Microbiology

  • Article | | open

    PurR acts as transcriptional repressor of purine biosynthesis genes in some bacterial species. Here, the authors show that purR mutations can arise in Staphylococcus aureus upon exposure to stress, leading to upregulation of fibronectin-binding proteins and increased virulence.

    • Mariya I. Goncheva
    • , Ronald S. Flannagan
    • , Brigid E. Sterling
    • , Holly A. Laakso
    • , Nancy C. Friedrich
    • , Julienne C. Kaiser
    • , David W. Watson
    • , Christy H. Wilson
    • , Jessica R. Sheldon
    • , Martin J. McGavin
    • , Patti K. Kiser
    •  & David E. Heinrichs
  • Article | | open

    SIRPa is most commonly known as a phagocytosis inhibitory receptor expressed by myeloid cells. Here the authors show SIRPa is expressed on a subset of CD8+ T cells with higher proliferative and effector activity during the chronic phase of the immune response to viral infection.

    • Lara M. Myers
    • , Michal Caspi Tal
    • , Laughing Bear Torrez Dulgeroff
    • , Aaron B. Carmody
    • , Ronald J. Messer
    • , Gunsagar Gulati
    • , Ying Ying Yiu
    • , Matthew M. Staron
    • , Cesar Lopez Angel
    • , Rahul Sinha
    • , Maxim Markovic
    • , Edward A. Pham
    • , Benjamin Fram
    • , Aijaz Ahmed
    • , Aaron M. Newman
    • , Jeffrey S. Glenn
    • , Mark M. Davis
    • , Susan M. Kaech
    • , Irving L. Weissman
    •  & Kim J. Hasenkrug
  • Article | | open

    Simple, fast, and accurate detection of food freshness has great significance to food safety and business. Here, the authors develop cellulose-based ratiometric fluorescent materials with superior amine-response, which can be used for visual monitoring the freshness of shrimp and crab.

    • Ruonan Jia
    • , Weiguo Tian
    • , Haotian Bai
    • , Jinming Zhang
    • , Shu Wang
    •  & Jun Zhang
  • Article | | open

    Infection of a cell with influenza B virus (IBV) often results in cell death and the role of surviving cells in pathogenesis is unclear. Here, Dumm et al. generate a recombinant IBV that activates a host-cell reporter to permanently label infected cells, and show that surviving cells are important to preserve epithelial barrier function.

    • Rebekah E. Dumm
    • , Jessica K. Fiege
    • , Barbara M. Waring
    • , Chay T. Kuo
    • , Ryan A. Langlois
    •  & Nicholas S. Heaton
  • Article | | open

    Pseudomonas aeruginosa releases redox-active metabolites called phenazines. Here, the authors use metabolic imaging by stimulated Raman scattering microscopy to show that phenazines antagonize the effects of antibiotics on P. aeruginosa biofilms by modulating bacterial metabolism.

    • Konstanze T. Schiessl
    • , Fanghao Hu
    • , Jeanyoung Jo
    • , Sakila Z. Nazia
    • , Bryan Wang
    • , Alexa Price-Whelan
    • , Wei Min
    •  & Lars E. P. Dietrich
  • Article | | open

    The fusion peptide (FP) of HIV envelope (Env) is critical in the cell entry process. Here, Kumar et al. present crystal structures of B41 SOSIP.664 Env trimer and show the dynamic nature of the FP and proximal region, which likely relates to conformational rearrangements required for membrane fusion.

    • Sonu Kumar
    • , Anita Sarkar
    • , Pavel Pugach
    • , Rogier W. Sanders
    • , John P. Moore
    • , Andrew B. Ward
    •  & Ian A. Wilson
  • Article | | open

    Viruses can encode genes that regulate the host's translational machinery to their advantage. Here, the authors show that viruses encode ribosomal proteins that can be incorporated into the host’s ribosome and may affect translation.

    • Carolina M. Mizuno
    • , Charlotte Guyomar
    • , Simon Roux
    • , Régis Lavigne
    • , Francisco Rodriguez-Valera
    • , Matthew B. Sullivan
    • , Reynald Gillet
    • , Patrick Forterre
    •  & Mart Krupovic
  • Article | | open

    Phospholipase C β (PLCβ) exhibits immuno-modulatory functions but its role in antiviral innate responses is unclear. Here, the authors provide evidence that PLCβ2 down regulates enterovirus-induced pro-inflammatory responses via inhibition of TAK1 activation, and suggest PLC as a potential therapeutic target.

