Parasite development

  • Article | | open

    Transcriptomic analysis often doesn’t differentiate between newly synthesized and stabilized mRNAs. Using rapid 4-thiouracil incorporation, Painter et al. here define genome-wide active transcription throughout Plasmodium blood-stage developmental stages and identify associated regulatory DNA sequence motifs.

    • Heather J. Painter
    • , Neo Christopher Chung
    • , Aswathy Sebastian
    • , Istvan Albert
    • , John D. Storey
    •  & Manuel Llinás
  • Article | | open

    Malaria transmission is effected by intra-erythrocytic parasites that commit to sexual development and form gametocytes. Here, the authors show that early reticulocytes in the major sites of haematopoiesis establish a cryptic asexual cycle; this cycle is characterised by early preferential commitment to gametocytogenesis, which initiates malaria transmission and drug resistance.

    • Rebecca S. Lee
    • , Andrew P. Waters
    •  & James M. Brewer
  • Article | | open

    For reproduction, the human parasite Schistosoma japonicum relies on a complex and incompletely understood interplay between female and male schistosomes. Here the authors sequence the transcriptome of female and male schistosomes across eight time points during sexual development.

    • Jipeng Wang
    • , Ying Yu
    • , Haimo Shen
    • , Tao Qing
    • , Yuanting Zheng
    • , Qing Li
    • , Xiaojin Mo
    • , Shuqi Wang
    • , Nana Li
    • , Riyi Chai
    • , Bin Xu
    • , Mu Liu
    • , Paul J Brindley
    • , Donald P McManus
    • , Zheng Feng
    • , Leming Shi
    •  & Wei Hu
  • Article | | open

    Oocyst rupture and release of malaria sporozoites is needed for transmission of parasites from vector to humans. Here the authors identify two proteins, which they name ORP1 and ORP2, that form heterodimers and are required for oocyst rupture.

    • Chiara Currà
    • , Renate Gessmann
    • , Tomasino Pace
    • , Leonardo Picci
    • , Giulia Peruzzi
    • , Vassiliki Varamogianni-Mamatsi
    • , Lefteris Spanos
    • , Célia R. S. Garcia
    • , Roberta Spaccapelo
    • , Marta Ponzi
    •  & Inga Siden-Kiamos
  • Article | | open

    Giardia lamblia is a human protozoan parasite with two diploid nuclei, which makes complete knock-out of a gene of interest challenging. Here the authors use a Cre/loxP-based approach to knock-out cyst-wall protein 1 (cwp1) and show that CWP1 is essential for cyst-wall biosynthesis.

    • Jacqueline A. Ebneter
    • , Sally D. Heusser
    • , Elisabeth M. Schraner
    • , Adrian B. Hehl
    •  & Carmen Faso
  • Article | | open

    The functions of many putative membrane transport proteins of malaria parasites are unknown. Here, Kenthirapalan et al. use mutant strains carrying targeted gene deletions to study the functions of 35 such proteins during the life cycle of Plasmodium berghei in mosquito and mouse hosts.

    • Sanketha Kenthirapalan
    • , Andrew P. Waters
    • , Kai Matuschewski
    •  & Taco W. A. Kooij
  • Article | | open

    The chronic disease schistosomiasis is caused by the blood fluke Schistosoma mansoni. By studying DNA modifications throughout the lifecycle of the pathogen, the authors identify DNA methylation as a factor in egg development and suggest that the epigenetic machinery responsible may be a therapeutic target.

    • Kathrin K. Geyer
    • , Carlos M. Rodríguez López
    • , Iain W. Chalmers
    • , Sabrina E. Munshi
    • , Martha Truscott
    • , James Heald
    • , Mike J. Wilkinson
    •  & Karl F. Hoffmann