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| Open AccessRevealing chiral cell motility by 3D Riesz transform-differential interference contrast microscopy and computational kinematic analysis
The lack of an appropriate method has hampered quantitative measurements of cell chirality. Here, the authors combine Riesz transform-differential interference contrast microscopy and computational kinematic analysis to reveal chiral cell motility of neuronal growth cone filopodia and cellular slime mold.
- Atsushi Tamada
- & Michihiro Igarashi
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Article
| Open AccessDefective Gpsm2/Gαi3 signalling disrupts stereocilia development and growth cone actin dynamics in Chudley-McCullough syndrome
Mutations inGPSM2cause a rare disease characterized by deafness and brain abnormalities. Here the authors show that Gpsm2 forms a molecular complex with a heterotrimeric G-protein subunit, whirlin and a myosin motor to regulate actin dynamics in neurons and auditory hair cell stereocilia.
- Stephanie A. Mauriac
- , Yeri E. Hien
- & Mireille Montcouquiol
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| Open AccessFormin and capping protein together embrace the actin filament in a ménage à trois
Formins promote actin filament polymerization and capping protein blocks polymerization; both proteins are thought to exclude each other from barbed ends. Here the authors show that both proteins can simultaneously bind barbed ends in a ternary complex while enhancing each other's dissociation from the barbed end.
- Shashank Shekhar
- , Mikael Kerleau
- & Marie-France Carlier
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Article
| Open AccessIRSp53 senses negative membrane curvature and phase separates along membrane tubules
The inverted-BAR domain protein IRSp53 associates with the inner leaflet of tubular membranes such as filopodia. Here, Prévostet al. demonstrate that the I-BAR domain of IRSp53 senses negative membrane curvature, and undergoes phase separation which may aid its clustering upon filopodia generation.
- Coline Prévost
- , Hongxia Zhao
- & Patricia Bassereau
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| Open AccessA Rac/Cdc42 exchange factor complex promotes formation of lateral filopodia and blood vessel lumen morphogenesis
Blood vessel development depends upon endothelial cell migration and adhesion, which are regulated by Rho-GTPases. Here the authors identify Rho-GTPase guanine nucleotide exchange factors that specifically control lateral filopodial contacts and are required for lumen formation during angiogenesis.
- Sabu Abraham
- , Margherita Scarcia
- & Georgia Mavria
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| Open AccessThe structure of FMNL2–Cdc42 yields insights into the mechanism of lamellipodia and filopodia formation
FMNL formins polymerize actin filaments to generate cellular protrusions such as lamellipodia and filopodia at the leading edge of a cell. Here the authors provide detailed mechanistic insights into the formation of actin-based protrusions through GTPase dependent activation and membrane localization of FMNL1 and FMNL2.
- Sonja Kühn
- , Constanze Erdmann
- & Matthias Geyer