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On the cover: Wired and wireless multielectrode neurophysiological recordings in freely moving rhesus monkeys. Cover design by Katie Zhuang. Article p670
The Protein Structure Initiative will end next year; the aftershocks of this ending should be minimized for the benefit of the broader biology research community.
Two groups report the derivation of human pluripotent stem cell lines from embryos derived by somatic cell nuclear transfer using adult cells as donors.
Cell lines are better. Mice are better. Beyond disagreement about model systems and even passionate discord at times, new strategies help to explore the middle ground so models might better approximate human biology.
Adaptive optics microscopy using a de-scanned, laser-induced guide star and direct wavefront sensing allows high numerical aperture diffraction-limited imaging of fine dynamic structures deep in the intact living zebrafish brain.
Light-activated reversible inhibition by assembled trap (LARIAT) is a versatile optogenetic method for inactivating proteins, including GFP fusions, in living cells by conditional clustering at high spatiotemporal resolution.
Noise models based on the identification of major sources of technical variability in single-cell RNA-seq data allow the inference of true biological variability in cellular expression.
A graphical processing unit implementation of an efficient Bayesian-based multiview deconvolution method brings the resolution and contrast advantages of multiview deconvolution to more users of light-sheet fluorescence microscopy.
Graphene is in many ways an ideal sample support for cryo-electron microscopy, but its hydrophobicity prevents adsorption of protein from aqueous solution. Low-energy hydrogen-plasma treatment renders graphene hydrophilic and enables controlled adsorption of protein to its surface.
A two-step error correction process for high throughput–sequenced T- and B-cell receptors allows the elimination of most errors while not diminishing the natural complexity of the repertoires.
Raven calculates assembly plans for complex genetic constructs from thousands of parts. It integrates user feedback on failed intermediate assemblies to improve the final outcome.
Bone marrow formed in a cylindrical PDMS device implanted in a mouse can be surgically removed and cultured for a week in vitro without losing any of the hallmarks of in vivo bone marrow niches.
Experimental and analytical methods are described for in vivo single-molecule imaging of GFP-tagged proteins at the cell surface and are applied to the developing C. elegans embryo.
The high-throughput sequencing–RNA affinity profiling (HiTS-RAP) assay enables large-scale profiling of protein interactions with RNA libraries using a simple protocol on a high-throughput sequencer.
This framework for multiscale signal representation allows global analysis of genomic data at different length scales from base pairs to entire chromosomes and reveals the interplay of information encoded at different scales, such as the regulation of gene expression by methylation patterns that go beyond the single-gene scale.