Featured
-
-
Article
| Open AccessDNA methylation in mice is influenced by genetics as well as sex and life experience
DNA methylation is an epigenetic mark involved in gene regulation. Here the authors investigate the extent to which genetics, sex and pregnancy influence genomic DNA methylation in mice, providing evidence of the stability of CpG methylation across generation and suggest that CpG methylation may serve as an epigenetic record of life events in somatic tissues at loci whose expression is linked to the relevant biology.
- Sara A. Grimm
- , Takashi Shimbo
- & Paul A. Wade
-
Article
| Open AccessBiological relevance of computationally predicted pathogenicity of noncoding variants
Researchers can make use of a variety of computational tools to prioritize genetic variants and predict their pathogenicity. Here, the authors evaluate the performance of six of these tools in three typical biological tasks and find generally low concordance of predictions and experimental confirmation.
- Li Liu
- , Maxwell D. Sanderford
- & Sudhir Kumar
-
Article
| Open AccessApparent latent structure within the UK Biobank sample has implications for epidemiological analysis
Population structure can bias the results of genetic and epidemiological analysis. Here, Haworth et al. report that fine-scale structure is detectable in apparently homogeneous samples such as ALSPAC when measured very precisely, and remains detectable in UK Biobank despite conventional approaches to account for it.
- Simon Haworth
- , Ruth Mitchell
- & Nicholas J. Timpson
-
Article
| Open AccessAberrant enhancer hypomethylation contributes to hepatic carcinogenesis through global transcriptional reprogramming
There are distinct hypermethylation patterns in gene promoters in hepatocellular carcinomas (HCCs). Here, the authors show that the enhancer of C/EBPβ is recurrently hypomethylated in human HCCs, recapitulating this in a transgenic murine model and linking aberrant enhancer hypomethylation to hepatocarcinogenesis.
- Lei Xiong
- , Feng Wu
- & Ka-Fai To
-
Article
| Open AccessDual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria
Gain of function methods based on gene overexpression are not easily applied to high-throughput screening across different experimental conditions. Here, the authors present Dub-seq, which separates overexpression library characterization from functional screening and uses random DNA barcodes to increase the experimental throughput.
- Vivek K. Mutalik
- , Pavel S. Novichkov
- & Adam P. Arkin
-
Article
| Open AccessE47 modulates hepatic glucocorticoid action
Glucocorticoids (GCs) are widely used anti-inflammatory drugs; however, long-term treatment causes metabolic side effects. Here, the authors show that E47 is a modulator of glucocorticoid receptor activity for a subset of target genes in mouse liver, and that loss of E47 protects mice from hyperglycemia and hepatic steatosis in response to GCs.
- M. Charlotte Hemmer
- , Michael Wierer
- & N. Henriette Uhlenhaut
-
Article
| Open AccessChromosome-level assembly of the water buffalo genome surpasses human and goat genomes in sequence contiguity
Despite technological advances, chromosome-level assemblies of mammalian genomes are still rare. Here, the authors use PacBio, Chicago and Hi-C approaches to generate a highly contiguous and partially-phased genome assembly for the water buffalo, Bubalus bubalis
- Wai Yee Low
- , Rick Tearle
- & John L. Williams
-
Article
| Open AccessApproximate Bayesian computation with deep learning supports a third archaic introgression in Asia and Oceania
Introgression of Neanderthals and Denisovans left genomic signals in anatomically modern human after Out-of-Africa event. Here, the authors identify a third archaic introgression common to all Asian and Oceanian human populations by applying an approximate Bayesian computation with a Deep Learning framework.
- Mayukh Mondal
- , Jaume Bertranpetit
- & Oscar Lao
-
Article
| Open AccessA conserved dimer interface connects ERH and YTH family proteins to promote gene silencing
In fission yeast, Erh1, ortholog of human ERH, interacts with the YTH family RNA binding protein Mmi1 to form the Erh1-Mmi1 complex (EMC), which has been implicated in gene silencing. Here, the authors present the cocrystal structure of Erh1 homodimers interacting with Mmi1 and further characterise the role of EMC in facultative heterochromatin assembly and gene silencing.
