Gene expression analysis

Gene expression analysis is the study of gene products by imaging, amplification, probe hybridization or sequencing-based detection methods.

Latest Research and Reviews

  • Research | | open

    Transcriptome analysis of circulating tumor cells (CTCs) provides insights into monitoring target therapeutics and underlying tumor metastasis. Here the authors present Hydro-Seq, a contamination-free high-throughput hydrodynamic scRNA-seq barcoding technique for rare CTCs.

    • Yu-Heng Cheng
    • , Yu-Chih Chen
    • , Eric Lin
    • , Riley Brien
    • , Seungwon Jung
    • , Yu-Ting Chen
    • , Woncheol Lee
    • , Zhijian Hao
    • , Saswat Sahoo
    • , Hyun Min Kang
    • , Jason Cong
    • , Monika Burness
    • , Sunitha Nagrath
    • , Max S. Wicha
    •  & Euisik Yoon
  • Reviews |

    For appropriate control of gene expression, enhancers must communicate with the right target genes at the right time, typically over large genomic distances. In this Review, Schoenfelder and Fraser discuss our latest understanding of long-range enhancer–promoter crosstalk, including target-gene specificity, interaction dynamics, protein and RNA architects of interactions, roles of 3D genome organization and the pathological consequences of regulatory rewiring.

    • Stefan Schoenfelder
    •  & Peter Fraser
  • Research | | open

    Master transcription factors dominantly direct cell fate and barriers ensuring their tissue specific silencing are not clearly defined. Here, the authors demonstrate that inefficient targeted transactivation of Sox1 in neural progenitor cells is surmountable through targeted promoter demethylation using dCas9-Tet1.

    • Valentin Baumann
    • , Maximilian Wiesbeck
    • , Christopher T. Breunig
    • , Julia M. Braun
    • , Anna Köferle
    • , Jovica Ninkovic
    • , Magdalena Götz
    •  & Stefan H. Stricker
  • Research | | open

    Juan Feng et al. show that hawk tea extract reduces cholesterol by suppressing Niemann-Pick C1 like 1-mediated free cholesterol uptake and decreasing the production of very low-density lipoprotein through hepatocyte nuclear factor 4 alpha. This study suggests a therapeutic potential of hawk tea extract for hypercholesterolemia.

    • Juan Feng
    • , Jian Yang
    • , Yujun Chang
    • , Liansheng Qiao
    • , Honglei Dang
    • , Kun Luo
    • , Hongyan Guo
    • , Yannan An
    • , Chengmei Ma
    • , Hong Shao
    • , Jie Tian
    • , Yuan Yuan
    • , Lan Xie
    • , Wanli Xing
    •  & Jing Cheng
  • Research | | open

    Size and shape of bones are important for height and body shape. Here, Styrkarsdottir et al identify 12 loci in a GWAS for bone area derived from DXA scans and show that these loci associate with other bone-related phenotypes including osteoarthritis, height, bone mineral density and risk of hip fracture.

    • Unnur Styrkarsdottir
    • , Olafur A. Stefansson
    • , Kristbjorg Gunnarsdottir
    • , Gudmar Thorleifsson
    • , Sigrun H. Lund
    • , Lilja Stefansdottir
    • , Kristinn Juliusson
    • , Arna B. Agustsdottir
    • , Florian Zink
    • , Gisli H. Halldorsson
    • , Erna V. Ivarsdottir
    • , Stefania Benonisdottir
    • , Hakon Jonsson
    • , Arnaldur Gylfason
    • , Kristjan Norland
    • , Katerina Trajanoska
    • , Cindy G. Boer
    • , Lorraine Southam
    • , Jason C. S. Leung
    • , Nelson L. S. Tang
    • , Timothy C. Y. Kwok
    • , Jenny S. W. Lee
    • , Suzanne C. Ho
    • , Inger Byrjalsen
    • , Jacqueline R. Center
    • , Seung Hun Lee
    • , Jung-Min Koh
    • , L. Stefan Lohmander
    • , Lan T. Ho-Pham
    • , Tuan V. Nguyen
    • , John A. Eisman
    • , Jean Woo
    • , Ping-C. Leung
    • , John Loughlin
    • , Eleftheria Zeggini
    • , Claus Christiansen
    • , Fernando Rivadeneira
    • , Joyce van Meurs
    • , Andre G. Uitterlinden
    • , Brynjolfur Mogensen
    • , Helgi Jonsson
    • , Thorvaldur Ingvarsson
    • , Gunnar Sigurdsson
    • , Rafn Benediktsson
    • , Patrick Sulem
    • , Ingileif Jonsdottir
    • , Gisli Masson
    • , Hilma Holm
    • , Gudmundur L. Norddahl
    • , Unnur Thorsteinsdottir
    • , Daniel F. Gudbjartsson
    •  & Kari Stefansson

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