    • Lin Wang
    • , Yilong Zhou
    • , Zijuan Chen
    • , Lei Sun
    • , Juehui Wu
    • , Haohao Li
    • , Feng Liu
    • , Fei Wang
    • , Chunfu Yang
    • , Juhao Yang
    • , Qibin Leng
    • , Qingli Zhang
    • , Ajing Xu
    • , Lisong Shen
    • , Jinqiao Sun
    • , Dianqing Wu
    • , Caiyun Fang
    • , Haojie Lu
    • , Dapeng Yan
    •  & Baoxue Ge
  • Article | | open

    It is unclear whether CO2-stimulation of photosynthesis can propagate through slower ecosystem processes and lead to long-term increases in terrestrial carbon. Here the authors show that CO2-stimulation of photosynthesis leads to a 30% increase in forest regrowth over a decade of CO2 enrichment.

    • Anthony P. Walker
    • , Martin G. De Kauwe
    • , Belinda E. Medlyn
    • , Sönke Zaehle
    • , Colleen M. Iversen
    • , Shinichi Asao
    • , Bertrand Guenet
    • , Anna Harper
    • , Thomas Hickler
    • , Bruce A. Hungate
    • , Atul K. Jain
    • , Yiqi Luo
    • , Xingjie Lu
    • , Meng Lu
    • , Kristina Luus
    • , J. Patrick Megonigal
    • , Ram Oren
    • , Edmund Ryan
    • , Shijie Shu
    • , Alan Talhelm
    • , Ying-Ping Wang
    • , Jeffrey M. Warren
    • , Christian Werner
    • , Jianyang Xia
    • , Bai Yang
    • , Donald R. Zak
    •  & Richard J. Norby
  • Article | | open

    Here, Depledge et al. use nanopore arrays for direct RNA sequencing to profile the HSV-1 transcriptome in productively infected cells. Sequencing of individual RNAs reveals a highly complex viral transcriptome including mRNAs encoding new viral fusion proteins derived by read-through transcription.

    • Daniel P. Depledge
    • , Kalanghad Puthankalam Srinivas
    • , Tomohiko Sadaoka
    • , Devin Bready
    • , Yasuko Mori
    • , Dimitris G. Placantonakis
    • , Ian Mohr
    •  & Angus C. Wilson
  • Article | | open

    Adenovirus based (AdV) vectors are promising platforms for therapeutics and vaccines, but receptor usage of serotypes in clinical development remains unclear. Here, based on crystal structures and modeling, Baker et al. show that HAdV-D26/48 fiber knob protein interacts weakly with CAR but not with CD46 or DSG2.

    • Alexander T. Baker
    • , Alexander Greenshields-Watson
    • , Lynda Coughlan
    • , James A. Davies
    • , Hanni Uusi-Kerttula
    • , David K. Cole
    • , Pierre J. Rizkallah
    •  & Alan L. Parker
  • Article | | open

    Historical and future trends in net primary productivity (NPP) and its sensitivity to global change are largely unknown because of the lack of long-term, high-resolution data. Here the authors show that tree-ring isotopes can be used for inferring interannual variability and long-term changes in NPP.

    • Mathieu Levesque
    • , Laia Andreu-Hayles
    • , William Kolby Smith
    • , A. Park Williams
    • , Martina L. Hobi
    • , Brady W. Allred
    •  & Neil Pederson
  • Article | | open

    Few approaches for targeted manipulation of the epigenome are available in plants. Here, the authors adapt the dCas9-SunTag system to engineer targeted gene activation and site-specific manipulation of DNA methylation in Arabidopsis.

    • Ashot Papikian
    • , Wanlu Liu
    • , Javier Gallego-Bartolomé
    •  & Steven E. Jacobsen
  • Article | | open

    Abandoned and degraded agricultural lands undergo ecological succession that sequesters atmospheric CO2 as soil carbon, but at low rates. Here the authors show that restoration of high plant diversity provides a greenhouse gas benefit by greatly increasing the rate of soil carbon sequestration on such lands.

    • Yi Yang
    • , David Tilman
    • , George Furey
    •  & Clarence Lehman
  • Article | | open

    Oxidation of magnetite has broad implications in geochemistry and environmental science, but its reaction mechanisms are not fully understood yet. Here the authors use Bragg coherent diffractive imaging to show oxidative dissolution of magnetite inducing a rich array of strain and defect structures.

