Immunohistochemistry articles within Nature Communications

Featured

  • Article
    | Open Access

    The mechanisms behind the negative effects of social isolation on social species are unclear. Here, the authors examine colonies of carpenter ants, finding that behavioral, physiological, and lifespan changes may be caused by oxidative stress.

    • Akiko Koto
    • , Makoto Tamura
    •  & Laurent Keller

  • Article
    | Open Access

    Current single-cell tools are limited by the number of proteins they can analyse. Here the authors report a single-cell cyclic multiplex in situ tagging (CycMIST) method for functional proteome profiling of single cells, allowing multiple rounds of multiplexing of the same single cells on a microchip.

    • Liwei Yang
    • , Avery Ball
    •  & Jun Wang

  • Article
    | Open Access

    Ultra-multiplexed fluorescence imaging is currently difficult. Here the authors report PICASSO which enables 15-colour imaging of spatially overlapping proteins in a single-round of imaging; they combine it with cyclic immunofluorescence to achieve 45-colour imaging of the mouse brain in 3 cycles.

    • Junyoung Seo
    • , Yeonbo Sim
    •  & Jae-Byum Chang

  • Article
    | Open Access

    Mapping neuroanatomy is a foundational goal of connectomics, and the gold standard method is electron microscopy as light microscopy lacks nanoscale resolution. Here the authors develop a strategy using multicolor genetic labeling (Brainbow) and expansion microscopy to map putative synaptic connections using light microscopy.

    • Fred Y. Shen
    • , Margaret M. Harrington
    •  & Dawen Cai

  • Article
    | Open Access

    Oral cancer has region-specific histopathological and molecular characteristics, complicating its classification by the standard tumor-node-metastasis system. Here, the authors combine discovery and targeted proteomics with IHC to identify region-specific and saliva biomarkers for oral cancer prognosis.

    • Carolina Moretto Carnielli
    • , Carolina Carneiro Soares Macedo
    •  & Adriana Franco Paes Leme

  • Article
    | Open Access

    A large body of work into the role of oestrogen receptor b (ERb) in breast cancer is contradictory, hindering future progress. Here the authors conduct extensive validation of anti-ERb antibodies , and show that normal and cancerous breast tissue do not express ERb, consistent with RNA-seq data.

    • Sandra Andersson
    • , Mårten Sundberg
    •  & Anna Asplund

  • Article
    | Open Access

    The ability to measure signalling responses in single cells following short pulses of stimulus would shed insight into temporal thresholds for cell activation. Here the authors introduce a microfluidic platform that allows downstream phosphorylation cascades to be observed following as little as one second of stimulus exposure.

    • Alphonsus H. C. Ng
    • , M. Dean Chamberlain
    •  & Aaron R. Wheeler

  • Article |

    Imaging cells within live animals is important in biomedical research, but this process is limited by the availability of probes. Ritsma et al.combine intravital microscopy with immunohistochemistry to increase the panel of available reagents and examine the role of T cells in the migration of breast cancer cells.

    • Laila Ritsma
    • , Nienke Vrisekoop
    •  & Jacco van Rheenen

Browse broader subjects

Browse Immunohistochemistry across other nature.com journals