- Guodong Xie
- , Tommy V. Vo
- & Shiv I. S. Grewal
-
Article
| Open AccessSupplementary stocking selects for domesticated genotypes
Stocking of hatchery produced fish is widely used to supplement wild fish populations. Here, the authors show that supplementary stocking can unintentionally favour introgressed individuals with domestic genotypes and compromise the fitness of a wild population of Atlantic salmon.
- Ingerid J. Hagen
- , Arne J. Jensen
- & Sten Karlsson
-
Article
| Open AccessIntergenerational inheritance of high fat diet-induced cardiac lipotoxicity in Drosophila
Animal studies have shown that the nutritional status of parents can predispose the offspring to obesity and obesity-related diseases. Here the authors show that cardiac dysfunction induced by a high-fat diet persists for two generations in Drosophila, and that targeted expression of ATGL/bmm in the offspring, as well as inhibition of H3K27 trimethylation, is cardioprotective.
- Maria Clara Guida
- , Ryan Tyge Birse
- & Rolf Bodmer
-
Article
| Open AccessGenome-wide association analyses of invasive pneumococcal isolates identify a missense bacterial mutation associated with meningitis
Meningitis is a severe form of invasive pneumococcal disease (IPD). To study the contribution of bacterial genomic variation, here Li et al. perform whole genome sequencing of pneumococcal isolates from IPD patients and identify an association for higher risk of meningitis with a pbp1bA641C variant
- Yuan Li
- , Benjamin J. Metcalf
- & Bernard W. Beall
-
Article
| Open AccessThe evolution of sex determination associated with a chromosomal inversion
Turnovers in sex determination systems occur quite frequently, yet the evolutionary drivers of these turnovers are not well understood. Here, the authors study the sex determination systems in sticklebacks and propose chromosomal inversions as a possible driver of the evolution of sex determination.
- Heini M. Natri
- , Juha Merilä
- & Takahito Shikano
-
Article
| Open AccessPartial proteasomal degradation of Lola triggers the male-to-female switch of a dimorphic courtship circuit
It is unclear how some Drosophila neurons develop sex-specific neurites that contribute to dimorphic circuitries required for gendered behavior. The authors show that sex-specific cleavage by the E3 ubiquitin ligase Cullin1 and 26S proteasome of the pleiotropic BTB-ZF transcription factor Lola confers its sexual fate-inducing ability in these neurons.
- Kosei Sato
- , Hiroki Ito
- & Daisuke Yamamoto
-
Article
| Open AccessSingle-cell microRNA-mRNA co-sequencing reveals non-genetic heterogeneity and mechanisms of microRNA regulation
Single-cell RNA sequencing allows characterizing cell-to-cell heterogeneity at transcriptome scale. Here, the authors present an approach that enables microRNA and mRNA sequencing in the same single cell, providing insights into the origins of non-genetic cellular variability and mechanisms of miRNA regulation.
- Nayi Wang
- , Ji Zheng
- & Jun Lu
-
Article
| Open AccessTransforming insect population control with precision guided sterile males with demonstration in flies
Sterile Insect Technique (SIT) is used to suppress wild populations. Here the authors integrate CRISPR-based technology and SIT to develop a precision guided SIT (pgSIT), and demonstrate its proof-of-principle by generating 100% sterile males.
- Nikolay P. Kandul
- , Junru Liu
- & Omar S. Akbari
-
Article
| Open AccessEmergence of a floral colour polymorphism by pollinator-mediated overdominance
Examples of overdominance are usually explained by deleterious effects in homozygotes. Here, Kellenberger et al. describe a case of overdominance in the floral color of the Alpine orchid Gymnadenia rhellicani apparently maintained by pollinator preferences without deleterious effects in homozygotes.
