Volume 4 Issue 3, March 2019

Volume 4 Issue 3

Algal metabolic mapping goes viral

Combining the plaque assay with mass spectrometry imaging allowed metabolites produced during viral infection of the alga Emiliania huxleyi to be mapped at high spatiotemporal resolution, and identified a shift in lipid metabolism.

See Schleyer et al.

Image: Guy Schleyer and Yonghui Dong. Cover Design: Samantha Whitham.

Editorial

  • Editorial |

    When accurate and thoughtfully presented, reporting of good science in the popular press should be celebrated and encouraged by researchers. In return, tabloid headline writers should dial down their hyperbolic rhetoric and avoid sensationalism when reporting scientific discoveries.

News & Views

  • News & Views |

    Molecular players involved in systemic and acute infections are relatively easy to pinpoint, whereas bacterial resilience during chronic infections remains less well understood. Pseudomonas aeruginosa encodes a quorum-regulated virulence factor, TesG, that promotes chronic lung infection by suppressing host inflammatory responses.

    • Alain Filloux
    •  & Jane C. Davies
  • News & Views |

    Klebsiella pneumoniae isolated from patients with primary sclerosing cholangitis was found to cause intestinal barrier dysfunction resulting in T-helper-17-cell-mediated hepatobiliary injury, providing evidence for specific gut-derived, pore-forming pathogens as triggers for immune-mediated liver disease.

    • Peter Fickert
    •  & Hanns-Ulrich Marschall
  • News & Views |

    Bacteria have previously been assumed to cope with environmental stress by tuning their total number of active ribosomes. Instead, a study in this issue of Nature Microbiology shows that from a heterogeneous pool of ribosomes, Vibrio vulnificus uses ribosomes with a particular ribosomal RNA variant to translate upregulated stress response mRNAs.

    • Kathrin Leppek
    •  & Maria Barna
  • News & Views |

    Severe fever with thrombocytopenia virus is an emerging, highly lethal tick-borne pathogen with growing impact. In this issue of Nature Microbiology, two papers make major progress towards a better understanding of its so far incompletely understood mechanisms of virulence.

    • Jennifer Deborah Wuerth
    •  & Friedemann Weber

Research

  • Brief Communication |

    Adaptive laboratory evolution experiments in Escherichia coli show that the pseudogene efeU can be repaired to restore the bacterium’s iron uptake system, demonstrating that pseudogenes may serve as an ‘adaptive repertoire’ of selectable traits.

    • Amitesh Anand
    • , Connor A. Olson
    • , Laurence Yang
    • , Anand V. Sastry
    • , Edward Catoiu
    • , Kumari Sonal Choudhary
    • , Patrick V. Phaneuf
    • , Troy E. Sandberg
    • , Sibei Xu
    • , Ying Hefner
    • , Richard Szubin
    • , Adam M. Feist
    •  & Bernhard O. Palsson
  • Letter |

    Měnglà virus (MLAV) is a phylogenetically distinct bat filovirus, whose genome shares 32–54% nucleotide sequence identity with known filoviruses. MLAV glycoprotein-typed pseudo-types can transduce cell lines derived from humans, monkeys, dogs, hamsters and bats.

    • Xing-Lou Yang
    • , Chee Wah Tan
    • , Danielle E. Anderson
    • , Ren-Di Jiang
    • , Bei Li
    • , Wei Zhang
    • , Yan Zhu
    • , Xiao Fang Lim
    • , Peng Zhou
    • , Xiang-Ling Liu
    • , Wuxiang Guan
    • , Libiao Zhang
    • , Shi-Yue Li
    • , Yun-Zhi Zhang
    • , Lin-Fa Wang
    •  & Zheng-Li Shi
  • Letter |

    A bacterial strain that requires the neurotransmitter GABA for growth was identified and used to isolate GABA-producing bacteria, including Bacteroides spp., from human stool samples; the relative abundance of Bacteroides was negatively correlated with an altered GABA-mediated response in a depression patient cohort.

    • Philip Strandwitz
    • , Ki Hyun Kim
    • , Darya Terekhova
    • , Joanne K. Liu
    • , Anukriti Sharma
    • , Jennifer Levering
    • , Daniel McDonald
    • , David Dietrich
    • , Timothy R. Ramadhar
    • , Asama Lekbua
    • , Nader Mroue
    • , Conor Liston
    • , Eric J. Stewart
    • , Marc J. Dubin
    • , Karsten Zengler
    • , Rob Knight
    • , Jack A. Gilbert
    • , Jon Clardy
    •  & Kim Lewis
  • Letter |

    Genome sequencing of fractionated T-, B- and natural killer cells from patients with chronic active Epstein–Barr virus (EBV) infection sheds light on the nature of the EBV-infected progenitor and suggests a link between intragenic EBV deletions and EBV-associated neoplastic proliferations.

