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  • This Month |

    A platform for exploring GPCR secrets, powered by community, music and sports.

    • Vivien Marx

  • Article |

    NanoLuc substrates with improved solubility and bioavailability, hydrofurimazine and fluorofurimazine, strongly enhance bioluminescence signals in vivo and enable bright dual-color bioluminescent imaging with AkaLuc and AkaLumine.

    • Yichi Su
    • , Joel R. Walker
    • , Yunhee Park
    • , Thomas P. Smith
    • , Lan Xiang Liu
    • , Mary P. Hall
    • , Louai Labanieh
    • , Robin Hurst
    • , David C. Wang
    • , Lance P. Encell
    • , Namdoo Kim
    • , Feijie Zhang
    • , Mark A. Kay
    • , Kerriann M. Casey
    • , Robbie G. Majzner
    • , Jennifer R. Cochran
    • , Crystal L. Mackall
    • , Thomas A. Kirkland
    •  & Michael Z. Lin

  • Resource |

    GPCRmd is a community-driven online platform to visualize, analyze and share G-protein-coupled receptor (GPCR) molecular dynamics data. It currently contains simulation data representing 100% of GPCR classes, 71% of receptor subtypes and 80% of GPCR families.

    • Ismael Rodríguez-Espigares
    • , Mariona Torrens-Fontanals
    • , Johanna K. S. Tiemann
    • , David Aranda-García
    • , Juan Manuel Ramírez-Anguita
    • , Tomasz Maciej Stepniewski
    • , Nathalie Worp
    • , Alejandro Varela-Rial
    • , Adrián Morales-Pastor
    • , Brian Medel-Lacruz
    • , Gáspár Pándy-Szekeres
    • , Eduardo Mayol
    • , Toni Giorgino
    • , Jens Carlsson
    • , Xavier Deupi
    • , Slawomir Filipek
    • , Marta Filizola
    • , José Carlos Gómez-Tamayo
    • , Angel Gonzalez
    • , Hugo Gutiérrez-de-Terán
    • , Mireia Jiménez-Rosés
    • , Willem Jespers
    • , Jon Kapla
    • , George Khelashvili
    • , Peter Kolb
    • , Dorota Latek
    • , Maria Marti-Solano
    • , Pierre Matricon
    • , Minos-Timotheos Matsoukas
    • , Przemyslaw Miszta
    • , Mireia Olivella
    • , Laura Perez-Benito
    • , Davide Provasi
    • , Santiago Ríos
    • , Iván R. Torrecillas
    • , Jessica Sallander
    • , Agnieszka Sztyler
    • , Silvana Vasile
    • , Harel Weinstein
    • , Ulrich Zachariae
    • , Peter W. Hildebrand
    • , Gianni De Fabritiis
    • , Ferran Sanz
    • , David E. Gloriam
    • , Arnau Cordomi
    • , Ramon Guixà-González
    •  & Jana Selent

  • Article |

    ZipSeq uses patterned illumination and photocaged oligonucleotides to serially print barcodes onto live, intact tissues for spatially mapping single cell transcriptomes.

    • Kenneth H. Hu
    • , John P. Eichorst
    • , Chris S. McGinnis
    • , David M. Patterson
    • , Eric D. Chow
    • , Kelly Kersten
    • , Stephen C. Jameson
    • , Zev J. Gartner
    • , Arjun A. Rao
    •  & Matthew F. Krummel

  • Review Article |

    A review on recent method development for discovering T cell antigens.

    • Alok V. Joglekar
    •  & Guideng Li

  • Research Highlight |

    Proteins that control the optical properties of cephalopod skin cells can modulate the transparency of mammalian cells.

    • Rita Strack

  • Research Highlight |

    Sequencing gradually digested chromatin along the nuclear radius enables the mapping of radial organization of chromatin in human cells.

    • Lei Tang

  • Research Highlight |

    In situ labeling with azido sugars enables in vivo immunomodulation of dendritic cells.

    • Madhura Mukhopadhyay

  • News & Views |

    Producing reliable atomic- or close-to-atomic-resolution structures of RNA-only molecules has been a formidable task. Ribosolve can solve sub-nanometer-resolution cryo-EM structures of unbound RNA molecules with unprecedented accuracy and speed.

    • Jane S. Richardson

  • Editorial |

    Systemic racism has held back under-represented minorities, especially Black people, in scientific research. We’re committed in our roles as journal editors to help correct this inequality.

  • Article |

    The Ribosolve pipeline combines single-particle cryo-EM, M2-seq biochemical analysis and Rosetta auto-DRRAFTER modeling to guide three-dimensional RNA structure determination.

    • Kalli Kappel
    • , Kaiming Zhang
    • , Zhaoming Su
    • , Andrew M. Watkins
    • , Wipapat Kladwang
    • , Shanshan Li
    • , Grigore Pintilie
    • , Ved V. Topkar
    • , Ramya Rangan
    • , Ivan N. Zheludev
    • , Joseph D. Yesselman
    • , Wah Chiu
    •  & Rhiju Das

  • Brief Communication |

    Hundred-fold-faster DNA-PAINT imaging is enabled by the introduction of concatenated, periodic DNA sequence motifs in the docking strand. Six orthogonal sequences are described for speed-optimized and highly multiplexed cellular imaging.

