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Huang et al. develop a potent genome editing toolkit to generate transgene-free genome-edited plants in the T0 generation by co-editing of ALS gene (without obvious fitness costs) and gene(s) of interest via Agrobacterium-mediated transient expression.
This study introduces a new multiplexed fluorescence in situ hybridization method, PHYTOMap, that enables single-cell and spatial analysis of gene expression in whole-mount plant tissue in a transgene-free manner and at low cost.
Strigolactone (SL) regulates plant development. Structural analysis shows that when SL binds its receptor ShHTL5, conformational changes occur including the creation of internal tunnels that posit an explanation for how SL by-products exit the receptor after signalling.
In plants, mechanically triggered signalling cascades involve intracellular calcium waves. The kinetics of waves induced by the application and release of force to a cell are distinct, suggesting that plants distinguish touch from letting go.
Callixylon wood from the Late Devonian (approximately 360 Myr ago) is found to contain tyloses, swellings of parenchyma cells into adjacent water-conducting cells that develop in response to embolism and pathogen infection.
The integration of single-nucleus and spatial transcriptomics is used to profile dynamic changes during soybean nodule maturation. The approach identifies a rare cell type that specifically expresses numerous genes required for symbiotic nitrogen fixation.
This study identifies two genes that are important for protecting wheat against blast fungus, a pathogen that also infects related grasses. This work helps identify potential strategies for breeding wheat with improved resistance to this damaging fungus.
On the basis of 38,994 records of giant trees belonging to 237 species across Japan, this study shows that macroecological processes such as climate may drive spiritual ecosystem services obtained from giant trees.
The lack of haploid male fertility is a bottleneck to improve the efficiency in doubled haploid technology for accelerating crop breeding. This study shows that mutations in the parallel spindle genes are sufficient to restore haploid male fertility in Arabidopsis.