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| Open AccessA novel bioassay for quantification of surface Cannabinoid receptor 1 expression
- Ismael Rodríguez-Rodríguez
- , Joanna Kalafut
- & Adolfo Rivero-Müller
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| Open AccessStructure-based design and discovery of novel anti-tissue factor antibodies with cooperative double-point mutations, using interaction analysis
- Shuntaro Chiba
- , Aki Tanabe
- & Masateru Ohta
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| Open AccessCovalent protein display on Hepatitis B core-like particles in plants through the in vivo use of the SpyTag/SpyCatcher system
- Hadrien Peyret
- , Daniel Ponndorf
- & George P. Lomonossoff
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| Open AccessAncestral sequence reconstruction produces thermally stable enzymes with mesophilic enzyme-like catalytic properties
- Ryutaro Furukawa
- , Wakako Toma
- & Satoshi Akanuma
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| Open AccessIMPROvER: the Integral Membrane Protein Stability Selector
- Steven P. D. Harborne
- , Jannik Strauss
- & Adrian Goldman
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| Open AccessEngineered viral DNA polymerase with enhanced DNA amplification capacity: a proof-of-concept of isothermal amplification of damaged DNA
- Carlos D. Ordóñez
- , Ana Lechuga
- & Modesto Redrejo-Rodríguez
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| Open AccessNearest-neighbor amino acids of specificity-determining residues influence the activity of engineered Cre-type recombinases
- Anjali Soni
- , Martina Augsburg
- & M. Teresa Pisabarro
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| Open AccessOptimizing bacteriophage engineering through an accelerated evolution platform
- Andrew H. Favor
- , Carlos D. Llanos
- & Jorge A. Bardales
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| Open AccessRapid repair of human disease-specific single-nucleotide variants by One-SHOT genome editing
- Yuji Yokouchi
- , Shinichi Suzuki
- & Takumi Era
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| Open AccessPlasmid-based complementation of large deletions in Phaeodactylum tricornutum biosynthetic genes generated by Cas9 editing
- Samuel S. Slattery
- , Helen Wang
- & David R. Edgell
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| Open AccessCRISPR/Cas12a mediated knock-in of the Polled Celtic variant to produce a polled genotype in dairy cattle
- Felix Schuster
- , Patrick Aldag
- & Björn Petersen
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| Open AccessEngineering a disulfide-gated switch in streptavidin enables reversible binding without sacrificing binding affinity
- Jesse M. Marangoni
- , Sau-Ching Wu
- & Kenneth K. S. Ng
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| Open AccessRecombinant expression, purification and PEGylation of Paneth cell peptide (cryptdin-2) with value added attributes against Staphylococcus aureus
- Navneet Kaur
- , Rahul Dilawari
- & Praveen Rishi
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| Open AccessDiB-splits: nature-guided design of a novel fluorescent labeling split system
- Nina G. Bozhanova
- , Alexey S. Gavrikov
- & Jens Meiler
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| Open AccessRational identification and characterisation of peptide ligands for targeting polysialic acid
- Divya G. Shastry
- , Flaviyan Jerome Irudayanathan
- & Pankaj Karande
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| Open AccessMolecular insights and cell cycle assessment upon exposure to Chaga (Inonotus obliquus) mushroom polysaccharides in zebrafish (Danio rerio)
- Jehane Ibrahim Eid
- & Biswadeep Das
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| Open AccessOverexpression of V-type H+ pyrophosphatase gene EdVP1 from Elymus dahuricus increases yield and potassium uptake of transgenic wheat under low potassium conditions
- Yongbin Zhou
- , Yan Li
- & Ming Chen
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| Open AccessBuild-up functionalization of anti-EGFR × anti-CD3 bispecific diabodies by integrating high-affinity mutants and functional molecular formats
- Ryutaro Asano
- , Katsuhiro Hosokawa
- & Izumi Kumagai
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| Open AccessEpistatic Effect of Regulators to the Adaptive Growth of Escherichia coli
- Yukari Miyake
- & Kaneyoshi Yamamoto
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Article
| Open AccessRational Design of Photo-Electrochemical Hybrid Devices Based on Graphene and Chlamydomonas reinhardtii Light-Harvesting Proteins
- Martha I. Ortiz-Torres
- , Miguel Fernández-Niño
- & Andrés Fernando González Barrios
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| Open AccessEvolved Fusarium oxysporum laccase expressed in Saccharomyces cerevisiae
- Natalia Kwiatos
- , Marzena Jędrzejczak-Krzepkowska
- & Stanisław Bielecki
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| Open AccessReplacing the eleven native tryptophans by directed evolution produces an active P-glycoprotein with site-specific, non-conservative substitutions
- Douglas J. Swartz
- , Anukriti Singh
- & Ina L. Urbatsch
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| Open AccessRecombinant HIV-1 vaccine candidates based on replication-defective flavivirus vector
- M. Giel-Moloney
- , M. Esteban
- & K. V. Pugachev
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| Open AccessDevelopment of hypoallergenic variants of the major horse allergen Equ c 1 for immunotherapy by rational structure based engineering
- Jaana Haka
