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| Open AccessBiomimetic α-selective ribosylation enables two-step modular synthesis of biologically important ADP-ribosylated peptides
ADP-ribosylated peptides are important molecular tools, however, the lack of effective synthetic methods hinders the access to their complex structures. Here, the authors present a biomimetic α-selective ribosylation reaction coupled with click chemistry ultimately providing a two-step modular synthesis of α-ADP-ribosylated peptides.
- Anlian Zhu
- , Xin Li
- & Lingjun Li
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Article
| Open AccessLight-induced primary amines and o-nitrobenzyl alcohols cyclization as a versatile photoclick reaction for modular conjugation
Developing new click chemistry reactions for robust molecular assembly remains challenging. Here the authors report a light-induced primary amines and o-nitrobenzyl alcohols photoclick cyclization for rapid and modular functionalization of small molecules and native biomolecules, in vitro and in living systems.
- An-Di Guo
- , Dan Wei
- & Xiao-Hua Chen
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Article
| Open AccessCRISPRoff enables spatio-temporal control of CRISPR editing
Uncontrolled gene editing can lead to off-target effects and chromosomal translocations. Here the authors develop CRISPRoff, light degradable sgRNAs for titratable and spatially defined gene editing.
- Jared Carlson-Stevermer
- , Reed Kelso
- & Travis Maures
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Article
| Open AccessFunctional genetic encoding of sulfotyrosine in mammalian cells
Protein tyrosine O-sulfation is crucial for biomolecular interactions. Here the authors report in vitro engineering and in vivo validation of a tyrosyl-tRNA synthetase mutant for the genetic encoding of sulfotyrosine in mammalian cells.
- Xinyuan He
- , Yan Chen
- & Wei Niu
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Article
| Open AccessStructural bases of IMiD selectivity that emerges by 5-hydroxythalidomide
5-hydroxythalidomide is a primary thalidomide metabolite generated by the cytochrome P450 isozymes. The reported data, including crystal structure of the 5-hydroxythalidomide-mediated complex of CRBN with SALL4, elucidate how additional hydroxy group of the metabolite enhances the interaction of CRBN with the neosubstrate SALL4.
- Hirotake Furihata
- , Satoshi Yamanaka
- & Takuya Miyakawa
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Article
| Open AccessExploiting natural chemical photosensitivity of anhydrotetracycline and tetracycline for dynamic and setpoint chemo-optogenetic control
Anhydrotetracycline and tetracycline are commonly used chemicals to regulate transcription and translation, respectively. Here the authors exploit the natural photosensitivity of these molecules to place their activity under optical control.
- Armin Baumschlager
- , Marc Rullan
- & Mustafa Khammash
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Article
| Open AccessChimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion
Orthogonal aminoacyl-tRNA synthetase/tRNA pairs are crucial for the incorporation of unnatural amino acids in a site-specific manner. Here the authors use rational chimera design to create multiple efficient pairs that function in bacterial and mammalian systems for genetic code expansion.
- Wenlong Ding
- , Hongxia Zhao
- & Shixian Lin
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Article
| Open AccessA cysteine selenosulfide redox switch for protein chemical synthesis
Control of cysteine reactivity is of paramount importance for the synthesis of proteins using native chemical ligation. Here, the authors report a readily cleavable N-selenoethyl group attached to cysteine and apply it to the modular assembly of linear and cyclic polypeptides.
- Vincent Diemer
- , Nathalie Ollivier
- & Oleg Melnyk
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Article
| Open AccessChemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing
Chemical modification of proteins can be used to decipher function or use that function for therapeutic purposes. Here, the authors insert synthetic peptides via tandem protein trans-splicing to add post-translational modifications or non-canonical amino acids.
- K. K. Khoo
- , I. Galleano
- & S. A. Pless
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Article
| Open AccessHigh-resolution snapshots of human N-myristoyltransferase in action illuminate a mechanism promoting N-terminal Lys and Gly myristoylation
N-terminal glycine myristoyl transferases (NMTs) catalyse the myristoylation of eukaryotic proteins. Here, the authors provide insights into the catalytic mechanism of NMTs by determining the crystal structures of human NMT1 in complex with reactive cognate lipid and peptide substrates and further show that NMT1 also catalyses the acylation of N-terminal lysines.
