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A rescanning microscopy approach enables two-photon image-scanning microscopy that doubles resolution relative to that of conventional two-photon microscopy at high frame rates and with high sensitivity for improved super-resolution imaging of living specimens.
A reverse two-hybrid approach combined with massively parallel programmed mutagenesis and sequencing allows profiling protein interaction determinants at amino-acid resolution, as demonstrated by identifying interaction-disrupting mutations in a cilia protein complex associated with Bardet–Biedl syndrome.
Combining each sgRNA with a unique molecular identifier in a genome-wide screen increases sensitivity and robustness in both positive and negative selection.
Enrichment of biotinylated peptides using an anti-biotin antibody results in substantially improved biotinylation site identifications by mass spectrometry compared to traditional streptavidin-based biotinylated protein enrichment.
Cryo-EM-based structure determination of macromolecular complexes at near-atomic resolution is possible using a mid-range 200-keV transmission electron microscope instrument.
The Critical Assessment of Metagenome Interpretation (CAMI) community initiative presents results from its first challenge, a rigorous benchmarking of software for metagenome assembly, binning and taxonomic profiling.
NeuBtracker is an open-source platform that enables simultaneous imaging of behavior and neural activity in freely behaving zebrafish larvae. Its performance is demonstrated during several spontaneous or stimulus-induced behaviors.