Two groups have developed a technique for analysing the transcriptome-wide distribution of the RNA modification N6-methyladenosine (m6A) that incorporates antibody-mediated enrichment of the modification coupled with sequencing. Notably, in humans and mice, there was an enrichment for the mark around stop codons. In addition, conservation of modification sites between these species indicates some functionality for this mark, as does its tissue-specific distribution in human RNAs.
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03 July 2012
The title of one of the papers referenced in this article contained an important typographical error. The correct title is 'Comprehensive analysis of mRNA methylation reveals enrichment in 3′ UTRs and near stop codons'.
ORIGINAL RESEARCH PAPERS
Meyer, K. D. et al. Comprehensive analysis of mRNA methylation reveals enrichment in 3′ UTRs and near stop codons. Cell 17 May 2012 (doi:10.1016/j.cell.2012.05.003)
Dominissini, D. et al. Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq. Nature 485, 201–205 (2012)
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Stower, H. Transcriptome-wide N6-methyladenosine analysis. Nat Rev Genet 13, 452 (2012). https://doi.org/10.1038/nrg3280
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DOI: https://doi.org/10.1038/nrg3280