- Cyril Dian
- , Inmaculada Pérez-Dorado
- & Carmela Giglione
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Article
| Open AccessTargeting the tumor vasculature with engineered cystine-knot miniproteins
Cystine-knot miniprotein are small, highly stable, disulfide-rich peptides with increasing potential as drugs and tumor imaging agents. Here the authors develop cystine-knot miniproteins targeting the vascular tumor marker EDB, and use them as probes for in vivo tumor vasculature imaging.
- Bonny Gaby Lui
- , Nadja Salomon
- & Ugur Sahin
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Article
| Open AccessNAIL-MS reveals the repair of 2-methylthiocytidine by AlkB in E. coli
Bacterial tRNA is modified by thiolation of nucleosides. Here the authors identify 2-methylthiocytidine in bacterial tRNA using nucleic acid isotope labeling coupled mass spectrometry. Exposure to methylating agents converts 2-thiocytidine to 2-methylthiocytidine, which is repaired by demethylase AlkB in vivo.
- Valentin F. Reichle
- , Dimitar P. Petrov
- & Stefanie Kellner
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Article
| Open AccessPhosphorylated lipid-conjugated oligonucleotide selectively anchors on cell membranes with high alkaline phosphatase expression
Membrane-anchored DNA probes have been used to study molecular interactions and control cell assembly, but are not selective for different cell membranes. Here the authors develop a lipid-conjugated oligonucleotide for alkaline phosphatase-dependent cell membrane anchorage and use it to distinguish different cancer cells.
- Cheng Jin
- , Jiaxuan He
- & Weihong Tan
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Article
| Open AccessSingle-site glycine-specific labeling of proteins
Single-site labelling of proteins is desirable, e.g., for analytical purposes. Here, the authors developed a method in which they use an aldol-type reaction to modify proteins at N-terminal glycine residues in an efficient and selective manner, which is also applicable to cell lysates.
- Landa Purushottam
- , Srinivasa Rao Adusumalli
- & Vishal Rai
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Article
| Open AccessBacterial glycosyltransferase-mediated cell-surface chemoenzymatic glycan modification
Glycan molecules can be modified directly on the cell surface via chemoenzymatic approaches. Here, the authors employ a set of four bacterial glycosyltransferases to develop a live cell-based killing assay to probe host cell glycan-mediated influenza A virus infection.
- Senlian Hong
- , Yujie Shi
- & Peng Wu
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Article
| Open AccessDinitroimidazoles as bifunctional bioconjugation reagents for protein functionalization and peptide macrocyclization
The selective formation of protein bioconjugates under physiological conditions is a challenging task. Here, the authors report that 1,4-dinitroimidazoles are reagents of choice for protein bioconjugation at either cysteine or lysine sites within short times and provide facile access to peptide macrocycles.
- Qunfeng Luo
- , Youqi Tao
- & Huan Wang
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Article
| Open AccessStructural insights into trans-histone regulation of H3K4 methylation by unique histone H4 binding of MLL3/4
MLL3 and MLL4 are members of the SET1/MLL family of histone H3K4 methyltransferases, which are responsible for monomethylating histone H3K4 on enhancers. Here the authors show that an extended PHD domain (ePHD6) in MLL3 and MLL4 specifically recognizes an H4H18-containing fragment of histone H4, and that modifications of residues surrounding H4H18 modulate H4 binding to MLL3/4.
- Yanli Liu
- , Su Qin
- & Jinrong Min
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Article
| Open AccessTranslation of non-standard codon nucleotides reveals minimal requirements for codon-anticodon interactions
The recognition of the mRNA codon by the tRNA anticodon is crucial for protein synthesis. Here the authors introduce non-standard nucleotides in bacterial and eukaryotic mRNA to reveal the minimal hydrogen bond requirement of codon-anticodon interaction for efficient and accurate translation.
- Thomas Philipp Hoernes
- , Klaus Faserl
- & Matthias David Erlacher
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Article
| Open AccessStructural insights into the π-π-π stacking mechanism and DNA-binding activity of the YEATS domain
YEATS domains are histone acylation readers that recognize crotonyllysine and acetyllysine. Here the authors provide structural insights into how YEATS domains recognize acetyllysines and further show that the human AF9 YEATS domain also binds DNA.
- Brianna J. Klein
- , Kendra R. Vann
- & Tatiana G. Kutateladze
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Article
| Open AccessAsymmetric assembly of high-value α-functionalized organic acids using a biocatalytic chiral-group-resetting process
Alpha-functionalized organic acids are building blocks of many bioactive compounds. Here, the authors developed a toolbox-like, modular set of enzymes that reset chiral groups, turning achiral glycine and simple aldehydes into stereodefined α-keto acids, α-hydroxy acids, and α-amino acids.
