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By quantifying over 100 Cannabis samples for terpene and cannabinoid content and genotyping them for over 100,000 single nucleotide polymorphisms, this study finds that Cannabis labelling is associated with genetic variants in terpene synthase genes.
Field photographs are essential for research and conservation, but are difficult to access since there is no central database or connection between archives. The 25 leading databases hold only 53% of the plant species of the Americas, illustrating the need for increased work on collecting and standardizing this valuable data.
A prime-editing-library-mediated saturation mutagenesis method is developed and used to substantially expand the herbicide-resistant OsACC1 mutations, showing an advantage over base-editing-mediated mutagenesis in the in planta screening of functional mutations.
Not only is climate change having an impact on invasive plants through changes in ecosystem, atmospheric carbon may be changing the plants themselves. This analysis finds higher concentrations of parthenin at higher levels of CO2, making an invasive weed more toxic.
Despite the perception that plant science focuses on strictly scientific criteria, this analysis finds that there is an aesthetic bias in regards to which plants, based on certain traits, receive more research attention.
Species once considered extinct have now, through improved taxonomic knowledge and collection activities, been restored, with lessons for conservation policies both in Europe and globally.
The authors obtain a cryogenic electron microscopy structure of the cryptochrome blue-light receptor CRY2 in a light-induced tetramer complex. The key residues involved in oligomerization are characterized and validated by mutational analysis.
Genomic analyses of 101 plant species reveals a fundamental shift in the proportion of repetitive sequences in genomes above around 10 Gbp—species with the largest genomes are only about 55% repetitive, and this proportion does not increase further with genome size.
A new multicolour tool named PlaCCI allows for visualization and quantification of discrete cell cycle phases thanks to the sequential expression of three specific fluorescent markers.
This study developed a user-friendly genome editing system that can efficiently conditionally knock out target genes in a temporally inducible and cell-type-specific manner.
Potato roots can be infected by parasitic nematodes that cause galls. Here the authors express a plant peptide in soil bacteria that stimulates plant immunity. Pre-treatment of potato roots with the modified bacteria protects the plants from nematodes.
An efficient and multiplexed in planta gene editing approach is developed by infecting Cas9 transgenic plants with an RNA virus that expresses single guide RNAs carrying sequences that confer cell-to-cell mobility.
The highly efficient and specific Cas12b is a new tool for genome editing with CRISPR in rice. This system can also be engineered for targeted transcriptional repression and activation.
The widely used SpCas9-NG tool can edit genomic targets, but unfortunately also the guide RNA sequences that are present in the transfer DNA and integrated into the plant genome. The authors propose a strategy to alleviate this self-editing risk.
Arabidopsis lateral root radial emergence is spatially constrained by the dual xylem poles in the main root. However, the asymmetric presence of water can modify the orientation of lateral roots at a very early stage.
Mepiquat chloride is a growth retardant used in cotton farming to optimize growth by inhibiting gibberellin metabolism, but it is not effective in other crops. Now, its molecular target is found, enabling selective agrochemical inhibition of growth in cereals.
Faster onset of photoprotection could potentially increase biomass accumulation. However, Arabidopsis lines overexpressing three photoprotective proteins had impaired growth rate under fluctuating light, despite rapidly triggering photoprotection.
Broomrape (Orobanche) is a parasitic plant that infects crops. Here, quantitative genetics and mapping in sunflower identify one resistance gene named HaOr7. It is a membrane receptor-like kinase that prevents attachment of the parasitic plant to the sunflower roots.