    • Ke Yuan
    • , Sang Soo Lee
    • , Wonsuk Cha
    • , Andrew Ulvestad
    • , Hyunjung Kim
    • , Bektur Abdilla
    • , Neil C. Sturchio
    •  & Paul Fenter
  • Article | | open

    There has been a lack of multi-year landscape-scale studies on the effect of neonicotinoids on honeybee health. Here, Osterman et al. show that clothianidin exposure via seed-treated rapeseed has no negative impact on honeybee colony development, microbial pathogens/symbionts or immune gene expression.

    • Julia Osterman
    • , Dimitry Wintermantel
    • , Barbara Locke
    • , Ove Jonsson
    • , Emilia Semberg
    • , Piero Onorati
    • , Eva Forsgren
    • , Peter Rosenkranz
    • , Thorsten Rahbek-Pedersen
    • , Riccardo Bommarco
    • , Henrik G. Smith
    • , Maj Rundlöf
    •  & Joachim R. de Miranda
  • Article | | open

    How Japanese encephalitis virus (JEV) penetrates the blood-brain barrier (BBB) remains unclear. Here, using a genetic mouse model and a virulent JEV strain, the authors show that perivascular mast cells (MC) mediate JEV neuroinvasion and identify the MC-protease chymase as a potential therapeutic target.

    • Justin T. Hsieh
    • , Abhay P. S. Rathore
    • , Gayathri Soundarajan
    •  & Ashley L. St. John
  • Article | | open

    Fire-derived organic matter (OM) is present throughout the environment, and its impact on nutrient cycling remains poorly understood. Here, the authors show that this pyrogenic OM can retain large quantities of ammonia through covalent bond formation, thereby exerting an important control on nitrogen cycling.

    • Rachel Hestrin
    • , Dorisel Torres-Rojas
    • , James J. Dynes
    • , James M. Hook
    • , Tom Z. Regier
    • , Adam W. Gillespie
    • , Ronald J. Smernik
    •  & Johannes Lehmann
  • Article | | open

    Corticosteroids are host-directed drugs that enhance survival of tuberculosis patients through unclear mechanisms. Here, Gräb et al. show that corticosteroids inhibit necrotic death of cells infected with Mycobacterium tuberculosis by facilitating MKP-1-dependent dephosphorylation of p38 MAPK.

    • Jessica Gräb
    • , Isabelle Suárez
    • , Edeltraud van Gumpel
    • , Sandra Winter
    • , Fynn Schreiber
    • , Anna Esser
    • , Christoph Hölscher
    • , Melanie Fritsch
    • , Marc Herb
    • , Michael Schramm
    • , Laurens Wachsmuth
    • , Christian Pallasch
    • , Manolis Pasparakis
    • , Hamid Kashkar
    •  & Jan Rybniker
  • Article | | open

    Many Gram-negative bacteria rely on a type III secretion system (T3SS) for their pathogenicity. Here authors present the cryo-EM structure of the E.coli T3SS ATPase-central stalk complex, which forms a homohexameric, asymmetric pore with different functional states.

    • Dorothy D. Majewski
    • , Liam J. Worrall
    • , Chuan Hong
    • , Claire E. Atkinson
    • , Marija Vuckovic
    • , Nobuhiko Watanabe
    • , Zhiheng Yu
    •  & Natalie C. J. Strynadka
  • Article | | open

    The pathogen Pseudomonas aeruginosa undergoes complex trait adaptation within cystic fibrosis patients. Here, Bartell, Sommer, and colleagues use statistical modeling of longitudinal isolates to characterize the joint genetic and phenotypic evolutionary trajectories of P. aeruginosa within hosts.

    • Jennifer A. Bartell
    • , Lea M. Sommer
    • , Janus A. J. Haagensen
    • , Anne Loch
    • , Rocio Espinosa
    • , Søren Molin
    •  & Helle Krogh Johansen
  • Article | | open

    Development of bacterial resistance to an antibiotic can lead to collateral sensitivity to another drug. Here, the authors study collateral sensitivity conferred by mutations in the horizontally acquired β-lactamase CTX-M-15, and identify antibiotic combinations that constrain the evolution of resistance.