- Wei Song
- , Jin-Hui Wang
- & Li-Ming Liu
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Article
| Open AccessManipulating and visualizing the dynamic aggregation-induced emission within a confined quartz nanopore
The difficulty in recovering the aggregation-induced emission fluorogens (AIEgens) to the initial dispersed state upon illuminating has limited their applications. Here, the authors employ the confined space in the quartz nanopore to achieve a nanopore-size dependent restriction of AIEgens.
- Yi-Lun Ying
- , Yuan-Jie Li
- & He Tian
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Article
| Open AccessSelective N-terminal acylation of peptides and proteins with a Gly-His tag sequence
His-tagged proteins can undergo N-terminal acylation as an undesired side-reaction. Here, the authors utilize this to develop a method for highly selective acylation and further modification of peptides and proteins using an optimized His sequence and 4-methoxyphenyl esters as acyl donors.
- Manuel C. Martos-Maldonado
- , Christian T. Hjuler
- & Knud J. Jensen
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Article
| Open AccessThe identification of carbon dioxide mediated protein post-translational modifications
Carbon dioxide can interact with proteins to form carbamate post-translational modifications. Here, the authors developed a strategy to identify carbamate post-translational modifications by trapping carbon dioxide and subsequently identifying the carbamylated proteins.
- Victoria L. Linthwaite
- , Joanna M. Janus
- & Martin J. Cann
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Article
| Open AccessWater-dispersible PEG-curcumin/amine-functionalized covalent organic framework nanocomposites as smart carriers for in vivo drug delivery
Despite their potential application as drug-delivery carriers, covalent organic frameworks (COF) have been only evaluated in vitro. Here the authors show by real time tracking in vivo the cell uptake of anticancer-drug loaded and water dispersible COFs.
- Guiyang Zhang
- , Xinle Li
- & Xudong Jia
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Article
| Open AccessRapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide
Chemically modifying proteins is hard to achieve selectively without purifying the target protein. Here, the authors present a method to modify proteins on lysine residues in living cells quicker than via known approaches and show that it can be used to develop protein covalent inhibitors.
- Tomonori Tamura
- , Tsuyoshi Ueda
- & Itaru Hamachi
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Article
| Open AccessReversible glycosidic switch for secure delivery of molecular nanocargos
Retention of drugs loaded into liposomes is a major challenge to effective targeted drug delivery. Here, the authors report on the modification of drugs with a glycosidic pH sensitive switch to improve encapsulation and retention of drugs and demonstrate application in an in vivo cancer model.
- Pierre-Alain Burnouf
- , Yu-Lin Leu
- & Steve R. Roffler
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Article
| Open AccessCombinatorial hydrogels with biochemical gradients for screening 3D cellular microenvironments
Tools to investigate a wide range of 3D microenvironmental parameters are important for understanding and controlling cell fate. Here, the authors develop hydrogels with orthogonal biochemical gradients and use this screening system to identify microenvironments that induce mesenchymal stem cell chondrogenesis.
- Sebastián L. Vega
- , Mi Y. Kwon
- & Jason A. Burdick
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Article
| Open AccessCreation of a long-acting nanoformulated dolutegravir
Current ART for treatment of HIV-1 infection requires a strict daily regimen adherence. Herein, the authors report the manufacture and characterization of a nanoformulated dolutegravir prodrug with improved cell and tissue penetration, a remarkable apparent half-life and the potential for bimonthly drug administration.
- Brady Sillman
- , Aditya N. Bade
- & Howard E. Gendelman
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Article
| Open AccessStructural basis for IL-1α recognition by a modified DNA aptamer that specifically inhibits IL-1α signaling
The cytokine interleukin 1α (IL-1α) plays an important role in inflammatory processes. Here the authors use SELEX to generate a modified DNA aptamer which specifically binds IL-1α, present the structure of the IL-1α/aptamer complex and show that this aptamer inhibits the IL-1α signaling pathway.
- Xiaoming Ren
- , Amy D. Gelinas
- & Anna Marie Pyle
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Article
| Open AccessChemical labelling for visualizing native AMPA receptors in live neurons
Visualizing neurotransmitter receptor traffic is fundamental to understanding brain functions, but the current imaging methods can compromise the process. Here, the authors synthesize small fluorescent probes to label endogenous glutamate receptors in cell cultures and brain tissues, without affecting their function.