- Roman T. Kellenberger
- , Kelsey J. R. P. Byers
- & Philipp M. Schlüter
-
Article
| Open AccessATM orchestrates the DNA-damage response to counter toxic non-homologous end-joining at broken replication forks
Mutations in the ATM tumor suppressor gene confer hypersensitivity to DNA-damaging chemotherapeutic agents. Here, the authors provide evidence that these hypersensitivities reflect a crucial role for ATM at damaged replication forks being to prevent toxic DNA end-joining leading to chromosome fusions and cell death.
- Gabriel Balmus
- , Domenic Pilger
- & Stephen P. Jackson
-
Article
| Open AccessGenome-wide profiling of adenine base editor specificity by EndoV-seq
Adenine base editors are an important contribution to the genome editing toolbox. Here the authors present EndoV-seq, an endonuclease-based assay for evaluating genomewide off-target effects of base editing.
- Puping Liang
- , Xiaowei Xie
- & Zhou Songyang
-
Article
| Open AccessLongevity defined as top 10% survivors and beyond is transmitted as a quantitative genetic trait
While human lifespan is only moderately heritable, “getting old” runs in families. Here, van den Berg et al. study mortality data from three-generation cohorts to define a threshold for longevity and find that individuals have an increasing survival advantage with each additional relative in the top 10% survivors of their birth cohort.
- Niels van den Berg
- , Mar Rodríguez-Girondo
- & P. Eline Slagboom
-
Article
| Open AccessTransposable elements are regulated by context-specific patterns of chromatin marks in mouse embryonic stem cells
Transposable elements (TEs) fulfill essential but poorly understood roles in genome organization and gene expression control. Here the authors show that the regulation of TEs occurs through overlapping epigenetic mechanisms that control the expression and chromatin signatures at TEs.
- Jiangping He
- , Xiuling Fu
- & Andrew P. Hutchins
-
Article
| Open AccessTrans-ethnic kidney function association study reveals putative causal genes and effects on kidney-specific disease aetiologies
Estimated glomerular filtration rate (eGFR) is a measure of kidney function used to define chronic kidney disease. Here, Morris et al. perform trans-ethnic genome-wide meta-analyses for eGFR in 312,468 individuals and identify novel loci and downstream putative causal genes.
- Andrew P. Morris
- , Thu H. Le
- & Nora Franceschini
-
Article
| Open AccessRetrotranspositional landscape of Asian rice revealed by 3000 genomes
Transposable elements (TE) are the dominant constituent of plant genomes. Here the authors develop a tool to analyze TE insertion sites in 3000 rice genomes and provide evidence for recent TE activity during cultivation and that external, rather than genetic, stimuli trigger most activations.
- Marie-Christine Carpentier
- , Ernandes Manfroi
- & Olivier Panaud
-
Article
| Open AccessGenome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins
A current challenge in genome editing is delivering Cas9 and sgRNA into target cells. Here the authors engineer a delivery system based on murine leukemia virus-like particles loaded with Cas9-sgRNA ribonucleoproteins to induce efficient genome editing in both cell culture and in vivo in mouse.
- Philippe E. Mangeot
- , Valérie Risson
- & Emiliano P. Ricci
-
Article
| Open AccessThe non-canonical SMC protein SmcHD1 antagonises TAD formation and compartmentalisation on the inactive X chromosome
The inactive X chromosome (Xi) has an atypical structure, with global loss of TADs, A/B compartments and formation of mega-domains. Here the authors show that the non-canonical SMC family protein, SmcHD1, important for developmental gene silencing on Xi, antagonises TAD formation and compartmentalization on the Xi in a transcription independent way.