    • Carola E. H. Rosenkilde
    • , Christian Munck
    • , Andreas Porse
    • , Marius Linkevicius
    • , Dan I. Andersson
    •  & Morten O. A. Sommer
  • Article | | open

    Ferromanganese minerals are abundant in marine environments but the extent of these minerals in subseafloor sediments remains unknown. Here the authors find abundant ferromanganese microparticles in oxic pelagic clays, accounting for 14–16% of the new estimate of the global manganese budget (9.2–47.4 Tt).

    • Go-Ichiro Uramoto
    • , Yuki Morono
    • , Naotaka Tomioka
    • , Shigeyuki Wakaki
    • , Ryoichi Nakada
    • , Rota Wagai
    • , Kentaro Uesugi
    • , Akihisa Takeuchi
    • , Masato Hoshino
    • , Yoshio Suzuki
    • , Fumito Shiraishi
    • , Satoshi Mitsunobu
    • , Hiroki Suga
    • , Yasuo Takeichi
    • , Yoshio Takahashi
    •  & Fumio Inagaki
  • Article | | open

    Host genetics modulates gut microbiota composition, which in turn affects host health, but little is known about the specific genes involved. Here, Berg et al. show that TGFβ/BMP immune signalling affects abundance and function of commensal Enterobacter species in C. elegans.

    • Maureen Berg
    • , David Monnin
    • , Juhyun Cho
    • , Lydia Nelson
    • , Alex Crits-Christoph
    •  & Michael Shapira
  • Article | | open

    Antibodies against Plasmodium falciparum merozoites that fix complement can inhibit blood-stage replication. Here, Reiling et al. show that complement-fixing antibodies strongly correlate with protective immunity in children, identify the merozoite targets, and predict antigen combinations that should result in strong protection.

    • Linda Reiling
    • , Michelle J. Boyle
    • , Michael T. White
    • , Danny W. Wilson
    • , Gaoqian Feng
    • , Rupert Weaver
    • , D. Herbert Opi
    • , Kristina E. M. Persson
    • , Jack S. Richards
    • , Peter M. Siba
    • , Freya J. I. Fowkes
    • , Eizo Takashima
    • , Takafumi Tsuboi
    • , Ivo Mueller
    •  & James G. Beeson
  • Article | | open

    Gene transfer agents (GTAs) are ‘domesticated’ bacteriophages that can transfer any genes between bacteria. Here, Paul Fogg identifies a protein that directly regulates transcription of GTA genes and whose expression is in turn controlled by a global cell-cycle regulator and a quorum-sensing regulator.

    • Paul C. M. Fogg
  • Article | | open

    End-product toxicity, culture contamination, and energy efficient product recovery are long-standing issues in bioprocessing. Here, the authors address these problems using a fermentation strategy that combines microbial production of branched alcohols with supercritical carbon dioxide extraction.

    • Jason T. Boock
    • , Adam J. E. Freedman
    • , Geoffrey A. Tompsett
    • , Sarah K. Muse
    • , Audrey J. Allen
    • , Luke A. Jackson
    • , Bernardo Castro-Dominguez
    • , Michael T. Timko
    • , Kristala L. J. Prather
    •  & Janelle R. Thompson
  • Article | | open

    N6-methyladenine is involved in many biological pathways for microbial survival and host interaction. Here the authors train a neural network for improved m6A detection in nanopore sequencing data and validate methylomes for a microbial reference community.

    • Alexa B. R. McIntyre
    • , Noah Alexander
    • , Kirill Grigorev
    • , Daniela Bezdan
    • , Heike Sichtig
    • , Charles Y. Chiu
    •  & Christopher E. Mason
  • Article | | open

    The genomic locations that can be targeted for editing by CRISPR are limited by the presence of the nuclease-specific PAM sequence. Here, the authors show PAM recognition can be expanded by replacing the key region in the PAM interaction domain of SaCas9 with the corresponding region of SaCas9 orthologs.

    • Dacheng Ma
    • , Zhimeng Xu
    • , Zhaoyu Zhang
    • , Xi Chen
    • , Xiangzhi Zeng
    • , Yiyang Zhang
    • , Tingyue Deng
    • , Mengfei Ren
    • , Zheng Sun
    • , Rui Jiang
    •  & Zhen Xie
  • Article | | open

    Producing individual cannabinoids by metabolically engineered microbes has proven challenging. Here, the authors develop a cell-free enzymatic prenylating system to generate isoprenyl pyrophosphate substrates directly from glucose and produce both common and rare cannabinoids at >1 g/L.