- Sho Wakayama
- , Shigeki Kiyonaka
- & Itaru Hamachi
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Article
| Open AccessA brain-sparing diphtheria toxin for chemical genetic ablation of peripheral cell lineages
Diphtheria toxin selectively kills cells engineered to express the diphtheria toxin receptor (DTR). Here the authors report a PEGylated version of diphtheria toxin that does not enter the brain, allowing for ablation of only peripheral cells when using Cre lines that drive DTR expression in both the periphery and in the brain.
- Mafalda M. A. Pereira
- , Inês Mahú
- & Ana I. Domingos
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Article
| Open AccessStoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents
Current cysteine bioconjugation strategies for protein-drug conjugates synthesis often yield heterogeneous and poorly stable products. Here, the authors use carbonylacrylic derivatives to selectively modify cysteine residues and synthesize biologically functional antibody conjugates highly stable in plasma.
- Barbara Bernardim
- , Pedro M.S.D. Cal
- & Gonçalo J. L. Bernardes
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Article
| Open AccessAbundant DNA 6mA methylation during early embryogenesis of zebrafish and pig
DNA 6mA is a poorly understood epigenetic mark present at a low abundance in eukaryotic genomes. Here the authors observe high levels in zebrafish and pig during early embryogenesis enriched to repetitive regions of the genome and followed by attenuation during development.
- Jianzhao Liu
- , Yuanxiang Zhu
- & Chuan He
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Article
| Open AccessAromatic thiol-mediated cleavage of N–O bonds enables chemical ubiquitylation of folded proteins
Chemical approaches to site-specifically ubiquitylate a target protein allow investigation of the biochemical effects of this modification, but they often destabilize the protein. Here, the authors report on a synthetic conjugation strategy that leads to protein ubiquitylation in non-denaturing conditions.
- Caroline E. Weller
- , Abhinav Dhall
- & Champak Chatterjee
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Article
| Open AccessUnexpected functional implication of a stable succinimide in the structural stability of Methanocaldococcus jannaschii glutaminase
Succinimide is a post-translational modification susceptible to rapid hydrolysis and generally associated with protein destabilisation. Here, the authors use mass spectroscopy to identify a stable succinimide intermediate that is responsible for the high thermostability of a thermophilic enzyme.
- Sanjeev Kumar
- , Sunita Prakash
- & Hemalatha Balaram
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Article
| Open AccessChemoselective synthesis and analysis of naturally occurring phosphorylated cysteine peptides
Protein phosphorylation mediates signalling and other important cellular processes, but the specific effect of cysteine phosphorylation is unclear. Here, the authors present a chemical strategy to selectively phosphorylate cysteine residues and a mass spectrometry approach to detect and characterize endogenous pCys sites.
- Jordi Bertran-Vicente
- , Martin Penkert
- & Christian P. R. Hackenberger
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Article
| Open AccessStructural basis for oxygen degradation domain selectivity of the HIF prolyl hydroxylases
The response to hypoxia involves multiple genes regulated by the hypoxia inducible transcription factors (HIFs), whose stability is regulated by prolyl hydroxylation. Here the authors provide a molecular basis for the substrate selectivity of the HIF prolyl hydroxylases that can be altered in erythrocytosis and cancer.
- Rasheduzzaman Chowdhury
- , Ivanhoe K. H. Leung
- & Christopher J. Schofield
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Article
| Open AccessProtein S-sulfenylation is a fleeting molecular switch that regulates non-enzymatic oxidative folding
Protein S-sulfenylation is a posttranslational modification that can act as a sensor of redox oxidative stress. Here the authors show that, following mechanical unfolding, sulfenic acid drives disulfide bond reformation and guides non-enzymatic oxidative folding.
- Amy E. M. Beedle
- , Steven Lynham
- & Sergi Garcia-Manyes
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Article
| Open AccessPeptide–oligonucleotide conjugates as nanoscale building blocks for assembly of an artificial three-helix protein mimic
Peptide and oligonucleotide systems are known to self-assemble both in nature and artificial systems. Here, the authors combine both forms of self-assembly through the synthesis of peptideoligonucleotide conjugates and show formation of a three-helix structure that dimerises at higher concentrations.
