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Growth inhibition metrics allow for robust measurement of drug efficacy independent of variables such as cell growth rate, seeding density, and growth medium; they are a practical alternative to metrics such as IC50 and offer enhanced reproducibility.
Treating glycoproteins with household bleach releases N- and O-glycans for further structural probing. Bleach treatment of glycosphingolipids releases glycan nitriles.
Conventional metrics for assessing antiproliferative drug effect have time-dependent biases that may skew results. The drug-induced proliferation rate can be used as an alternative metric for accurate and reproducible assessment of drug performance.
A method and accompanying software tool enables automated modeling of large macromolecular complexes using experimental crosslinking mass spectrometry data as distance restraints, as demonstrated for the 17-subunit yeast RNA polymerase III complex.
Single-image fluorescence lifetime imaging microscopy (siFLIM) uses a modulated-FLIM camera to record lifetimes in a single snapshot, allowing for photon-efficient and quantitative lifetime imaging of rapid cellular processes.
Microfluidic reprogramming of human somatic cells to induced pluripotent stem cells is rapid and highly efficient, enabling large-scale derivation from patient cells as well as seamless on-chip differentiation without cell expansion.
Improved sample preparation methods allow super-resolution imaging by expansion microscopy using endogenous fluorescent protein signal and conventional fluorescently labeled antibodies.
Principal component analysis on a subset of differentially methylated regions in a mixture of cell types is the basis of ReFACTor, a program to account for heterogeneity in epigenome-wide association studies (EWAS).
A method (and resource) demonstrating the mining of information from large-scale phosphoproteomics data sets is presented, allowing users to build targeted parallel reaction monitoring mass spectrometry assays to study phosphosites of interest.
Quantitative points accumulation in nanoscale topography (qPAINT) makes use of predictable binding kinetics between DNA-PAINT imager and docking strands to achieve accurate and precise counting of molecules in spatially unresolved complexes.
Enhanced CLIP yields complex libraries of RNA components of ribonucleoprotein complexes and maintains single-nucleotide resolution of binding sites. eCLIP enables large scale profiling, as demonstrated with the binding profiles of 73 RBPs in two human cancer cell lines.
Ligand-triggered ribosomal frameshifting allows control of the relative stoichiometry of two proteins and enables the building of logic gates from a single mRNA.
PanPhlAn detects strains and characterizes strain-specific gene content and activity within metagenomic and metatranscriptomic samples for microbial population analysis and epidemiology.
A saposin protein–lipid nanoparticle system stabilizes diverse, fragile membrane proteins in a lipid environment for structural and functional studies.
Lattice light-sheet and PAINT microscopy are combined to achieve low-background detection of dense molecular labels, yielding super-resolution localization microscopy images of intricate 3D structures within dividing cells and embryos.
The TraCeR tool extracts full-length, paired T cell receptor sequences from single-cell RNA-sequencing data from T lymphocytes, enabling a combination of clonotype and functional analysis.
This resource contains 394 human cell type– and tissue-specific transcriptional networks and finds that disease-associated genetic variants often perturb regulatory modules in tissues specific for that disease.