Replisome articles within Nature Communications

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  • Article
    | Open Access

    Here the authors present the structure of Replication Protein A (RPA) in Archaea. The RPA structure from P. abyssi has been determined in presence and absence of DNA, providing insights into the evolution of this replication factor in eukaryotes

    • Clément Madru
    • , Markel Martínez-Carranza
    •  & Ludovic Sauguet

  • Article
    | Open Access

    Profiling of human DNA polymerase Polε and Polα demonstrates their roles in leading and lagging strand DNA synthesis, and their independent measures allowed accurate predictions of replication dynamics and effects of transcription.

    • Eri Koyanagi
    • , Yoko Kakimoto
    •  & Yasukazu Daigaku

  • Article
    | Open Access

    DNA polymerase epsilon has a critical role in DNA replication initiation. Here, the authors show that in human cancer cells POLE is dispensable for the replicative helicase assembly but not for replication initiation, which requires the non-catalytic domain of POLE1.

    • Sameera Vipat
    • , Dipika Gupta
    •  & Tatiana N. Moiseeva

  • Article
    | Open Access

    This study describes how p97Ufd1-Npl4 and the UBA-UBX protein Ubxn7 disassemble vertebrate replisomes during replication termination, and it provides novel insights into how p97 complexes assemble with UBA-UBX proteins on ubiquitylated substrates

    • Olga V. Kochenova
    • , Sirisha Mukkavalli
    •  & Johannes C. Walter

  • Article
    | Open Access

    DNA replication of repetitive sequences was recreated in a test tube using purified components. DNA alone was sufficient to induce stalling. Both stalling and recovery were dictated by the capacity of DNA to fold into unusual secondary structures.

    • Corella S. Casas-Delucchi
    • , Manuel Daza-Martin
    •  & Gideon Coster

  • Article
    | Open Access

    How nucleosome assembly of parental histones is regulated following DNA replication is still an open question. Here the authors show that unlike deposition of new histones H3.1 and H3.3 that utilizes different histone chaperones, parental H3.1 and H3.3 are both stably inherited during mitotic cell division in mouse embryonic stem cells, and this involves histone chaperones Mcm2, Pole3 and Pole4.

    • Xiaowei Xu
    • , Shoufu Duan
    •  & Zhiguo Zhang

  • Article
    | Open Access

    In the genome, repetitive guanine-rich sequences have the potential to spontaneously fold into non-canonical DNA secondary structures known as G-quadruplex (G4). Using novel single-molecule imaging approaches, the authors reveal that G4 formation within active replication forks locally perturb replisome dynamics and damage response signaling, which require RPA and FANCJ for regulation.

    • Wei Ting C. Lee
    • , Yandong Yin
    •  & Eli Rothenberg

  • Article
    | Open Access

    The fork protection complex (FPC), including the proteins TIMELESS and TIPIN, stabilizes the replisome to ensure unperturbed fork progression during DNA replication. Here the authors reveal that that SDE2, a PCNA-associated protein, plays an important role in maintaining active replication and protecting stalled forks by regulating the replication fork protection complex (FPC).

    • Julie Rageul
    • , Jennifer J. Park
    •  & Hyungjin Kim

  • Article
    | Open Access

    The origin recognition complex (ORC) is essential for loading the Mcm2–7 replicative helicase onto DNA during DNA replication initiation. Here, the authors describe several cryo-electron microscopy structures of Drosophila ORC bound to DNA and its cofactor Cdc6 and also report an in vitro reconstitution system for Drosophila Mcm2–7 loading, revealing unexpected features of ORC’s DNA binding and remodeling mechanism during Mcm2–7 loading.

    • Jan Marten Schmidt
    •  & Franziska Bleichert

  • Article
    | Open Access

    Whereas the toxic effects of ethanol are well-documented, the underlying mechanism is obscure. This study uses the eukaryotic model S. cerevisiae to reveal how exposure to sublethal ethanol concentrations causes DNA replication stress and an increased mutation rate.

