Tissue engineering articles within Nature Communications

  • Article
    | Open Access

    Integrating cell-laden hydrogels effectively into the 3D printing process is a challenge in the creation of tissue engineering scaffolds. Here, the authors describe an additive manufacturing technique to combine polymer and cell-containing networks with 3D-printed mechanical supports.

    • Héloïse Ragelle
    • , Mark W. Tibbitt
    •  & Robert Langer
  • Article
    | Open Access

    The generation of functional skeletal muscle tissue from human pluripotent stem cells has not been reported. Here, the authors describe engineering of contractile skeletal muscle bundles in culture, which become vascularized and maintain functionality when transplanted into mice.

    • Lingjun Rao
    • , Ying Qian
    •  & Nenad Bursac
  • Article
    | Open Access

    There is a need for improved in vitro models of host-microbe interactions in the lung. Here, Barkal et al. present a microscale organotypic model of the human bronchiole for studying pulmonary infection, including volatile compound communication between microbial populations and host cells.

    • Layla J. Barkal
    • , Clare L. Procknow
    •  & David J. Beebe
  • Article
    | Open Access

    Dense connective tissues do not easily heal, in part due to a low supply of reparative cells. Here, the authors develop a fibrous scaffold for meniscal repair that sequentially releases collagenase and a growth factor at the injury site, breaking down the extracellular matrix and recruiting endogenous cells.

    • Feini Qu
    • , Julianne L. Holloway
    •  & Robert L. Mauck
  • Article
    | Open Access

    There is a need for humanised grafts to treat patients with intestinal failure. Here, the authors generate intestinal grafts by recellularizing native intestinal matrix with human induced pluripotent stem cell-derived epithelium and human endothelium, and show nutrient absorption after transplantation in rats.

    • Kentaro Kitano
    • , Dana M. Schwartz
    •  & Harald C. Ott
  • Article
    | Open Access

    The fabrication of vascularized 3D tissues requires an understanding of how material properties govern endothelial cell invasion into the surrounding matrix. Here the authors integrate a non-swelling synthetic hydrogel with a microfluidic device to study chemokine gradient-driven angiogenic sprouting and find that matrix degradability modulates the collectivity of cell migration.

    • Britta Trappmann
    • , Brendon M. Baker
    •  & Christopher S. Chen
  • Article
    | Open Access

    The derivation of blood progenitor cells from human pluripotent stem cells is of interest for cell therapy but remains an inefficient process. Here the authors micropattern hPSC-derived haemogenic endothelial (HE) cells into spatially-organized, size-controlled colonies and identify a geometry that achieves increased efficiency in deriving blood cells.

    • Nafees Rahman
    • , Patrick M. Brauer
    •  & Peter W. Zandstra
  • Article
    | Open Access

    Current vessel grafts must be surgically replaced when the recipient outgrows them. Here, Syedain et al.bioengineer a tube of acellular matrix produced from sheep fibroblasts that is capable of cellularizaton and somatic growth when transplanted into growing lambs, eliminating the need for multiple graft surgeries.

    • Zeeshan Syedain
    • , Jay Reimer
    •  & Robert T. Tranquillo
  • Article
    | Open Access

    Generating organized kidney tissues from human pluripotent stem cell is a major challenge. Here, Freedman et al. describe a differentiation system forming spheroids and tubular structures, characteristic of these kidney structures, and using CRISPR/Cas9, delete PKD1/2, to model polycystic kidney disease.

    • Benjamin S. Freedman
    • , Craig R. Brooks
    •  & Joseph V. Bonventre
  • Article
    | Open Access

    Organogenesis is orchestrated by biochemical and biophysical stimuli. Here, Ma et al. generate a micro-patterned surface that provides mechanical cues which, when combined with biochemical signals, drive human pluripotent stem cells’ differentiation into beating cardiac microchambers resembling primitive hearts.

    • Zhen Ma
    • , Jason Wang
    •  & Kevin E. Healy
  • Article
    | Open Access

    Avoiding central cell necrosis at the centre of large engineered tissue constructs is an important issue forin vitrotissue engineering. Here, the authors demonstrate that this problem may be overcome by oxygenating human mesenchymal stem cells with artificial membrane-binding proteins.

    • James P. K. Armstrong
    • , Rameen Shakur
    •  & Anthony P. Hollander
  • Article |

    Hydrogels are commonly used materials for tissue engineering, but they can lack the structural properties required for load-bearing and mechanical applications. Here, the authors prepare a polycaprolactone scaffold using melt-electrospinning to reinforce a gelatin methacrylamide hydrogel.

    • Jetze Visser
    • , Ferry P.W. Melchels
    •  & Jos Malda
  • Article
    | Open Access

    It is known that skin has a large tear resistance, but little is known of the mechanism behind this. Here, the authors carry out a structural analysis of rabbit skin to show how the deformation of collagen fibrils in the skin results in a strong resistance to tear propagation.

    • Wen Yang
    • , Vincent R. Sherman
    •  & Marc A. Meyers
  • Article
    | Open Access

    Patients with oesophageal diseases may require surgical removal and replacement of the oesophagus. Here the authors seed mesenchymal stromal cells on a decellularized rat oesophagus and show that this bioengineered tissue construct restores swallowing function after transplantation into rats.

    • Sebastian Sjöqvist
    • , Philipp Jungebluth
    •  & Paolo Macchiarini
  • Article |

    Traditional methods for forming hydrogel particles are limited by geometry and lack of addressability after synthesis. Here the authors use digital microfluidics to form individually addressable gels with customisable shapes and compositions.

    • Irwin A. Eydelnant
    • , Bingyu Betty Li
    •  & Aaron R. Wheeler
  • Article |

    The hair follicle bulge contains epithelial stem cells that contribute to follicle formation during each hair cycle. Here the authors differentiate human induced pluripotent stem cells into folliculogenic epithelial stem cells, which can produce all hair follicle lineages including a stem cell population.

    • Ruifeng Yang
    • , Ying Zheng
    •  & Xiaowei Xu
  • Article |

    Tissue engineering relies on the vascular compatibility of the synthesised constructs with target tissues. Here, the authors fabricate a prevascularised tissue construct of cell-laden hydrogel fibres as a framework that allows the formation of vascularised adipose and hepatic tissues.

    • Meng Fatt Leong
    • , Jerry K. C. Toh
    •  & Jackie Y. Ying
  • Article |

    Artificially engineered tissues may be useful for regenerative therapies but their fabrication tends to be complicated. Stevens et al. present a technique for the precise organization of microstructurally complex tissues that works with a variety of cell types and does not require sophisticated equipment.

    • K. R. Stevens
    • , M. D. Ungrin
    •  & S. N. Bhatia
  • Article
    | Open Access

    Artificially engineered tissues may have many therapeutic applications but complex tissues are hard to create in vitro. Here, Okano and colleagues report the production of functional cardiac tissue sheets with perfusable blood vessels, which increase the thickness and survival of transplanted tissue.

    • Hidekazu Sekine
    • , Tatsuya Shimizu
    •  & Teruo Okano