Research Highlights

A rapid method for isolating mitochondria with exquisitely high purity helps researchers profile metabolic dynamics within this organelle.

In the mouse brain, researchers use sequencing to map long-range neuronal projections...a lot of projections.

Sequential rounds of immunoprecipitation and barcoding reveal the genome-wide occurrence of paired histone marks.

A combination of quantitative mass spectrometry, subcellular fractionation and stringent statistical analyses allows the description of protein translocation events at the proteome scale.

A simple tweak on the zygote's mitotic spindle generated hybrid Caenorhabditis elegans with certain tissues (such as those of the nervous system) consisting of entirely maternal genomes and others (such as the germline) consisting of entirely paternal genomes.

Point spread function engineering streamlines multicolor super-resolution imaging and single-particle tracking.

A combination of restricting channel-rhodopsin to the soma and restricting the excitation volume with temporal focusing enables the mapping of neural circuits with high precision.

The Global Natural Products Social Molecular Networking knowledgebase facilitates community sharing and curation of mass spectrometry data from natural products.

Scientists propose a more human-centric benchmark for assessing naive and primed pluripotency in human embryonic stem cells.

By capturing and barcoding RNA in its native tissue location, researchers can visualize and quantify gene expression in situ.

SRMAtlas contains mass spectrometry assays allowing targeted analysis of nearly 100% of human proteins.

By analogy to protein and DNA similarity searches, NBLAST provides a fast and efficient way of finding morphological similarities between neurons.

Single-cell 5hmC sequencing uncovers cell-to-cell differences in the two DNA strands of a given chromosome.

Two new platforms allow single neurons to be imaged throughout the mouse brain.

A proof-of-concept platform demonstrates the feasibility of nanopore-based sequencing of polypeptide chains.

Researchers plumb the depths of the cell with volumetric 4Pi nanoscopy.

Fragmenting transcripts after 4-thiouridine incorporation allows the quantification of even short-lived noncoding RNA.

A fluorescent NAD⁺ biosensor targeted to specific cellular compartments detects local fluctuation in NAD⁺ concentration.

A luciferin analog enables highly sensitive bioluminescent imaging from deep within biological tissue samples.

Nanobodies that are stable only in the presence of their antigen can be harnessed to manipulate or detect antigen-expressing cells.