Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
SuReSim software simulates single-molecule localization microscopy data for structures with known ground truth models to facilitate proper design, interpretation and validation of super-resolution imaging experiments.
The SWAp-Tag method allows for easy replacement of a tag in a parental strain with an expression cassette of choice to rapidly create new yeast libraries that will enable researchers to address biological questions in Saccharomyces cerevisiae.
A high-resolution, Orbitrap-based, native mass spectrometry approach allows the direct characterization of lipid, peptide and drug binding to intact membrane proteins.
The HPN-DREAM community challenge assessed the ability of computational methods to infer causal molecular networks, focusing specifically on the task of inferring causal protein signaling networks in cancer cell lines.
This comparison of ten computational methods for detecting transcription factor binding sites in DNase hypersensitive regions in the genome determines which methods work consistently well, how DNase-seq experimental artifacts should be corrected for and which score is best for ranking methods.
This report describes an imaging and analysis platform enabling high-throughput single-molecule fluorescence investigations of fast, transient molecular recognition events.
HomoFRET sensors based on G6PD homodimerization allow direct sensing of NADPH/NADP+ redox state in living cells. Spectrally tuning the Apollo-NADP+ sensor allows multiplex imaging for studies of oxidative stress in beta cells.
Frame-projected independent-fiber photometry (FIP) enables the concurrent monitoring and manipulation of neural activity at multiple sites in the brains of freely behaving mice.
EpiGBS (epi-genotyping by sequencing) constructs de novo references using reduced representation bisulfite sequencing followed by variant calling and methylation detection.
High-field fMRI with single-vessel resolution allows one to decipher the contribution of different types of vessels to hemodynamic activity evoked by sensory or optogenetic stimulation in the rat brain.
Linking attacks can identify individuals on the basis of seemingly independent data, such as molecular phenotypes and genotypes, in different databases and are a threat to privacy. The authors statistically quantify the extent of this risk and propose means to reduce it.
Software able to assemble long synthetic reads from barcoded Illumina short-read technology improves the assembly of metagenomic data sets and analysis of the human genome.
Spectrally resolved FLIM with three excitation wavelengths and detection on 32 channels combined with advanced pattern matching allows for simultaneous detection and discrimination of fluorophores with nearly identical emission spectra, enabling highly multiplexed imaging.
PLAYR (proximity ligation assay for RNA) enables highly multiplexed transcript quantification in combination with protein marker detection in single cells using flow or mass cytometry.
A near-infrared light–activated genetically encoded photosensitizer composed of a fluorogen-activating protein and a heavy-atom–substituted fluorogen allows protein inactivation and cellular photoablation with high spatiotemporal precision and low collateral damage.
GenomeSpace is an open-source, cloud-based interoperability platform that facilitates integrative genomic analyses, allowing users to transition seamlessly between a diverse and growing set of bioinformatics tools and data resources.
By testing potential sources of biological signal that drive population-level variation in single-cell gene expression, the PAGODA software enables cells to be characterized on the basis of multiple functionally relevant features such as cell type, signaling state and cell cycle state.
A computational approach for precisely identifying and characterizing the molecular stoichiometry of multi-proteoform complexes using native top-down mass spectrometry data is reported.
Parallel, genome-wide methylation and transcriptional profiling from a single cell (scM&T-seq) enables the discovery of associations between transcriptional and epigenetic variation.
The free EMOPEC web tool enables precise tuning of bacterial protein expression on the basis of small changes to the Shine-Dalgarno sequence; oligos can be generated to engineer expression of any Escherichia coli gene.