Immunoblotting articles within Nature Communications

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  • Article
    | Open Access

    Recombinant proteins in complex solutions are typically detected with tag-specific antibodies in Western blots. Here, the author describes an antibody-free alternative in which tagged proteins are detected directly in polyacrylamide gels via fluorophore-labelling of the tagged protein using a ligase.

    • Adrian C. D. Fuchs

  • Article
    | Open Access

    The mode of cytokine receptor activation is diverse. Here, the authors find that the marine-sponge derived lectin ThC, a bivalent sugar binding protein, activates human cytokine receptor MPL. This mode of action resembles the pathogenic activation of MPL by mutant molecular chaperon calreticulin in hematologic malignancies.

    • Hiromi Watari
    • , Hiromu Kageyama
    •  & Ryuichi Sakai

  • Article
    | Open Access

    Circulating tumour cells (CTCs) are rare cells found in the blood of certain cancer patients. Here, the authors develop a cytometry tool that appends a microfluidic western blot to a CTC isolation workflow and apply it to profile a panel of proteins in single CTCs isolated from ER+ breast cancer patients.

    • Elly Sinkala
    • , Elodie Sollier-Christen
    •  & Amy E. Herr

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