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Bright reversibly switching red fluorescent proteins (rsFusionReds) with fast switching kinetics and low fatigue enable RESOLFT and MoNaLISA nanoscopy of live cells with green-orange illumination, which further reduces the risk of phototoxicity.
Ligand-inducible connection (LInC) combines HCV protease and protease inhibitors to offer an orthogonal chemogenetic approach for controlling protein functions including protein localization, gene expression, and cell–cell communication.
Stabilizable polypeptide linkages (StaPLs) based on hepatitis C virus protease enable robust, reversible, and orthogonal chemogenetic control of protein functions, including CRISPR–Cas9 activity, transcription, and protein dimerization.
Reanalysis of DNA-immunoprecipitation-based data shows that modification-specific antibodies bind unmodified short tandem repeats, and IgG controls are needed to avoid false positives.
The GeNets web platform can identify the most informative network, as well as execute, store and share network-based analyses of RNA-seq or genomic datasets.
This Analysis compares and contrasts methods for measuring the mechanical properties of cells by applying the different approaches to the same breast cancer cell line.
A direct comparison of 5′-end RNA-seq methods reveals strong performance by CAGE, and identifies differential transcriptional start site usage among brain-related samples.
Off-targets identified by whole-genome sequencing of CRISPR-treated mouse embryos and their genetic parents are compared to predictions from GUIDE-seq and computational methods.
An automated method coupling tandem mass spectrometry with high-resolution mass spectrometry imaging simultaneously reveals both the molecular structure of lipids and their spatial locations in tissue.
After CRISPR-mediated targeting of the peroxidase APEX2 to a genomic locus of interest, the proteins at that locus are labeled, enriched and identified via quantitative proteomics.
A miniature light-field microscope in combination with a signal-extraction approach enables high-speed volumetric calcium imaging in freely moving mice.
BoxCar, a mass spectrometry data acquisition method, greatly increases sensitivity and the detection of low-abundance peptides with a minimal amount of instrument time.