Volume 15 Issue 10, October 2014

Volume 15 Issue 10

'3D culture' by Vicky Summersby, inspired by the Review on p647.

Research Highlights


  • Review Article |

    Mitochondria contain a genome that is inherited maternally; this complicates their segregation during cell division, oogenesis and development. Mechanisms that ensure mitochondrial integrity include fusion and fission processes, organelle transport, mitophagy and genetic selection. Defects in these processes can lead to cell and tissue pathologies.

    • Prashant Mishra
    •  & David C. Chan
  • Review Article |

    Recent advances in three-dimensional (3D) culture techniques have increased our understanding of the cellular mechanisms that drive epithelial tissue development, the genetic regulation of cell behaviours in epithelial tissues and the role of microenvironmental factors in normal development and disease. 3D culture can be used to build complex organs and to advance therapeutic approaches.

    • Eliah R. Shamir
    •  & Andrew J. Ewald
  • Review Article |

    Recent studies suggest that mechanisms of de novo lumen formation in different systems — such as the zebrafish vasculature, C. elegans excretory cells, the D. melanogaster trachea and three-dimensional cultures of endothelial and MDCK cells — share some common features. They all involve expansion of the apical plasma membrane, vesicle transport and regulation of the microtubule and actin cytoskeletons.

    • Sara Sigurbjörnsdóttir
    • , Renjith Mathew
    •  & Maria Leptin
  • Review Article |

    The actin capping activity of capping protein (CP) is indirectly regulated by competing with other factors for filament binding, or directly by factors that bind CP and sterically inhibit its interactions with filaments. Other proteins interact with CP through their 'capping protein interaction' (CPI) motif and modulate its activity via allosteric effects.

    • Marc Edwards
    • , Adam Zwolak
    • , Dorothy A. Schafer
    • , David Sept
    • , Roberto Dominguez
    •  & John A. Cooper



  • Essay |

    To achieve effective visual communication of complex data, it is important that biologists identify the needs of their audience — whether they are peers or an outreach audience. This guide to the available wealth of resources, ranging from software tools to professional illustrators, should help researchers to generate better figures and presentations tailored to the needs of any audience.

    • Graham T. Johnson
    •  & Samuel Hertig