    • Karin Voordeckers
    • , Camilla Colding
    •  & Kevin J. Verstrepen

  • Article
    | Open Access

    Replicative DNA polymerases (DNAPs) have evolved the ability to copy the genome with high processivity and fidelity. Here, the authors present a cryo-EM structure of the DNA-bound PolD–PCNA complex from Pyrococcus abyssi to reveal the molecular basis for the interaction and cooperativity between a replicative DNAP and PCNA.

    • Clément Madru
    • , Ghislaine Henneke
    •  & Ludovic Sauguet

  • Article
    | Open Access

    Eukaryotes and archaea use a heximeric ring-shaped MCM helicase to unwind the DNA template during replication. Here the authors present a crystal structure of the MCM complex from archaeon S. solfataricus bound to single-stranded DNA, and to a combination of ADP, and ATP-mimic, ADP-BeF3.

    • Martin Meagher
    • , Leslie B. Epling
    •  & Eric J. Enemark

  • Article
    | Open Access

    Eukaryotic origin firing depends on assembly of the Cdc45-MCM-GINS (CMG) helicase, which requires the leading-strand polymerase Pol ɛ. Here the authors present a structural analysis of a CMG Pol ɛ on a DNA fork, providing insight on the steps leading productive helicase engagement to the DNA junction.

    • Panchali Goswami
    • , Ferdos Abid Ali
    •  & Alessandro Costa

  • Article
    | Open Access

    AND-1, the vertebrate orthologue of Ctf4, is a critical player during DNA replication and for maintenance of genome integrity. Here the authors use a conditional AND-1 depletion system in avian DT40 cells to reveal the consequences of the lack of AND-1 on cell proliferation and DNA replication.

    • Takuya Abe
    • , Ryotaro Kawasumi
    •  & Dana Branzei

  • Article
    | Open Access

    During DNA replication, replicative helicases play an essential role for DNA unwinding to occur. Here the authors find that bacteriophage T7 helicase is also involved in replication re-initiation by interacting with a non-replicating DNAP and increasing unwinding rate.

    • Bo Sun
    • , Anupam Singh
    •  & Michelle D. Wang

  • Article
    | Open Access

    The maintenance of chromatin integrity during replication is critical for cell viability. Here the authors study how dividing cells respond to alterations in chromatin structure and find that these elicit a range of responses in the dynamics of DNA replication and consequences on replicative stress.

    • Ricardo Almeida
    • , José Miguel Fernández-Justel
    •  & María Gómez

  • Article
    | Open Access

    DNA polymerases δ and ε (Pols δ and ε) are thought to be responsible for lagging and leading strand synthesis, respectively. Here the authors present evidence that Pol δ contributes to the initiation of leading strand replication in budding yeast by synthesizing DNA of both strands at replication origins.

    • Marta A. Garbacz
    • , Scott A. Lujan
    •  & Thomas A. Kunkel

  • Article
    | Open Access

    The loading and activation of the Mcm2-7 replicative helicase couples cell cycle progression to DNA replication. Here the authors use X-ray crystallography and single-particle electron microscopy to demonstrate how Ctd1 functions to promote MCM loading onto DNA.

    • Jordi Frigola
    • , Jun He
    •  & John F. X. Diffley

  • Article
    | Open Access

    PrimPol is a multifunctional replicative enzyme that can bypass DNA damage, as well as reprime replication restart. Here, the authors have elucidated how PrimPol is recruited to stalled replication forks via specific interactions with RPA, which stimulates its primase activity.

    • Thomas A. Guilliam
    • , Nigel C. Brissett
    •  & Aidan J. Doherty

  • Article
    | Open Access

    DNA sliding clamps are ring-shaped proteins that encircle DNA and harbour polymerases and other factors that promote processive DNA replication. Here the authors use X-ray crystallography, NMR and MD simulations to propose a model for a PCNA sliding mechanism that relies on short-lived polar interactions.

    • Matteo De March
    • , Nekane Merino
    •  & Alfredo De Biasio

  • Article
    | Open Access

    Genomic instability can result from stalled or collapsed replication fork at sites of unrepaired DNA lesions. Here the authors uncover a new lesion bypass pathway for the T7 replisome, where leading strand template lesions can be overcome through interaction between the replisome's helicase and polymerase components.

    • Bo Sun
    • , Manjula Pandey
    •  & Michelle D. Wang

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