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A preprint by Nakandakari-Higa et al. introduces universal LIPSTIC (uLIPSTIC), a labelling technique for the detection of physical interactions between any cell types without the requirement for specific receptor–ligand interaction.
In this Tools of the Trade article, Christa Haase (at the Charles Lin lab) describes a technique called Image-seq that enables image-guided cell isolation from a specific, intact tissue location for subsequent single-cell gene expression analysis.
A preprint by Sbierski-Kind et al. shows that IL-33-producing adventitial fibroblasts provide a stromal niche for ILC2s that limits IL-17-mediated liver fibrosis.
In this Tools of the Trade article, Xiaoyun Dai (of the Sidi Chen lab) developed a CRISPR-based platform termed CLASH to generate a large pool of precisely gene-modified CAR T cells, from which the most desirable variants could be selected.
Gillian Griffiths and colleagues show that T cell receptor downregulation at the immune synapse occurs through ectocytosis, which terminates receptor signalling and promotes dissociation from target cells.
A combination therapy of a tumour-targeted antibody–cytokine fusion protein and a chemotherapy drug shows promising results in preclinical testing and in a phase I study in patients with recurrent glioblastoma.
A preprint by Lad et al. shows that tumour-associated neutrophils in glioblastoma originate from skull bone marrow and acquire an antigen-presenting cell phenotype intratumorally in the presence of local T cells.
Copper(ii) ions promote the metabolic and epigenetic remodelling of macrophages towards a pro-inflammatory state. A copper-binding metformin dimer showed promising results in mouse models of inflammation.
A preprint by Mahadevan et al. shows that pancreatitis alters the immune landscape of mice with pancreatic ductal adenocarcinoma and highlights a role for dendritic cells in promoting responses to immune checkpoint blockade.