- Michal R. Gdula
- , Tatyana B. Nesterova
- & Neil Brockdorff
-
Article
| Open AccessSelective single molecule sequencing and assembly of a human Y chromosome of African origin
Due to various structural and sequence complexities, the human Y chromosome is challenging to sequence and characterize. Here, the authors develop a strategy to sequence native, unamplified flow sorted Y chromosomes with a nanopore sequencing platform, and report the first assembly of a human Y chromosome of African origin.
- Lukas F. K. Kuderna
- , Esther Lizano
- & Tomas Marques-Bonet
-
Article
| Open AccessCapturing variation impact on molecular interactions in the IMEx Consortium mutations data set
Genetic variants might exert their functional effects via influencing molecular interaction. Here, the authors present a resource featuring almost 28,000 annotations describing the effect of small sequence changes on physical protein interactions, curated by IMEx Consortium curators.
- J. Khadake
- , B. Meldal
- & P. Porras
-
Article
| Open AccessEzh2 programs TFH differentiation by integrating phosphorylation-dependent activation of Bcl6 and polycomb-dependent repression of p19Arf
Ezh2 is an histone methyltransferase that catalyzes H3K27me3. Here the authors show that Ezh2 promotes T follicular helper (TFH) differentiation and helper activity, by cooperating with Tcf1 to activate Bcl6 transcription and epigenetically repressing p19Arf, an antagonist of Bcl6 function and TFH cell survival.
- Fengyin Li
- , Zhouhao Zeng
- & Hai-Hui Xue
-
Article
| Open AccessOrigin and evolution of qingke barley in Tibet
The origin of Tibetan barley (qingke) has been a controversial issue for many years. Here, the authors conduct population genomics study to support that qingke is derived from eastern domesticated barley instead of Tibetan wild barley and suggest southern Tibetan Plateau as its introduction route.
- Xingquan Zeng
- , Yu Guo
- & Nyima Tashi
-
Article
| Open AccessDistinct adaptive mechanisms drive recovery from aneuploidy caused by loss of the Ulp2 SUMO protease
Transient aneuploidy enables cells to survive sudden environmental changes before longterm cellular adaptations are established. Here, the authors show that yeast cells respond to the acute loss of Ulp2 SUMO protease by rapid induction of aneuploidy, and reveal predictable long-term adaptation mechanisms that restore euploidy.
- Hong-Yeoul Ryu
- , Francesc López-Giráldez
- & Mark Hochstrasser
-
Article
| Open AccessDissection of genetic variation and evidence for pleiotropy in male pattern baldness
Male pattern baldness (MPB) is a polygenic trait that affects the majority of European men. Here, Yap et al. estimate heritability, partitioned by autosomes and the X-chromosome, of MPB in the UK Biobank cohort, perform GWAS for MPB and find genetic correlation with other sex-specific traits.
- Chloe X. Yap
- , Julia Sidorenko
- & Peter M. Visscher
-
Article
| Open AccessGenome re-sequencing reveals the evolutionary history of peach fruit edibility
Peach is an economically important fruit crop. Here, the authors carry out a large-scale population genomics analysis of peach, describing its demographic history as well as genes associated with domestication and edibility traits.
- Yang Yu
- , Jun Fu
- & Hua Xie
-
Article
| Open AccessHigh-resolution genome-wide functional dissection of transcriptional regulatory regions and nucleotides in human
Millions of enhancers are predicted, but their validation remains challenging. Here, the authors report genome-wide enhancer function quantification and high-resolution dissection for millions of accessible DNA fragments, revealing driver nucleotides and helping interpret non-coding disease variants.
- Xinchen Wang
- , Liang He
- & Manolis Kellis
-
Article
| Open AccessLatin Americans show wide-spread Converso ancestry and imprint of local Native ancestry on physical appearance
Latin Americans trace their ancestry to the admixture of Native Americans, Europeans and Sub-Saharan Africans. Here, the authors develop a novel haplotype-based approach and analyse over 6,500 Latin Americans to infer the geographically-detailed genetic structure of this population.
