decellularized porcine small intestine which support formation and growth of human gastric, liver, pancreatic and small intestinal organoids.
- Giovanni Giuseppe Giobbe
- , Claire Crowley
- & Paolo De Coppi
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| Open AccessTear resistance of soft collagenous tissues
Defect tolerance is important in order to ensure the mechanical integrity of synthetic materials and biological tissues. Here the authors demonstrate that toughening mechanisms provide soft collagenous tissues with high defect tolerance, with important implications for biomedical applications.
- Kevin Bircher
- , Manuel Zündel
- & Edoardo Mazza
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Article
| Open AccessPatterned human microvascular grafts enable rapid vascularization and increase perfusion in infarcted rat hearts
Heart grafts need good vascularization to survive. Here, the authors engineer perfusable constructs of human embryonic stem cell-derived endothelial cells seeded in collagen matrix in patterned microchannels that form anastomosed vessels in vitro and have increased coronary vascular perfusion on transplantation in rats.
- Meredith A. Redd
- , Nicole Zeinstra
- & Ying Zheng
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Article
| Open AccessAn integrated multi-layer 3D-fabrication of PDA/RGD coated graphene loaded PCL nanoscaffold for peripheral nerve restoration
Graphene, as a conductive nanomaterial, has potential applications in the restoration of nerve function following physical injury. Here the authors design a graphene scaffold that can improve nerve regeneration.
- Yun Qian
- , Xiaotian Zhao
- & Weien Yuan
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Article
| Open AccessInverse poroelasticity as a fundamental mechanism in biomechanics and mechanobiology
How soft tissues respond to mechanical load is essential to their biological function. Here, the authors discover that – contrary to predictions of poroelasticity – fluid mobility in collagenous tissues induces drastic volume decrease with tensile loading and pronounced chemo-mechanical coupling.
- Alexander E. Ehret
- , Kevin Bircher
- & Edoardo Mazza
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| Open AccessA blueprint for robust crosslinking of mobile species in biogels with weakly adhesive molecular anchors
Biological polymeric matrices often use molecular anchors, such as antibodies, to trap nanoparticulates. Here, the authors find that anchor-matrix bonds that are weak and short-lived confer superior trapping potency, contrary to the prevailing belief that effective molecular anchors should form strong bonds to both the matrix and the nanoparticulates.
- Jay Newby
- , Jennifer L. Schiller
- & Samuel K. Lai
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| Open AccessA 3D magnetic tissue stretcher for remote mechanical control of embryonic stem cell differentiation
The development of embryoid bodies that are responsive to external stimuli is of great interest in tissue engineering. Here, the authors culture embryonic stem cells with magnetic nanoparticles and show that the presence of magnetic fields could affect their aggregation and differentiation.
- Vicard Du
- , Nathalie Luciani
- & Claire Wilhelm
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Article
| Open AccessMolecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides
Collagen denaturation is thought to occur during tissue mechanical damage, but its role in damage initiation is still unclear. Here, the authors use a collagen hybridizing peptide to provide insights into the molecular mechanisms leading to collagen unfolding during tendon mechanical stretch.
- Jared L. Zitnay
- , Yang Li
- & Jeffrey A. Weiss
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Article
| Open AccessTissue engineering of acellular vascular grafts capable of somatic growth in young lambs
Current vessel grafts must be surgically replaced when the recipient outgrows them. Here, Syedain et al.bioengineer a tube of acellular matrix produced from sheep fibroblasts that is capable of cellularizaton and somatic growth when transplanted into growing lambs, eliminating the need for multiple graft surgeries.
- Zeeshan Syedain
- , Jay Reimer
- & Robert T. Tranquillo