This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Super-resolution multicolor fluorescence microscopy enabled by an apochromatic super-oscillatory lens with extended depth-of-focus
Nature Communications Open Access 22 August 2023
-
Multicolor strategies for investigating clonal expansion and tissue plasticity
Cellular and Molecular Life Sciences Open Access 20 February 2022
-
Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination
Nature Communications Open Access 20 May 2021
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Truong, T.V., Supatto, W., Koos, D.S., Choi, J.M. & Fraser, S.E. Nat. Methods 8, 757–760 (2011).
Mahou, P. et al. Nat. Methods 9, 815–818 (2012).
Andresen, V. et al. Curr. Opin. Biotechnol. 20, 54–62 (2009).
Mertz, J. Nat. Methods 8, 811–819 (2011).
Liebling, M., Forouhar, A.S., Gharib, M., Fraser, S.E. & Dickinson, M.E. J. Biomed. Opt. 10, 054001 (2005).
Acknowledgements
We thank A. McMahon and A. Stathopoulos (Caltech) for the mcd8-GFP/H2A-RFP Drosophila line, J.M. Sintes and S. Roth for technical assistance, and T.V. Truong for discussions. We thank M. Liebling and the University of California at Santa Barbara for providing Cardiac Image-Sequence Synchronization Software. This work was supported by the French Agence Nationale de la Recherche (ANR-2010-JCJC-1510-01, ANR-09-PIRI-0013, ANR-11-EQPX-0029), European Union Seventh Framework Programme (Marie Curie International Reintegration Grant no. 268379), Fondation Louis D de l'Institut de France, Human Frontier Science Program and INSERM.
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Competing interests
Work mentioned in this article is the subject of a patent application filed by the Centre National de la Recherche Scientifique, Paris, France, and the Ecole Polytechnique, Palaiseau, France, from P.M., E.B. and W.S. (WO/2013/113861).
Supplementary information
Supplementary Text, Figures and Methods
Supplementary Figures 1–5, Supplementary Tables 1 and 2 and Supplementary Methods (PDF 1501 kb)
Supplementary Video 1
Live 4D and multicolour 2P-SPIM imaging of the beating zebrafish heart (85 fps) 1.2mb (MOV 1212 kb)
Supplementary Video 2
3D cell segmentation and tracking within the entire myocardium of a beating zebrafish heart using multicolour 2P-SPIM imaging data (MOV 6912 kb)
Rights and permissions
About this article
Cite this article
Mahou, P., Vermot, J., Beaurepaire, E. et al. Multicolor two-photon light-sheet microscopy. Nat Methods 11, 600–601 (2014). https://doi.org/10.1038/nmeth.2963
Published:
Issue Date:
DOI: https://doi.org/10.1038/nmeth.2963
This article is cited by
-
Super-resolution multicolor fluorescence microscopy enabled by an apochromatic super-oscillatory lens with extended depth-of-focus
Nature Communications (2023)
-
Structural and functional imaging of brains
Science China Chemistry (2023)
-
Imaging whole-brain activity to understand behaviour
Nature Reviews Physics (2022)
-
Practical considerations for quantitative light sheet fluorescence microscopy
Nature Methods (2022)
-
Multicolor strategies for investigating clonal expansion and tissue plasticity
Cellular and Molecular Life Sciences (2022)