    • Meaghan A. Valliere
    • , Tyler P. Korman
    • , Nicholas B. Woodall
    • , Gregory A. Khitrov
    • , Robert E. Taylor
    • , David Baker
    •  & James U. Bowie
  • Article | | open

    Recent efforts have been made to apply ecological theory on succession to understand the dynamics of human microbiomes throughout development. Here, Guittar et al. use a trait-based approach to show how microbial traits putatively related to dispersal and environmental tolerance shift in the infant microbiome over the first three years of life.

    • John Guittar
    • , Ashley Shade
    •  & Elena Litchman
  • Article | | open

    Previous surveys of global ocean microbial diversity have focused on planktonic microbes. Here, Zhang et al. use metagenomics to study biofilm-forming marine microbes, increasing the known microbial diversity in the oceans by more than 20% and revealing new biosynthetic gene clusters and CRISPR-Cas systems.

    • Weipeng Zhang
    • , Wei Ding
    • , Yong-Xin Li
    • , Chunkit Tam
    • , Salim Bougouffa
    • , Ruojun Wang
    • , Bite Pei
    • , Hoyin Chiang
    • , Pokman Leung
    • , Yanhong Lu
    • , Jin Sun
    • , He Fu
    • , Vladimir B Bajic
    • , Hongbin Liu
    • , Nicole S. Webster
    •  & Pei-Yuan Qian
  • Article | | open

    Host microbiomes are feasible sources for drug discovery. Here, using large-scale isolations, bioactivity assays and omics, the authors uncover the antimicrobial potential of insect-associated Streptomyces and identify a compound, cyphomycin, active against multidrug-resistant fungal pathogens.

    • Marc G. Chevrette
    • , Caitlin M. Carlson
    • , Humberto E. Ortega
    • , Chris Thomas
    • , Gene E. Ananiev
    • , Kenneth J. Barns
    • , Adam J. Book
    • , Julian Cagnazzo
    • , Camila Carlos
    • , Will Flanigan
    • , Kirk J. Grubbs
    • , Heidi A. Horn
    • , F. Michael Hoffmann
    • , Jonathan L. Klassen
    • , Jennifer J. Knack
    • , Gina R. Lewin
    • , Bradon R. McDonald
    • , Laura Muller
    • , Weilan G. P. Melo
    • , Adrián A. Pinto-Tomás
    • , Amber Schmitz
    • , Evelyn Wendt-Pienkowski
    • , Scott Wildman
    • , Miao Zhao
    • , Fan Zhang
    • , Tim S. Bugni
    • , David R. Andes
    • , Monica T. Pupo
    •  & Cameron R. Currie
  • Article | | open

    Combination therapy for Buruli ulcer (BU) is suboptimal. Here, Liu et al. show that the candidate drug TB47 has potent bactericidal activity against Mycobacterium ulcerans in vitro and in a mouse model, which underscores its potential for shortening the course of BU and treating other mycobacterial diseases.

    • Yang Liu
    • , Yamin Gao
    • , Jianxiong Liu
    • , Yaoju Tan
    • , Zhiyong Liu
    • , Chiranjibi Chhotaray
    • , Huofeng Jiang
    • , Zhili Lu
    • , Gift Chiwala
    • , Shuai Wang
    • , Gaelle Makafe
    • , Md Mahmudul Islam
    • , H. M. Adnan Hameed
    • , Xingshan Cai
    • , Changwei Wang
    • , Xinjie Li
    • , Shouyong Tan
    •  & Tianyu Zhang
  • Article | | open

    Host-parasite interactions during the exoerythrocytic stage of Plasmodium infection remains poorly understood. Using dual RNA-Seq, the authors show that human mucosal immunity protein mucin-13 is upregulated during Plasmodium hepatic-stage infection and marks infected cells independent of tested Plasmodium species.

