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Featured
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Research Highlights |
Seeing DNA
DNA and chromatin structures can be visualized in situ with electron tomography.
- Zachary J Lapin
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Methods in Brief |
FAST imaging of whole brains
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Research Highlights |
Gold glitters in transparent tissue
Through scattering, in situ metal particle growth enables biomolecule visualization within tissue.
- Zachary J Lapin
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Methods in Brief |
Speckle-free OCT
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Research Highlights |
Tailoring optogenetic illumination through tapered fibers
A tapered optical fiber delivers spatially precise and efficient optogenetic illumination to the rodent brain.
- Nina Vogt
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Methods in Brief |
Reconciling small and large scales with FIB-SEM
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Research Highlights |
Zooming into the larval zebrafish brain
A serial-section electron microscopy data set of larval zebrafish brain—imaged at several scales—provides a resource for structure–function analyses of the animals' neural circuitry.
- Nina Vogt
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This Month |
Chris Xu
Deep brain imaging with three-photon microscopy and a swim from the US to China.
- Vivien Marx
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Methods in Brief |
High-speed imaging of neural activity in behaving animals
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Methods in Brief |
Optoacoustic imaging at multiple spatiotemporal scales
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Quantitative analysis tools and correlative imaging applications for N-STORM
- Lynne Chang
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Screening and quantification of the tumor microenvironment with micro-ultrasound and photoacoustic imaging
- Minalini Lakshman
- & Andrew Needles
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Article |
Whole-brain functional imaging at cellular resolution using light-sheet microscopy
Whole-brain imaging of neuronal activity with cellular resolution at almost a brain per second is demonstrated using high-speed light-sheet microscopy in the larval zebrafish brain.
- Misha B Ahrens
- , Michael B Orger
- & Philipp J Keller
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Brief Communication |
Serial two-photon tomography for automated ex vivo mouse brain imaging
Automated tissue sectioning and two-photon imaging of fluorescently labeled and fixed mouse brains allows high-resolution tomographic imaging of the entire brain. The authors demonstrate performance using multiple GFP mouse lines, dye-based retrograde tracing and viral anterograde tracing.
- Timothy Ragan
- , Lolahon R Kadiri
- & Pavel Osten
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Research Highlights |
Clarifying brain structure, literally
A fluorescence-compatible tissue-clearing reagent enables light microscopy–based imaging deep in the mouse brain.
- Petya V Krasteva
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This Month |
Abbas El Gamal and Mark Schnitzer
Two-gram microscopes make brain images in moving mice.
- Monya Baker
- , Abbas El Gamal
- & Mark Schnitzer
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Article |
Miniaturized integration of a fluorescence microscope
An integrated, miniature (1.9 g) fluorescence microscope containing light source, optics and sensor allows high-speed, wide field of view imaging of calcium spiking in hundreds of neurons in freely moving mice. The mass-producible portable microscope is also useful for a variety of fluorescence assays for which size, cost and portability can be concerns.
- Kunal K Ghosh
- , Laurie D Burns
- & Mark J Schnitzer
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News & Views |
From journal articles to computational models: a new automated tool
Automated methods can now extract brain-image coordinates appearing in hundreds of publications in targeted topic areas and then integrate these data to form computational models that classify new brain-image data.
- Tom M. Mitchell
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Brief Communication |
Functional ultrasound imaging of the brain
A new method called functional ultrasound (fUS) is reported that allows imaging of transient changes in blood volume in the whole rat brain with a spatiotemporal resolution not attained by other functional brain imaging modalities.
- Emilie Macé
- , Gabriel Montaldo
- & Mickael Tanter
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Research Highlights |
Brain function marries anatomy
Researchers have taken first steps toward functional connectomics. By combining large-scale serial electron microscopy and functional imaging data, the structure of neural networks can be related to their function.
- Erika Pastrana
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News & Views |
Functional whole-brain imaging in behaving rodents
A wearable imaging device allows functional whole-brain imaging of awake, freely moving rats. This technology opens up a noninvasive window for simultaneously assessing brain function and behavior in response to a wide variety of interventions in living rats.
- Simon R Cherry
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Research Highlights |
Unraveling synapse diversity
Array tomography opens the door to the large-scale exploration of molecular diversity of individual brain synapses.
- Erika Pastrana
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Editorial |
Brain observations
New tools are improving the prospects for transcranial light-based neuroscience, but better methods for using them are needed before they can reach their full potential.
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Article |
Two-photon high-resolution measurement of partial pressure of oxygen in cerebral vasculature and tissue
Two-photon excitation of a phosphorescent nanoprobe that is quenched by molecular oxygen permits high-resolution measurements of oxygen in both the vasculature and tissue of rodent brain.
- Sava Sakadžić
- , Emmanuel Roussakis
- & David A Boas
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This Month |
Thomas Knöpfel
Linked fluorescent proteins are used to visualize voltage in living mouse brains.
- Monya Baker
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Article |
Imaging brain electric signals with genetically targeted voltage-sensitive fluorescent proteins
Genetically encoded voltage-sensitive fluorescent proteins can be used to measure electrical activity from selected populations of neurons. This study demonstrates that these probes, when expressed in pyramidal cells of mouse somatosensory cortex, can report electrical responses in vivo. These proteins are a complementary tool to calcium imaging techniques for optical functional brain imaging.
- Walther Akemann
- , Hiroki Mutoh
- & Thomas Knöpfel
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Research Highlights |
Connecting the dots in 3D
New software tools help take the pain out of working with huge three-dimensional image datasets and aid in mapping neuronal networks.
- Daniel Evanko
The new 2D Superresolution mode for ZEISS Airyscan