Optical imaging

Optical imaging includes a variety of imaging techniques that rely on illumination light in the ultraviolet, visible and infrared regions of the electromagnetic spectrum. The term typically excludes classical microscopy techniques in favour of larger scale imaging methods that rely on the detection of ballistic or diffusive photons, or the photoacoustic effect.

Latest Research and Reviews

  • Research |

    Two optical signatures of amyloid fibres—luminescence in the blue and a near-infrared signal, which can be observed in in vitro and in vivo tissues—are reported. The findings allow for staining-free characterization of amyloid deposits in human samples and could open the door to innovative diagnostic strategies for neurodegenerative diseases.

    • Jonathan Pansieri
    • , Véronique Josserand
    • , Sun-Jae Lee
    • , Anaëlle Rongier
    • , Daniel Imbert
    • , Marcelle Moulin Sallanon
    • , Enikö Kövari
    • , Thomas G. Dane
    • , Charlotte Vendrely
    • , Odette Chaix-Pluchery
    • , Mélanie Guidetti
    • , Julien Vollaire
    • , Arnold Fertin
    • , Yves Usson
    • , Patrice Rannou
    • , Jean-Luc Coll
    • , Christel Marquette
    •  & Vincent Forge
  • Research |

    Photoacoustic detection highly localized with a pulsed ultraviolet laser based on the Grüneisen relaxation effect allows water-background suppressed mid-infrared (MIR) imaging of lipids and proteins at ultraviolet resolution, at least an order of magnitude finer than the MIR diffraction limits.

    • Junhui Shi
    • , Terence T. W. Wong
    • , Yun He
    • , Lei Li
    • , Ruiying Zhang
    • , Christopher S. Yung
    • , Jeeseong Hwang
    • , Konstantin Maslov
    •  & Lihong V. Wang
  • Research |

    Light-sheet microscopy in the NIR-II window enables rapid volumetric imaging of tissues at impressive depths in vivo without invasive preparations owing to the reduced light scattering and tissue autofluorescence at these wavelengths.

    • Feifei Wang
    • , Hao Wan
    • , Zhuoran Ma
    • , Yeteng Zhong
    • , Qinchao Sun
    • , Ye Tian
    • , Liangqiong Qu
    • , Haotian Du
    • , Mingxi Zhang
    • , Lulin Li
    • , Huilong Ma
    • , Jian Luo
    • , Yongye Liang
    • , Wen Jung Li
    • , Guosong Hong
    • , Lianqing Liu
    •  & Hongjie Dai
  • Protocols |

    This protocol for clearing and high-resolution 3D imaging of entire organoids expressing fluorescence reporters or following immunolabeling enables confocal, super-resolution confocal, multiphoton and light-sheet microscopy to be performed.

    • Johanna F. Dekkers
    • , Maria Alieva
    • , Lianne M. Wellens
    • , Hendrikus C. R. Ariese
    • , Paul R. Jamieson
    • , Annelotte M. Vonk
    • , Gimano D. Amatngalim
    • , Huili Hu
    • , Koen C. Oost
    • , Hugo J. G. Snippert
    • , Jeffrey M. Beekman
    • , Ellen J. Wehrens
    • , Jane E. Visvader
    • , Hans Clevers
    •  & Anne C. Rios

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