    • Yusuke Okuno
    • , Takayuki Murata
    • , Yoshitaka Sato
    • , Hideki Muramatsu
    • , Yoshinori Ito
    • , Takahiro Watanabe
    • , Tatsuya Okuno
    • , Norihiro Murakami
    • , Kenichi Yoshida
    • , Akihisa Sawada
    • , Masami Inoue
    • , Keisei Kawa
    • , Masao Seto
    • , Koichi Ohshima
    • , Yuichi Shiraishi
    • , Kenichi Chiba
    • , Hiroko Tanaka
    • , Satoru Miyano
    • , Yohei Narita
    • , Masahiro Yoshida
    • , Fumi Goshima
    • , Jun-ichi Kawada
    • , Tetsuya Nishida
    • , Hitoshi Kiyoi
    • , Seiichi Kato
    • , Shigeo Nakamura
    • , Satoko Morishima
    • , Tetsushi Yoshikawa
    • , Shigeyoshi Fujiwara
    • , Norio Shimizu
    • , Yasushi Isobe
    • , Masaaki Noguchi
    • , Atsushi Kikuta
    • , Keiji Iwatsuki
    • , Yoshiyuki Takahashi
    • , Seiji Kojima
    • , Seishi Ogawa
    •  & Hiroshi Kimura
  • Letter |

    The structure of enterovirus 71 in complex with its receptor SCARB2 provides insights into the mechanism of viral uncoating within the endo/lysosome compartment and identifies few conserved key residues within the binding footprint that might facilitate the design of receptor mimic therapeutics.

    • Daming Zhou
    • , Yuguang Zhao
    • , Abhay Kotecha
    • , Elizabeth E. Fry
    • , James T. Kelly
    • , Xiangxi Wang
    • , Zihe Rao
    • , David J. Rowlands
    • , Jingshan Ren
    •  & David I. Stuart
  • Letter |

    The non-structural protein NSs of the severe fever with thrombocytopenia syndrome virus interacts with ABIN2 and promotes formation and signalling of the TPL2–ABIN2–p105 kinase complex to induce expression of the immune-suppressive cytokine IL-10 and enhance viral pathogenesis.

    • Younho Choi
    • , Su-Jin Park
    • , Yinyan Sun
    • , Ji-Seung Yoo
    • , Raghavendra Sumanth Pudupakam
    • , Suan-Sin Foo
    • , Woo-Jin Shin
    • , Sally B. Chen
    • , Philip N. Tsichlis
    • , Won-Ja Lee
    • , Jong-Soo Lee
    • , Wenhui Li
    • , Benjamin Brennan
    • , Young-Ki Choi
    •  & Jae U. Jung
  • Article |

    An age-dependent immunocompetent ferret model for severe fever with thrombocytopenia syndrome phlebovirus (SFTSV) infection and pathogenesis recapitulates the clinical manifestations of human infections, including severe thrombocytopenia, reduced white blood cell counts and high fever with 93% mortality rate.

    • Su-Jin Park
    • , Young-Il Kim
    • , Angela Park
    • , Hyeok-Il Kwon
    • , Eun-Ha Kim
    • , Young-Jae Si
    • , Min-Suk Song
    • , Chul-Ho Lee
    • , Kyle Jung
    • , Woo-Jin Shin
    • , Jianxiong Zeng
    • , Younho Choi
    • , Jae U. Jung
    •  & Young Ki Choi
  • Article |

    Antimicrobial peptide resistance genes are found to be widespread in the gut microbiome but are exchanged at lower rates compared to antibiotic resistance genes, with functional compatibility between bacteria being important for gene exchange.

    • Bálint Kintses
    • , Orsolya Méhi
    • , Eszter Ari
    • , Mónika Számel
    • , Ádám Györkei
    • , Pramod K. Jangir
    • , István Nagy
    • , Ferenc Pál
    • , Gergely Fekete
    • , Roland Tengölics
    • , Ákos Nyerges
    • , István Likó
    • , Anita Bálint
    • , Tamás Molnár
    • , Balázs Bálint
    • , Bálint Márk Vásárhelyi
    • , Misshelle Bustamante
    • , Balázs Papp
    •  & Csaba Pál

    Collection:

  • Article |

    A type I secretion effector, TesG, suppresses host immune response to promote chronic Pseudomonas aeruginosa infection.