- Juan-Camilo Chacón-Duque
- , Kaustubh Adhikari
- & Andrés Ruiz-Linares
-
Article
| Open AccessGenomic insights into multidrug-resistance, mating and virulence in Candida auris and related emerging species
Candida auris is an emergent fungal pathogen that is resistant to multiple antifungals. Here, Muñoz et al. analyse genomic sequences for isolates from each of the four major C. auris clades and for three related species, and identify genetic features associated with virulence, antifungal resistance and mating.
- José F. Muñoz
- , Lalitha Gade
- & Christina A. Cuomo
-
Article
| Open AccessLocal and global chromatin interactions are altered by large genomic deletions associated with human brain development
Copy number variants in the human genome (CNVs) are associated with neurodevelopmental and psychiatric disorders such as schizophrenia and autism. Here the authors investigate how the large deletion CNV on chromosome 22q11.2 alters chromatin organization.
- Xianglong Zhang
- , Ying Zhang
- & Alexander E. Urban
-
Article
| Open AccessKinase-dead ATR differs from ATR loss by limiting the dynamic exchange of ATR and RPA
ATR kinase is a key regulator of chromosome integrity. Here the authors by analysing the phenotype of a mouse model expressing a kinase-dead ATR, reveal the effect of ATR inhibition compared to ATR loss and its consequences for meiosis, DNA replication, checkpoint activation and genome instability .
- Demis Menolfi
- , Wenxia Jiang
- & Shan Zha
-
Article
| Open AccessFunctional genomic analysis identifies miRNA repertoire regulating C. elegans oocyte development
Biogenesis and function of miRNAs in somatic cells is well characterized, but their role in the germline has not been established. Here the authors identify and characterize Drosha independent and dependent miRNAs during C. elegans oogenesis, revealing their roles in meiotic progression and oocyte development.
- Amanda L. Minogue
- , Michael R. Tackett
- & Swathi Arur
-
Article
| Open AccessHuman sex reversal is caused by duplication or deletion of core enhancers upstream of SOX9
SRY and its target SOX9 are known key determinants in testis development. Here the authors by studying duplications and deletions upstream of SOX9 from patient samples with disorders of sex development (DSD) reveal enhancers for SOX9 critical for human sex development and DSD.
- Brittany Croft
- , Thomas Ohnesorg
- & Andrew Sinclair
-
Article
| Open AccessSpatial fine-mapping for gene-by-environment effects identifies risk hot spots for schizophrenia
Schizophrenia (SCZ) risk is influenced by genetic and environmental factors. Here, the authors develop a statistical method for analyzing gene-by-environment effects in SCZ risk across Denmark with fine spatial resolution.
- Chun Chieh Fan
- , John J. McGrath
- & Carsten Bøcker Pedersen
-
Article
| Open AccessKinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling
IFT-A proteins and Kinesin-2 modulate canonical Wnt/Wg-signalling independent of their ciliary role, but how is unclear. Here, the authors show that Kinesin-2 and IFT-A act as a complex to promote nuclear translocation of β-catenin in Drosophila and mouse MEF Wnt signalling independent of its ciliary role.
- Linh T. Vuong
- , Carlo Iomini
- & Marek Mlodzik
-
Article
| Open AccessVirus-mediated export of chromosomal DNA in plants
Viruses are potential vectors for horizontal gene transfer. Here, studying viral infection of sugar beet plants, the authors report the generation of virus-host circular DNA hybrids and provide a picture of the initial steps in virus-mediated horizontal transfer of chromosomal DNA between plant species.
- Marco Catoni
- , Emanuela Noris
- & Gian Paolo Accotto
-
Article
| Open AccessGenome-wide meta-analysis implicates mediators of hair follicle development and morphogenesis in risk for severe acne
Acne vulgaris is a chronic inflammation of the skin, the genetic basis of which is incompletely understood. Here, Petridis et al. perform GWAS and meta-analysis for acne in 26,722 individuals and identify 12 novel risk loci that implicate structure and maintenance of the skin in severe acne risk.
