News & Comment

In this Tools of the Trade article, Nouf Laqtom (at the Abu-Remaileh lab) describes the LysoIP system used to tag and isolate lysosomes for multi-omic approaches and to determine their composition under physiological and pathological states.

Quality control of misfolded glycosylphosphatidylinositol-anchored proteins (GPI-APs) does not occur through ER-associated degradation, as the lipid anchor precludes retrotranslocation across the ER membrane. Instead, different organisms have developed distinct solutions to prevent the accumulation of misfolded GPI-APs.

A new study collaboratively delivered by Valentina Greco’s and Smita Krishnaswamy’s labs provides new insight into patterns of calcium signalling in epithelial tissues using intravital imaging of the basal layer of the mouse epidermis.

Zuzana Storchová recounts the discovery of the subtle yet significant consequences of polyploidy.

The eukaryotic genome is organized into topologically associating domains by cohesin and CTCF. Davidson et al. now reveal a role for DNA tension in the regulation of the CTCF–cohesin interaction to modulate domain boundaries.

Numerous databases collate data on long non-coding RNAs (lncRNAs); Ma and Zhang describe their range and discuss their contribution to lncRNA research.

The discovery that peripheral organs such as the spleen support blood formation following radiation has inspired haematology research for over five decades.

In this Tools of the Trade article, Benjamin Jackson (at the Finley lab) describes the use of genetic co-essentiality analysis to interrogate the assembly of metabolic networks, fuelling discovery of new aspects of metabolism.

Cold temperature prolongs lifespan in nematodes by inducing ubiquitin-independent proteasome activity, which prevents protein aggregation and neuronal degeneration. The pathway is conserved in humans.

Shelby et al. demonstrate the existence of discrete domains in plasma membranes of B cells that selectively partition membrane-associated proteins.

Thoru Pederson recalls the impact that a 1968 paper from Dan Brown’s lab had on his career.

McCarthy et al. identify distinct populations of smooth muscle cells in the intestine that support the establishment of the intestinal stem cell niche during postnatal development by supplying trophic signals to enable niche expansion.

Self-assembly of replication protein A (RPA) into dynamic condensates after binding of single-stranded DNA promotes telomere maintenance in cancer cells.

Stable introns were serendipitously discovered just over a decade ago.

The lab of C. David Allis was among the pioneers of developing histone-PTM-specific antibodies to unlock the mysteries of chromatin.

Weems et al. show that sustained membrane blebbing promotes pro-survival signalling in extracellular matrix-detached cells.

Scott et al. describe a mechanism based on C-degron mimicry by which an E3 ubiquitin ligase discriminates bona fide substrates from non-physiological substrates.

In this Tools of the Trade article, Laura Capolupo (at D’Angelo lab) describes the approach to study cell-to-cell heterogeneity in lipid content and its correspondence to cell identity.

Tyrosine sulfation is a novel histone post-translational modification, which facilitates transcription.

In this Tools of the Trade article, Patrik Risteski (at Tolić lab) describes high-throughput fluorescent speckle microscopy (FSM) based on a cell-permeable dye that allows to study dynamics of individual microtubules in human cells.