Structural and functional analyses reveal that ATG9 is a lipid scramblase that transports phospholipids between the inner and outer leaflets in liposomes and promotes autophagosome membrane expansion.

See Articles by Noda and Otomo.

Aggregation of human islet amyloid polypeptide (IAPP, or amylin) is linked to type 2 diabetes. Cryo-EM analyses of fibrils formed by IAPP, wild-type or S20G variant, reveal the bases for aggregation and polymorphism.

See Article by Gallardo et al.

A cryo-EM structure of Y. lipolitica mitochondrial complex I reveals ordered water molecules involved in proton relay and catalysis.

See Article by Grba and Hirst

Two nanobodies that recognize the receptor-binding domain of SARS-CoV-2 spike glycoprotein block its interaction with receptor ACE2, thus neutralizing the virus.

See Article by Huo et al.

DPPA2 and DPPA4 maintain bivalency at developmental gene promoters and protect them from DNA methylation, keeping them poised for future lineage-specific activation.

See article Reik and article Hackett

Cryo-EM structures and electrophysiology analyses of the cGMP-activated CNG channel TAX-4 provide insights into channel gating and regulation.

See Zheng et al.

Yeast studies and mathematical modeling reveal that the size of the amyloid seed influences transitions between prion states and [PSI+] persistence.

See Villali et al.

smFRET analysis of Cas1–Cas2 shows that transcription promotes efficient and directional CRISPR spacer integration.

See Budhathoki et al.

Circular nanoparticles that self-assemble from designed tandem repeat proteins with different copy numbers and at defined positions are functionalized with cargoes.

See Correnti et al.

thCHART, a hybridization capture approach using biotinylated LNA-containing oligonucleotides with toehold architecture, improves the specificity of RNA enrichment and enables detection of lncRNA chromosomal spreading patterns.

See Machyna et al.

Global neddylation profiling identifies conjugation sites in non-cullin substrates. Neddylation of cofilin regulates, via actin dynamics, dendrite length and complexity in developing neurons.

See Vogl et al.

Deep mutational scanning data for multiple kinases reveals common residues that mediate drug resistance across the mammalian kinome.

See Persky et al.