Research Highlights

Studies of tumor immunology will gain from coculture of tumor organoids and peripheral blood cells.

By coupling multiplex iterative indirect immunofluorescence imaging with computer vision methods, researchers can detect at least 40 different proteins with subcellular resolution.

By incorporating an image-classification task into an online video game, the Human Protein Atlas project unlocks the gaming community as a citizen science force.

A high-throughput platform allows biophysical measurements to probe RNA tertiary folding.

The combination of single-particle electron microscopy and mass spectrometry shows potential for surveys of both the structure and the identity of protein complexes in the cell.

FRET improves the photostability of fluorophores for longer single-molecule tracking.

A genetically encoded sensor based on G-protein-coupled receptors can detect the neurotransmitter acetylcholine in vitro and in vivo.

Independent efforts shine light on the 3D genome structure by looking at multiple contacts along an allele or equalizing the distance between restriction sites for higher-resolution Hi-C maps.

The genetically encoded sensor dLight1 reports dopamine release in culture, slices and behaving mice.

Reversible primer termination, enabled by polymerase–nucleotide conjugates, provides an enzymatic method for the de novo synthesis of oligonucleotides.

Self-assembled nanoparticles give insight into the regulation of macromolecular crowding.

The STARmap method advances RNA sequencing in intact tissues.

Cas9 induces larger-than-anticipated mutations in mouse and human cells. In the latter, efficient editing depends on inhibition of the DNA-damage-repair protein p53.

Two new methods provide high-resolution profiles of splicing events in yeast.

Aberration correction adds depth to light-sheet microscopy.

3C-based methods and polymer modeling indentify the impact of structural variants.

Interferometric scattering microscopy enables quantitative mass imaging and biophysical characterization of single biomolecules in solution.

A combination of behavioral analysis and neural activity recording provides a glimpse into how the brain orchestrates complex behaviors.

Inside a mouse brain, human cerebral organoids can show their potential.

Biobeam is a software package that simulates optical effects during imaging within scattering tissue, which could help improve image acquisition and extraction.