    • Kelei Zhao
    • , Wujiao Li
    • , Jing Li
    • , Teng Ma
    • , Kailun Wang
    • , Yang Yuan
    • , Jing Shirley Li
    • , Rou Xie
    • , Ting Huang
    • , Yige Zhang
    • , Yingshun Zhou
    • , Nongyu Huang
    • , Wenling Wu
    • , Zhen Wang
    • , Jun Zhang
    • , Bisong Yue
    • , Zongguang Zhou
    • , Jiong Li
    • , Yu-Quan Wei
    • , Xiuyue Zhang
    •  & Xikun Zhou
  • Article |

    Integration of longitudinal gut metagenomic datasets from children in Finland, Estonia and Russian Karelia reveals high strain-level diversity, which consequently impacts the functional capabilities of the early life microbiome.

    • Tommi Vatanen
    • , Damian R. Plichta
    • , Juhi Somani
    • , Philipp C. Münch
    • , Timothy D. Arthur
    • , Andrew Brantley Hall
    • , Sabine Rudolf
    • , Edward J. Oakeley
    • , Xiaobo Ke
    • , Rachel A. Young
    • , Henry J. Haiser
    • , Raivo Kolde
    • , Moran Yassour
    • , Kristiina Luopajärvi
    • , Heli Siljander
    • , Suvi M. Virtanen
    • , Jorma Ilonen
    • , Raivo Uibo
    • , Vallo Tillmann
    • , Sergei Mokurov
    • , Natalya Dorshakova
    • , Jeffrey A. Porter
    • , Alice C. McHardy
    • , Harri Lähdesmäki
    • , Hera Vlamakis
    • , Curtis Huttenhower
    • , Mikael Knip
    •  & Ramnik J. Xavier
  • Article |

    By combining imaging and single-cell RNA sequencing, the authors identify genes regulated during cell growth and division and further analyse the heterogeneity of gene expression during adaptive and acute responses to changing environments.

    • Malika Saint
    • , François Bertaux
    • , Wenhao Tang
    • , Xi-Ming Sun
    • , Laurence Game
    • , Anna Köferle
    • , Jürg Bähler
    • , Vahid Shahrezaei
    •  & Samuel Marguerat
  • Article |

    Klebsiella pneumoniae from the gut microbiota of patients with primary sclerosing cholangitis (PSC) can damage the intestinal epithelial barrier, resulting in bacterial translocation and T helper 17 cell responses in the liver, indicating a role in PSC pathogenesis.

    • Nobuhiro Nakamoto
    • , Nobuo Sasaki
    • , Ryo Aoki
    • , Kentaro Miyamoto
    • , Wataru Suda
    • , Toshiaki Teratani
    • , Takahiro Suzuki
    • , Yuzo Koda
    • , Po-Sung Chu
    • , Nobuhito Taniki
    • , Akihiro Yamaguchi
    • , Mitsuhiro Kanamori
    • , Nobuhiko Kamada
    • , Masahira Hattori
    • , Hiroshi Ashida
    • , Michiie Sakamoto
    • , Koji Atarashi
    • , Seiko Narushima
    • , Akihiko Yoshimura
    • , Kenya Honda
    • , Toshiro Sato
    •  & Takanori Kanai

    Collection:

  • Article |

    In Vibrio vulnificus, ribosomes containing variable rRNAs encoded by the rrnI operon (I-ribosomes) preferentially translate a subset of mRNAs involved in bacterial adaptation of environmental changes, establishing divergent rRNAs as regulators of gene expression.

    • Wooseok Song
    • , Minju Joo
    • , Ji-Hyun Yeom
    • , Eunkyoung Shin
    • , Minho Lee
    • , Hyung-Kyoon Choi
    • , Jihwan Hwang
    • , Yong-In Kim
    • , Ramin Seo
    • , J. Eugene Lee
    • , Christopher J. Moore
    • , Yong-Hak Kim
    • , Seong-il Eyun
    • , Yoonsoo Hahn
    • , Jeehyeon Bae
    •  & Kangseok Lee
  • Article |

    Combining the plaque assay with mass spectrometry imaging allowed high spatiotemporal resolution mapping of metabolites produced during viral infection of the alga Emiliania huxleyi, and revealed a shift in lipid metabolism towards odd-chain fatty acid lipids.

    • Guy Schleyer
    • , Nir Shahaf
    • , Carmit Ziv
    • , Yonghui Dong
    • , Roy A. Meoded
    • , Eric J. N. Helfrich
    • , Daniella Schatz
    • , Shilo Rosenwasser
    • , Ilana Rogachev
    • , Asaph Aharoni
    • , Jörn Piel
    •  & Assaf Vardi

Amendments & Corrections

Search

Quick links