- Christos Petridis
- , Alexander A. Navarini
- & Michael A. Simpson
-
Article
| Open AccessTranscriptomic meta-signatures identified in Anopheles gambiae populations reveal previously undetected insecticide resistance mechanisms
Increasing insecticide resistance of mosquitoes represents a public health threat, and underlying mechanisms are poorly understood. Here, Ingham et al. identify putative insecticide resistance genes in Anopheles gambiae populations across Africa and develop a web-based application that maps their expression.
- V. A. Ingham
- , S. Wagstaff
- & H. Ranson
-
Article
| Open AccessGenome-wide study of hair colour in UK Biobank explains most of the SNP heritability
Natural hair colour in Europeans is a complex genetic trait. Here, the authors carry out a genome-wide association study using UK BioBank data, suggesting that in combination with pigmentation genes, variants with roles in hair texture and growth can affect hair colouration or our perception of it.
- Michael D. Morgan
- , Erola Pairo-Castineira
- & Ian J. Jackson
-
Article
| Open AccessGWAS identifies 14 loci for device-measured physical activity and sleep duration
Studying the genetic underpinnings of physical activity and sleep duration can be confounded by self-reporting. Here, Doherty et al. use data from 91,105 UK Biobank participants, whose activity had been monitored for a week by a wearable device, for genome-wide association analysis and identify 14 loci.
- Aiden Doherty
- , Karl Smith-Byrne
- & Cecilia M. Lindgren
-
Article
| Open AccessGenome-wide mega-analysis identifies 16 loci and highlights diverse biological mechanisms in the common epilepsies
Epilepsies are common brain disorders and are classified based on clinical phenotyping, imaging and genetics. Here, the authors perform genome-wide association studies for 3 broad and 7 subtypes of epilepsy and identify 16 loci - 11 novel - that are further annotated by eQTL and partitioned heritability analyses.
- Bassel Abou-Khalil
- , Pauls Auce
- & Fritz Zimprich
-
Article
| Open AccessNorthern forest tree populations are physiologically maladapted to drought
Northern tree populations may not benefit under climate change, with implications for assisted migration and range expansion. Here, Isaac-Renton et al. show that leading-edge lodgepole pine populations have fewer characteristics of drought-tolerance, so may not adapt to tolerate drier conditions.
- Miriam Isaac-Renton
- , David Montwé
- & Kerstin Treydte
-
Article
| Open AccessPan-cancer characterisation of microRNA across cancer hallmarks reveals microRNA-mediated downregulation of tumour suppressors
miRNAs have emerged as regulators of diverse biological processes including cancer. Here the authors present an extended pan-cancer analysis of the miRNAs in 15 epithelial cancers; integrating methylation, transcriptomic and mutation data they reveal alternative mechanisms of tumour suppressors’ regulation in absence of mutation, methylation or copy number alterations.
- Andrew Dhawan
- , Jacob G. Scott
- & Francesca M. Buffa
Browse broader subjects
Browse narrower subjects
- Agricultural genetics
- Animal breeding
- Behavioural genetics
- Cancer genetics
- Cancer genomics
- Clinical genetics
- Consanguinity
- CRISPR-Cas systems
- Cytogenetics
- Development
- Epigenetics
- Epigenomics
- Eukaryote
- Evolutionary biology
- Functional genomics
- Gene expression
- Gene regulation
- Genetic association study
- Genetic hybridization
- Genetic interaction
- Genetic linkage study
- Genetic markers
- Genome
- Genomic instability
- Genomics
- Genotype
- Haplotypes
- Heritable quantitative trait
- Immunogenetics
- Inbreeding
- Medical genetics
- Microbial genetics
- Mutation
- Neurodevelopmental disorders
- Plant breeding
- Plant genetics
- Polyploidy
- Population genetics
- Prokaryote
- Quantitative trait
- RNA splicing
- RNAi
- Sequencing