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Transcription activator-like effector nucleases (TALENs) comprise a nonspecific nuclease fused to a sequence-specific DNA-binding domain. This domain can be engineered so that TALENs can target virtually any sequence. TALENs are an efficient tool to modify genes in a wide range of cell types and organisms.
Cryo-electron tomography has provided a means of characterizing the architecture of macromolecular complexes in their native environment, and facilitated a better understanding of cellular processes. By combining this method with fluorescence and super-resolution microscopy, the full potential of this approach can be realized.