    • Gregory M. LaMonte
    • , Pamela Orjuela-Sanchez
    • , Jaeson Calla
    • , Lawrence T. Wang
    • , Shangzhong Li
    • , Justine Swann
    • , Annie N. Cowell
    • , Bing Yu Zou
    • , Alyaa M. Abdel-Haleem Mohamed
    • , Zaira Hellen Villa Galarce
    • , Marta Moreno
    • , Carlos Tong Rios
    • , Joseph M. Vinetz
    • , Nathan Lewis
    •  & Elizabeth A. Winzeler
  • Article | | open

    It remains unclear when and why the world’s oceans, once largely occupied by bacteria, became dominated by photosynthetic algae. Here, using fossil lipids in million year old rocks, the authors show that predation after the Snowball Earth glaciations created the opportunity for a global shift to algal ecosystems.

    • Lennart M. van Maldegem
    • , Pierre Sansjofre
    • , Johan W. H. Weijers
    • , Klaus Wolkenstein
    • , Paul K. Strother
    • , Lars Wörmer
    • , Jens Hefter
    • , Benjamin J. Nettersheim
    • , Yosuke Hoshino
    • , Stefan Schouten
    • , Jaap S. Sinninghe Damsté
    • , Nilamoni Nath
    • , Christian Griesinger
    • , Nikolay B. Kuznetsov
    • , Marcel Elie
    • , Marcus Elvert
    • , Erik Tegelaar
    • , Gerd Gleixner
    •  & Christian Hallmann
  • Article | | open

    Organs from donors with transmissible viral infections, such as hepatitis C virus (HCV), are not offered for transplantation due to a high risk of transmission. Here, Galasso et al. develop a method for treatment of HCV-infected human donor lungs that is safe and prevents HCV transmission in the pig model.

    • Marcos Galasso
    • , Jordan J. Feld
    • , Yui Watanabe
    • , Mauricio Pipkin
    • , Cara Summers
    • , Aadil Ali
    • , Robert Qaqish
    • , Manyin Chen
    • , Rafaela V. P. Ribeiro
    • , Khaled Ramadan
    • , Layla Pires
    • , Vanderlei S. Bagnato
    • , Cristina Kurachi
    • , Vera Cherepanov
    • , Gray Moonen
    • , Anajara Gazzalle
    • , Thomas K. Waddell
    • , Mingyao Liu
    • , Shaf Keshavjee
    • , Brian C. Wilson
    • , Atul Humar
    •  & Marcelo Cypel
  • Article | | open

    The underlying mechanisms structuring dissolved organic matter (DOM) composition and reactivity in rivers remain poorly quantified. Here, the authors pair mass spectrometry and fluorescence spectroscopy to show that hydrology and river geomorphology both shape molecular patterns in DOM composition.

    • Laurel M. Lynch
    • , Nicholas A. Sutfin
    • , Timothy S. Fegel
    • , Claudia M. Boot
    • , Timothy P. Covino
    •  & Matthew D. Wallenstein
  • Article | | open

    Most cyanobacteria are oxygenic photoautotrophs, and fermenters under dark anoxic conditions. Here, the authors analyse genomic sequences of related uncultivated bacteria, inferring their metabolic potential, and supporting that their common ancestor was an anaerobe capable of fermentation and H2 metabolism.

    • Paula B. Matheus Carnevali
    • , Frederik Schulz
    • , Cindy J. Castelle
    • , Rose S. Kantor
    • , Patrick M. Shih
    • , Itai Sharon
    • , Joanne M. Santini
    • , Matthew R. Olm
    • , Yuki Amano
    • , Brian C. Thomas
    • , Karthik Anantharaman
    • , David Burstein
    • , Eric D. Becraft
    • , Ramunas Stepanauskas
    • , Tanja Woyke
    •  & Jillian F. Banfield
  • Article | | open

    Obesity is associated with altered N-acylethanolamine levels (NAE). Here the authors show that deletion of the gene encoding N-acylphosphatidylethanolamine phospholipase D, a key enzyme for NAE synthesis, in intestinal cells of mice leads to the development of obesity and hepatic steatosis via a mechanism involving the gut-brain axis.

    • Amandine Everard
    • , Hubert Plovier
    • , Marialetizia Rastelli
    • , Matthias Van Hul
    • , Alice de Wouters d’Oplinter
    • , Lucie Geurts
    • , Céline Druart
    • , Sylvie Robine
    • , Nathalie M. Delzenne
    • , Giulio G. Muccioli
    • , Willem M. de Vos
    • , Serge Luquet
    • , Nicolas Flamand
    • , Vincenzo Di Marzo
    •  & Patrice D. Cani
  • Article | | open

    Multiple morphological abnormalities of the sperm flagella (MMAF) is a cause of male infertility. Here the authors identify homozygous nonsense mutations of the glutamine rich 2 (QRICH2) gene in two MMAF patients from 2 consanguineous families and show using QRICH2 knockout mice that the protein is required for sperm flagellar formation and motility.