- Chenguang Lou
- , Manuel C. Martos-Maldonado
- & Knud J. Jensen
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Article
| Open AccessChemical basis for the recognition of trimethyllysine by epigenetic reader proteins
A structurally diverse set of epigenetic reader proteins can recognize methylated lysine residues on histones. Here the authors show that recognition of trimethyllysine occurs through a combination of favourable cation–πinteractions and the release of water molecules occupying the aromatic cages of reader proteins.
- Jos J.A.G. Kamps
- , Jiaxin Huang
- & Jasmin Mecinović
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Article
| Open AccessSupramolecular amplification of amyloid self-assembly by iodination
Amyloid assemblies lie at the heart of many physiological functions, as well as being the cause of numerous diseases. Here, the authors subtly modify wild-type pentapeptides with halides, and discover that the new halogen bonding interactions have a remarkable influence on their physical properties.
- Arianna Bertolani
- , Lisa Pirrie
- & Pierangelo Metrangolo
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Article |
AAA+ chaperones and acyldepsipeptides activate the ClpP protease via conformational control
Acyldepsipeptides are natural antibiotics that function by activating the ClpP protease and deregulating proteolysis. Here, Gersch et al.show that acyldepsipeptides not only increase access to the active sites but also exert conformational control, thereby allosterically stimulating ClpP catalysis.
- Malte Gersch
- , Kirsten Famulla
- & Stephan A. Sieber
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Review Article |
Selective chemical protein modification
The chemical modification of proteins is an important tool for probing natural systems and synthesizing novel conjugates. Here, Spicer and Davis review the merits and limitations of the most useful methods for selective modification at both natural and unnatural amino acids.
- Christopher D. Spicer
- & Benjamin G. Davis
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Article
| Open AccessEvolutionarily conserved intracellular gate of voltage-dependent sodium channels
The location of the activation gate in voltage-gated sodium channels is not clear. Here, the authors report that a conserved intracellular gate consisting of a ring of hydrophobic residues regulates access to the pore.
- Kevin Oelstrom
- , Marcel P. Goldschen-Ohm
- & Baron Chanda
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Article |
2′-OMe-phosphorodithioate-modified siRNAs show increased loading into the RISC complex and enhanced anti-tumour activity
Short interfering siRNAs—siRNAs—have therapeutic potential in the treatment of disease; however, their delivery to target tissues is difficult. Here, Wu et al. chemically modify siRNAs and show that this improves loading into the siRNA silencing machinery and thus efficacy in eliminating cancer cells in mice.
- Sherry Y. Wu
- , Xianbin Yang
- & Anil K. Sood
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Article
| Open Access4′-O-substitutions determine selectivity of aminoglycoside antibiotics
Aminoglycoside antibiotics target the ribosome but their limited selectivity for the bacterial ribosome can cause side effects in humans. Here, the authors synthesize 4′-O-ether or 4′,6′-O-acetal modifications and show that these compounds possess increased selectivity against bacterial ribosomes.
- Déborah Perez-Fernandez
- , Dmitri Shcherbakov
- & Erik C. Böttger
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Article
| Open AccessComparative cross-linking and mass spectrometry of an intact F-type ATPase suggest a role for phosphorylation
Rotary ATPases are membrane-embedded motors that produce or consume ATP and control pH within cells. Schmidt et al.use mass spectrometry to characterize the intact chloroplast ATPase from spinach and, using comparative cross-linking, show that phosphorylation affects stability and nucleotide occupancy.
- Carla Schmidt
- , Min Zhou
- & Carol V. Robinson
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Article
| Open AccessArginine clustering on calix[4]arene macrocycles for improved cell penetration and DNA delivery
Arginine-rich peptides act as delivery systems for the internalization of cargoes in cells. Here, the clustering of arginine units in a parallel array on a macrocyclic scaffold produces a vector with high efficiency in DNA delivery and transfection.
- Valentina Bagnacani
- , Valentina Franceschi
- & Rocco Ungaro
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Article |
CaV1.3-selective L-type calcium channel antagonists as potential new therapeutics for Parkinson's disease
L-type calcium channels comprising the CaV1.3 subunit have been linked to the generation of mitochondrial oxidant stress in Parkinson’s disease. Kang et al. identify pyrimidine-2,4,6-triones as a potential molecular scaffold, which they modify to develop a potent and highly selective CaV1.3 antagonist.
- Soosung Kang
- , Garry Cooper
- & Richard B. Silverman