Coat complexes

  • Article
    | Open Access

    Cytosolic coat proteins capture secretory cargo and sculpt membrane carriers for intracellular transport, such as COPII which mediates Endoplasmic Reticulum to Golgi trafficking of thousands of cargoes. Here authors visualise the complete, membrane-assembled COPII coat by cryo-electron tomography and subtomogram averaging, revealing the full network of interactions within and between coat layers.

    • Joshua Hutchings
    • , Viktoriya G. Stancheva
    •  & Giulia Zanetti
  • Article
    | Open Access

    The COPII coat assembles in two concentric layers and mediates protein export from the endoplasmic reticulum. Here the authors present the 4.9 Å resolution cryo-tomography and subtomogram averaging structure of the membrane bound COPII inner coat that was obtained by in vitro reconstitution and discuss mechanistic implications.

    • Joshua Hutchings
    • , Viktoriya Stancheva
    •  & Giulia Zanetti
  • Article
    | Open Access

    The transport of secretory proteins from the endoplasmic reticulum to the Golgi depends on COPII-coated vesicles. Here, the authors show that activation-induced alternative splicing of Sec16 controls adaptation of COPII transport to increased secretory cargo upon T cell activation.

    • Ilka Wilhelmi
    • , Regina Kanski
    •  & Florian Heyd