Featured
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Basal stem cell progeny establish their apical surface in a junctional niche during turnover of an adult barrier epithelium
Galenza et al. show that basal stem cell progeny seamlessly integrate into a physiologically active barrier epithelium by gestating their future apical surface in a sheltering niche created by a transient occluding junction.
- Anthony Galenza
- , Paola Moreno-Roman
- & Lucy Erin O’Brien
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Cell fate coordinates mechano-osmotic forces in intestinal crypt formation
Yang, Xue et al. demonstrate in intestinal organoids that region-specific cell fates drive actomyosin patterns and modulate luminal osmotic forces to coordinate morphogenesis.
- Qiutan Yang
- , Shi-Lei Xue
- & Prisca Liberali
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Article |
FERARI is required for Rab11-dependent endocytic recycling
Solinger et al. show that FERARI is a conserved tethering platform that mediates Rab11-dependent recycling at sorting endosomes.
- Jachen A. Solinger
- , Harun-Or Rashid
- & Anne Spang
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News & Views |
Endfoot regrowth for neural stem cell renewal
Tangential expansion of neural stem cells in the mammalian neocortex increases the number of cortical columns. A new study shows that neural stem cells that become detached from the apical surface during division regenerate an apical endfoot to ensure tangential expansion in the early stage but later lose this ability when radial expansion occurs.
- Masafumi Tsuboi
- & Yukiko Gotoh
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Article |
Endfoot regeneration restricts radial glial state and prevents translocation into the outer subventricular zone in early mammalian brain development
Fujita et al. show that endfoot regeneration of radial glia cells after division retains them in the ventricular zone in early development, independently of spindle orientation, but is lost during late neurogenesis.
- Ikumi Fujita
- , Atsunori Shitamukai
- & Fumio Matsuzaki
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Review Article |
Mesenchymal–epithelial transition in development and reprogramming
This Review discusses the mesenchymal–epithelial transition and its roles in development, cellular fate conversions and somatic cell reprogramming.
- Duanqing Pei
- , Xiaodong Shu
- & Jean Paul Thiery
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Class III phosphatidylinositol-3-OH kinase controls epithelial integrity through endosomal LKB1 regulation
O’Farrell et al. show that class III PI3K regulates epithelial integrity through endosomal LKB1. Class III PI3K inactivation dysregulates LKB1 to alter cell polarity, and the PtdIns3P effector WDFY2 regulates LKB1.
- Fergal O’Farrell
- , Viola Hélène Lobert
- & Tor Erik Rusten
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Article |
EGFR signalling controls cellular fate and pancreatic organogenesis by regulating apicobasal polarity
In the developing mouse pancreas, EGFR regulates apical polarity via PI(3)K and Rac1 and elicits different ligand-dependent effects: BTC enables β-cell commitment and EGF inhibits polarization of epithelial progenitors during the primary transition.
- Zarah M. Löf-Öhlin
- , Pia Nyeng
- & Henrik Semb
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Developmental regulation of apical endocytosis controls epithelial patterning in vertebrate tubular organs
Bagnat, Martín-Belmonte and colleagues reveal that plasmolipin (PLLP) is upregulated in the zebrafish midgut during development and controls epithelial patterning by promoting polarized endocytosis.
- Alejo E. Rodríguez-Fraticelli
- , Jennifer Bagwell
- & Fernando Martín-Belmonte
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Rap2A links intestinal cell polarity to brush border formation
Microvilli are essential for the function of intestinal cells. Bos and colleagues have found that the polarity kinase LKB-1 induces PtdIns(4,5)P2 enrichment at the apical membrane. This leads to the successive accumulation of phosphatidic acid and the small GTPase Rap2A with its GEF and its effectors. These, in turn, trigger the changes in the actin cytoskeleton responsible for microvilli formation.
- Martijn Gloerich
- , Jean Paul ten Klooster
- & Johannes L. Bos
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Caenorhabditis elegans screen reveals role of PAR-5 in RAB-11-recycling endosome positioning and apicobasal cell polarity
RAB-11-positive recycling endosomes participate in the establishment and maintenance of epithelial polarity. Zerial and colleagues carry out an in vivo image-based RNAi screen for factors that regulate recycling endosome positioning in Caenorhabditis elegans. They identify, among other candidates, PAR-5 as a key determinant of recycling endosome positioning and, thus, apicobasal polarity.
- Julia Franziska Winter
- , Sebastian Höpfner
- & Marino Zerial
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News & Views |
Making sense of glycosphingolipids in epithelial polarity
A potential role for glycosphingolipids and lipid rafts in apical sorting was initially met with enthusiasm, but genetic analysis has since provided little support for it. A report now establishes that glycosphingolipids mediate apical sorting, and specifically help maintain apicobasal polarity in Caenorhabditis elegans.
- Vincent Hyenne
- & Michel Labouesse
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Article |
Apicobasal domain identities of expanding tubular membranes depend on glycosphingolipid biosynthesis
Cell polarity is critically important for organogenesis. Using a series of RNA-interference-based screens, Göbel and colleagues reveal the role of the glycosphingolipid glucosylceramide (GlcCer) in determining apicobasal polarity and maintaining the organization of the intestinal lumen in the developing worm.
- Hongjie Zhang
- , Nessy Abraham
- & Verena Göbel
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Letter |
Vangl2 directs the posterior tilting and asymmetric localization of motile primary cilia
Several recent studies have proposed that the planar cell polarity pathway (PCP) regulates cilia formation. Zebrafish embryos lacking the core PCP component Vangl2 do not have defects in ciliogenesis, but instead show impairment of primary cilia position and orientation.
- Antonia Borovina
- , Simone Superina
- & Brian Ciruna