Multiphoton microscopy

Multiphoton microscopy is a general term for a variety of fluorescence and non-fluorescence microscopy techniques that rely on multiple photons interacting with either individual molecules or optical heterogeneities in a sample. These techniques generally rely on pulsed high-intensity lasers and have better sample penetration than single-photon techniques.

Latest Research and Reviews

News and Comment

  • News & Views |

    Producing reliable atomic- or close-to-atomic-resolution structures of RNA-only molecules has been a formidable task. Ribosolve can solve sub-nanometer-resolution cryo-EM structures of unbound RNA molecules with unprecedented accuracy and speed.

    • Jane S. Richardson
    Nature Methods 17, 663-664
  • News & Views |

    Caged neurotransmitters are widely used to study neurobiological processes such as synaptic transmission and plasticity. However, uncaging has been primarily restricted to in vitro and ex vivo experimental systems. Using caged neurotransmitters in vivo has posed a huge hurdle because photoactivatable cages bind to GABA-A receptors, acting as competitive antagonists towards GABA. This reduced inhibition leads to epileptiform-like activity, which can cause problems for circuit level studies in vivo. To circumvent this off-target effect, a recent publication introduces a new caged glutamate: G5-MNI-glutamate. Using a novel technique called ‘cloaking,’ GABA-A receptor antagonism is abolished, opening up new possibilities for future in vivo studies with caged neurotransmitters.

    • Roberto Ogelman
    • , In-Wook Hwang
    •  & Won Chan Oh
    Lab Animal 49, 177-179
  • News |

    How joy and wide-ranging curiosity leads to neurobiology tools for new types of questions.

    • Vivien Marx
    Nature Methods 17, 245