PrimeViews

This PrimeView highlights the underlying mechanisms of C–H activation and the utility of this synthetic manifold across applications such as polymers synthesis and drug discovery.

This PrimeView highlights how electrochemical impedance spectroscopy is used to study the mechanisms of electrochemical reactions across different applications.

This PrimeView highlights the key steps for reconstructing a super-resolution image from single-molecule localization data.

This PrimeView highlights how computational approaches can be used to characterise medium-sized molecular systems investigated using different spectroscopic techniques, focusing on the role of computation to help understand spectroscopic phenomena and how accuracy and interpretability are coupled.

This PrimeView highlights the use of scanning probe microscopy across a range of applications, from chemistry and materials science to studying living systems.

This PrimeView highlights how electron paramagnetic resonance spectroscopy can be used to detect and understand the evolution of dynamic species with unpaired electrons during various catalytic cycles, focusing on current and future experimental setups for active catalyst detection and the diverse applications this in situ technique enables.

This PrimeView highlights the different techniques used for acquiring information on the spatial organization of proteins within the cell.

This PrimeView describes the broad set of chemistries available within the bioorthogonal chemistry toolbox and how these have been used across a broad set of applications.

This PrimeView highlights various parallelization techniques to concurrently acquire different NMR signals, and focuses on how increases in data acquisition speeds and sensitivity lead to quicker elucidation of molecular structures.

This PrimeView highlights the different setups for optical tweezers and their applications, with a focus on the various force measurements they enable on single molecules such as DNA and molecular motors.

This PrimeView highlights the most relevant aspects of information theory that facilitate automation and prediction for chemical synthesis. This summary provides an overview of the software, hardware and data and how these are used to enable retrosynthetic logic, reaction prediction and automation.

This PrimeView describes the SORS instrument, discusses data analysis methods and highlights some of the many applications of SORS.

This PrimeView highlights the main considerations for the use of CLIP methods and shows the key experimental steps common to most CLIP techniques.

This PrimeView highlights the cross-disciplinary nature of X-ray computed tomography, with a focus on important experimental aspects such as 3D data acquisition and 3D data reconstruction.

This PrimeView highlights important experimental and analytical aspects of conducting ancient DNA analysis, with a focus on collaboration and sample preservation.

This PrimeView highlights the design, assembly and assessment of 2D and 3D nanostructures made using DNA origami.

This PrimeView highlights the main chromatin accessibility profiling methods. These methods include DNase-seq, ATAC-seq, MNase-seq, and DNA methylation-based methods to assess open chromatin and regulatory elements.

This PrimeView highlights the process of producing 2D materials using chemical vapour deposition. Advances in this technique have increased yield and quality, and are being used for industrial applications.

This PrimeView highlights recombination-mediated genome engineering methods common in microbial synthetic biology. The use of one technique in particular, MAGE, is discussed in terms of its optimization and use in biotechnology and to improve human health.

Bayesian statistics are an approach to data analysis based on Bayes’ Theorem. This PrimeView provides an overview of how to select and establish priors, likelihoods, and posteriors. The use of Bayesian statistics and modelling across disciplines is discussed, along with appropriate sharing of data acquired using Bayesian methods.