    • Sebastian Strauss
    •  & Ralf Jungmann

  • Article |

    Small vibrational tags (azide, 13C-edited carbonyl and deuterium-labeled probes) were introduced as metabolic probes for mid-infrared imaging. The tags allow unprecedented in situ visualization of metabolism in cells and animals with high information throughput.

    • Lixue Shi
    • , Xinwen Liu
    • , Lingyan Shi
    • , H. Ted Stinson
    • , Jeremy Rowlette
    • , Lisa J. Kahl
    • , Christopher R. Evans
    • , Chaogu Zheng
    • , Lars E. P. Dietrich
    •  & Wei Min

  • Article |

    PULSE is an optogenetic tool that consists of two modules with different wavelength sensitivities. Their interplay enables optogenetic access to gene expression in plants independently of ambient light.

    • Rocio Ochoa-Fernandez
    • , Nikolaj B. Abel
    • , Franz-Georg Wieland
    • , Jenia Schlegel
    • , Leonie-Alexa Koch
    • , J. Benjamin Miller
    • , Raphael Engesser
    • , Giovanni Giuriani
    • , Simon M. Brandl
    • , Jens Timmer
    • , Wilfried Weber
    • , Thomas Ott
    • , Rüdiger Simon
    •  & Matias D. Zurbriggen

  • Brief Communication |

    CARPID uses CRISPR technology to navigate biotin ligase to specific lncRNAs, which allows proximal labeling and thus the querying of RNA–protein interactions in living cells.

    • Wenkai Yi
    • , Jingyu Li
    • , Xiaoxuan Zhu
    • , Xi Wang
    • , Ligang Fan
    • , Wenju Sun
    • , Linbu Liao
    • , Jilin Zhang
    • , Xiaoyu Li
    • , Jing Ye
    • , Fulin Chen
    • , Jussi Taipale
    • , Kui Ming Chan
    • , Liang Zhang
    •  & Jian Yan

  • Article |

    Multifocal flat illumination for field-independent imaging (mfFIFI) enables patterned illumination over an extended field of view. Integration with instant structured illumination microscope allowed for high-speed, multicolor, volumetric super-resolution imaging over 100 × 100 µm2.

    • Dora Mahecic
    • , Davide Gambarotto
    • , Kyle M. Douglass
    • , Denis Fortun
    • , Niccoló Banterle
    • , Khalid A. Ibrahim
    • , Maeva Le Guennec
    • , Pierre Gönczy
    • , Virginie Hamel
    • , Paul Guichard
    •  & Suliana Manley

  • This Month |

    A yellow fluorescent sensor to study the brain, and the joy and pain of climbing.

    • Vivien Marx

  • Brief Communication |

    Split-FISH leverages a split-probe design to achieve enhanced specificity in multiplexed fluorescence in situ hybridization. Split-FISH reduces background and false positives, enabling transcriptome profiling in uncleared tissues.

    • Jolene Jie Lin Goh
    • , Nigel Chou
    • , Wan Yi Seow
    • , Norbert Ha
    • , Chung Pui Paul Cheng
    • , Yun-Ching Chang
    • , Ziqing Winston Zhao
    •  & Kok Hao Chen

  • Article |

    DeepSTORM3D uses deep learning for accurate localization of point emitters in densely labeled samples in three dimensions for volumetric localization microscopy with high temporal resolution, as well as for optimal point-spread function design.

    • Elias Nehme
    • , Daniel Freedman
    • , Racheli Gordon
    • , Boris Ferdman
    • , Lucien E. Weiss
    • , Onit Alalouf
    • , Tal Naor
    • , Reut Orange
    • , Tomer Michaeli
    •  & Yoav Shechtman

  • Perspective |

    This Perspective highlights recently developed computational models for studying chromosome organization, with a focus on how mechanistic modeling helps biologists to interpret the biological function behind the genome structures.

    • Chris A. Brackey
    • , Davide Marenduzzo
    •  & Nick Gilbert

  • Article |

    Guide RNA selection plays an essential role in CRISPR screens, the Vienna Bioactivity CRISPR (VBC) scoring system provides an improved selection for sgRNAs that generate loss-of-function alleles.

    • Georg Michlits
    • , Julian Jude
    • , Matthias Hinterndorfer
    • , Melanie de Almeida
    • , Gintautas Vainorius
    • , Maria Hubmann
    • , Tobias Neumann
    • , Alexander Schleiffer
    • , Thomas Rainer Burkard
    • , Michaela Fellner
    • , Max Gijsbertsen
    • , Anna Traunbauer
    • , Johannes Zuber
    •  & Ulrich Elling

  • Research Highlight |

    Embryonic development is typically hidden from view, but a window preparation technique now sheds light on this phase in the life of a mouse.

    • Nina Vogt

  • Research Highlight |

    De novo–designed protein logic gates allow post-translational regulation of protein function.

    • Arunima Singh

  • Research Highlight |

    Improvements to the Genome Taxonomy Database provide a complete bacterial and archaeal taxonomy.

    • Lin Tang