    • Ying Shen
    • , Feng Zhang
    • , Fuping Li
    • , Xiaohui Jiang
    • , Yihong Yang
    • , Xiaoliang Li
    • , Weiyu Li
    • , Xiang Wang
    • , Juan Cheng
    • , Mohan Liu
    • , Xueguang Zhang
    • , Guiping Yuan
    • , Xue Pei
    • , Kailai Cai
    • , Fengyun Hu
    • , Jianfeng Sun
    • , Lanzhen Yan
    • , Li Tang
    • , Chuan Jiang
    • , Wenling Tu
    • , Jinyan Xu
    • , Haojuan Wu
    • , Weiqi Kong
    • , Shuying Li
    • , Ke Wang
    • , Kai Sheng
    • , Xudong Zhao
    • , Huanxun Yue
    • , Xiaoyu Yang
    •  & Wenming Xu
  • Article | | open

    The structure and distribution of strain-level diversity in host-associated bacterial communities is largely unexplored. Here, Ellegaard and Engel analyze strain level diversity of the honey bee gut microbiota, showing that bees from the same colony differ in strain but not phylotype composition.

    • Kirsten M. Ellegaard
    •  & Philipp Engel
  • Article | | open

    Bacterial cellulose (BC) is a ubiquitous biomaterial, but functionalization relies on modification processes which have low reaction efficiency and can cause pollution. Here the authors develop an environmental friendly in-situ fermentation method using fluorescein labelled glucose to produce fluorescent BC.

    • Minghong Gao
    • , Juan Li
    • , Zixian Bao
    • , Moudong Hu
    • , Rui Nian
    • , Dexin Feng
    • , Dong An
    • , Xing Li
    • , Mo Xian
    •  & Haibo Zhang
  • Article | | open

    The chaperone Hsp90 is a potential target for the development of drugs against fungal pathogens. Here the authors determine the structure of the Hsp90 nucleotide-binding domain from Candida albicans, which they use to design an inhibitor and demonstrate its selectivity for the fungal enzyme, both biochemically and in cells.

    • Luke Whitesell
    • , Nicole Robbins
    • , David S. Huang
    • , Catherine A. McLellan
    • , Tanvi Shekhar-Guturja
    • , Emmanuelle V. LeBlanc
    • , Catherine S. Nation
    • , Raymond Hui
    • , Ashley Hutchinson
    • , Cathy Collins
    • , Sharanya Chatterjee
    • , Richard Trilles
    • , Jinglin L. Xie
    • , Damian J. Krysan
    • , Susan Lindquist
    • , John A. Porco Jr.
    • , Utpal Tatu
    • , Lauren E. Brown
    • , Juan Pizarro
    •  & Leah E. Cowen
  • Article | | open

    Nucleocytoplasmic large DNA viruses are a group of viruses that infect many eukaryotic hosts. Here, the authors provide a 3.5 Å resolution icosahedrally-averaged capsid structure of Paramecium bursaria chlorella virus 1 and show how 1800 minor capsid proteins form a hexagonal network below the outer capsid shell.

    • Qianglin Fang
    • , Dongjie Zhu
    • , Irina Agarkova
    • , Jagat Adhikari
    • , Thomas Klose
    • , Yue Liu
    • , Zhenguo Chen
    • , Yingyuan Sun
    • , Michael L. Gross
    • , James L. Van Etten
    • , Xinzheng Zhang
    •  & Michael G. Rossmann
  • Article | | open

    The DNA ligase of African swine fever virus is one of the most error-prone ligases identified to date, but underlying molecular details are lacking. Here, Chen et al. report four AsfvLIG:DNA structures and identify a unique N-terminal domain and four unique active site residues that are crucial for its catalytic efficiency.

    • Yiqing Chen
    • , Hehua Liu
    • , Chun Yang
    • , Yanqing Gao
    • , Xiang Yu
    • , Xi Chen
    • , Ruixue Cui
    • , Lina Zheng
    • , Suhua Li
    • , Xuhang Li
    • , Jinbiao Ma
    • , Zhen Huang
    • , Jixi Li
    •  & Jianhua Gan