Research Highlights

Searching mass spectra against publicly available tandem MS databases just got easier.

Aerolysin nanopores offer a way to identify natural amino acids and their chemical modifications.

A 3D culture system recapitulates human development prior to gastrulation and enables transcriptomic studies.

Direct library preparation (DLP) for amplification-free single-cell whole-genome sequencing has been upgraded to a high-throughput, scalable platform for identification of clonal populations and their genomic features, integrating them with single-cell morphology.

A method using CRISPR–Cas technology, nanopore sequencing and bioinformatics enables quantification of short tandem repeats with high accuracy.

A protein co-regulation database facilitates proteome-wide functional annotations.

An optical gating strategy allows day-long light-sheet imaging of the developing heart in zebrafish embryos.

Combining a voltage sensor with random-access microscopy allows imaging of neuronal activity in behaving mice.

Stereochemically defined chemical probes aid discovery of ligandable proteins.

Researchers show that mouse stem and adult cells can develop into blastocyst-like structures.

Higher-order processes can be harnessed for super-resolution CARS vibrational microscopy.

Mice implanted with human lung tissue model pathogen infection and immune responses.

An in silico approach helps researchers identify biosynthetic gene clusters coding for bioactive small molecules from metagenomic data of the human microbiome.

Researchers develop a genome-informed approach to isolate and grow targeted cells into pure cultures from microbial communities.

The hybrid voltage-indicator Voltron combines the voltage sensitivity of microbial rhodopsins with the brightness and photostability of chemical dyes.

In silico modeling and mapping of cross-species protein–protein interactions.

Independent studies introduced two distinct tools, both called ‘Pepper’, for live imaging of RNA.

Two teams develop methods for genealogy inference using large genomic datasets.

Chemomagnetics enable remote and non-invasive modulation of neural activity in behaving mice.

The CRISPR–Cas system has become a powerful tool in genome editing and now expands its footprint into materials science.