- , Merja H. Niemi
- & Juha Rouvinen
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| Open AccessA qPCR method for genome editing efficiency determination and single-cell clone screening in human cells
- Bo Li
- , Naixia Ren
- & Qilai Huang
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| Open AccessAn improved overlap extension PCR for simultaneous multiple sites large fragments insertion, deletion and substitution
- Wei Guo
- , Binhua Xie
- & Zhong-Min Dai
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| Open AccessmRhubarb: Engineering of monomeric, red-shifted, and brighter variants of iRFP using structure-guided multi-site mutagenesis
- Oliver C. Rogers
- , Dorothy M. Johnson
- & Elad Firnberg
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| Open AccessDissecting the evolvability landscape of the CalB active site toward aromatic substrates
- Yossef López de los Santos
- , Ying Lian Chew-Fajardo
- & Nicolas Doucet
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| Open AccessStructural analysis of biological targets by host:guest crystal lattice engineering
- Patrick Ernst
- , Andreas Plückthun
- & Peer R. E. Mittl
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| Open AccessStructural and In Vitro Functional Analyses of Novel Plant-Produced Anti-Human PD1 Antibody
- Kaewta Rattanapisit
- , Tanapati Phakham
- & Waranyoo Phoolcharoen
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| Open AccessFusion proteins with chromogenic and keratin binding modules
- Ana Tinoco
- , Egipto Antunes
- & Artur Ribeiro
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| Open AccessRegulation of gene expression by altered promoter methylation using a CRISPR/Cas9-mediated epigenetic editing system
- Jeong Gu Kang
- , Jin Suk Park
- & Yong-Sam Kim
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Article
| Open AccessEngineering a Polyspecific Pyrrolysyl-tRNA Synthetase by a High Throughput FACS Screen
- Adrian Hohl
- , Ram Karan
- & Jörg Eppinger
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| Open AccessOptimization of a reduced enzymatic reaction cascade for the production of L-alanine
- Tobias J. Gmelch
- , Josef M. Sperl
- & Volker Sieber
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| Open AccessGolden Mutagenesis: An efficient multi-site-saturation mutagenesis approach by Golden Gate cloning with automated primer design
- Pascal Püllmann
- , Chris Ulpinnis
- & Martin J. Weissenborn
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| Open AccessDevelopment of latent Interferon alpha 2b as a safe therapeutic for treatment of Hepatitis C virus infection
- Iram Gull
- , Muhammad Shahbaz Aslam
- & Muhammad Amin Athar
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| Open AccessA suite of kinetically superior AEP ligases can cyclise an intrinsically disordered protein
- Karen S. Harris
- , Rosemary F. Guarino
- & Marilyn A. Anderson
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| Open AccessPhosphite binding by the HtxB periplasmic binding protein depends on the protonation state of the ligand
- Nathan B. P. Adams
- , Angus J. Robertson
- & Claudine Bisson
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| Open AccessExosomes Engineered to Express a Cardiomyocyte Binding Peptide Demonstrate Improved Cardiac Retention in Vivo
- Kyle I. Mentkowski
- & Jennifer K. Lang
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| Open AccessUse of syngeneic cells expressing membrane-bound GM-CSF as an adjuvant to induce antibodies against native multi-pass transmembrane protein
- Chien-Chiao Huang
- , Kai-Wen Cheng
- & Tian-Lu Cheng
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| Open AccessSingle-Molecule 3D Images of “Hole-Hole” IgG1 Homodimers by Individual-Particle Electron Tomography
- Dongsheng Lei
- , Jianfang Liu
- & Gang Ren
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| Open AccessExploring the sequence variability of polymerization-involved residues in the production of levan- and inulin-type fructooligosaccharides with a levansucrase
- Christian Possiel
- , Maria Elena Ortiz-Soto
- & Jürgen Seibel
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| Open AccessDevelopment of a ribonuclease containing a G4-specific binding motif for programmable RNA cleavage
- Dung Thanh Dang
- & Anh Tuân Phan
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| Open AccessIn vitro evolution of enhanced RNA replicons for immunotherapy
- Yingzhong Li
- , Brian Teague
- & Ron Weiss
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| Open AccessThe Structural Dynamics of Engineered β-Lactamases Vary Broadly on Three Timescales yet Sustain Native Function
- Sophie M. C. Gobeil
- , Maximillian C. C. J. C. Ebert
- & Joelle N. Pelletier
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| Open AccessStructure-based Design of JOC-x, a Conjugatable Tumor Tight Junction Opener to Enhance Cancer Therapy
- Ragan Pitner
- , Jiho Kim
- & Sean A. Gray
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| Open AccessBioproduction of pure, kilobase-scale single-stranded DNA
- Tyson R. Shepherd
- , Rebecca R. Du
- & Mark Bathe
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| Open AccessIntrogression of a synthetic sex ratio distortion system from Anopheles gambiae into Anopheles arabiensis
- Federica Bernardini
- , Antonios Kriezis
- & Andrea Crisanti
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| Open AccessPosition 123 of halohydrin dehalogenase HheG plays an important role in stability, activity, and enantioselectivity
- Jennifer Solarczek
- , Thomas Klünemann
- & Anett Schallmey